Cryopreservation of In Vitro. Grown Apical Shoot Tips of Strawberry by Vitrification.

Niino, Toshiki; Tanaka, Daisuke; Ichikawa, S.; Takano, Junichi; Ivette, Seguel; Shirata, Kazuto; Uemura, Matsuo

doi:10.5511/plantbiotechnology.20.75

Cited by 22 publications

(18 citation statements)

References 7 publications

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“…As such, there is no need of cold acclimation in carnation. Secondly, preculture on MS medium with high sucrose concentrations and osmoprotection was effective for the induction of osmotolerance towards PVS2 (Niino et al 2003;Niino et al 2007). In potato cryopreservation by encapsulation vitrification, the LS solution (a mixture of 2 M glycerol plus 0.6 M sucrose) was effective in increasing osmotolerance towards PVS2 (Hirai and Sakai 1999).…”

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Cryopreservation of in vitro-grown shoot tips of carnation (Dianthus caryophyllus L.) by vitrification method using aluminium cryo-plates

Sekizawa

Yamamoto

Rafique

et al. 2011

Plant Biotechnology

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Cryopreservation using an aluminum cryo-plate was successfully applied to in vitro-grown carnation (Dianthus caryophyllus L.) shoot tips. The shoot tips (1-1.5 mmϫ1 mm) were dissected from the shoot and precultured at 25°C for 2 days on MS medium containing 0.3 M sucrose. The precultured shoot tips were placed on the aluminum cryo-plate containing ten wells embedded with alginate gel. Osmoprotection was performed by immersing the cryo-plates in loading solution (2 M glycerol and 1.4 M sucrose) for 90 min at 25°C. Then, dehydration was performed by immersing the cryoplates in PVS2 for 25 min at 25°C. After storage in liquid nitrogen, shoot tips attached to cryoplate, were directly immersed into 2 ml 1 M sucrose solution for regeneration. Using this procedure, the average regrowth level of vitrified shoot tips of 4 carnation cultivars reached 95%. This new method has many advantages and will facilitate the cryostorage of reference cultivars of carnation. (LS) and PVS2 without floating and/or clinging of shoot tips and also with no or low level of injury to those shoot tips. In this paper, this V-Cryo-plate procedure was applied for the cryopreservation of carnation.The carnation plants used in this study were obtained from the Nishinihon Station, NCSS, sub-bank of NIAS Genebank Project, in Japan in April 2010. Four cultivars of carnation, 'Melissa', 'Grana', 'Terra Cotta' and 'Yell', were tested. Apical shoots, excised from in vivo explants, were surface-sterilized successively with 70% ethanol for 1 min, and 0.5% sodium hypochlorite for 10 min, and then rinsed twice with sterilized distilled water. After surface sterilization, shoot tips with basal plates (1 mm longϫ1 mm wide) were excised from the shoots and cultured on 70 ml of 0.4% (w/v) gellan gum-solidified MS medium (Murashige, Skoog 1962) in a culture flask (80 mmϫ100 mm) containing 1 mg l Ϫ1 thidiazuron, 1 mg l Ϫ1 naphthalene acetic acid and 3% (w/v) sucrose (Jose et al. 2010). After 50 days of culture, shoots were transferred onto gellan gum-solidified MS medium devoid of plant growth regulators. Then stock plants were subcultured every two months on MS medium. Cultures were incubated at 25°C with a 16 h photoperiod under white fluorescent light (52 mmol m Ϫ2 s Ϫ1) in the culture flask (standard condition). For cryopreservation of in vitro shoot tips of carnation, the V-Cryo-plate procedure was applied. The size of the aluminum cryoplate used was 7 mmϫ37 mmϫ0.5 mm with ten wells (diameter 1.5 mm, depth 0.75 mm) ( Figure 1B). These plates fit in 2 ml cryotube. The different steps of the VCryo-plate procedure are as follows; 1) Cut shoots (5 mm) with a lateral bud and plate on solidified MS medium and culture for 2 weeks at 25°C in standard conditions ( Figure 1A). Then, dissect shoot tips with basal plate (1-1.5 mm longϫ1 mm wide) from the shoots and preculture for 2 days at 25°C on the MS medium with 0.3 M sucrose. 2) Place an aluminum cryo-plate in Petri-dish and pour 2.0-2.5 ml 2% (w/v) Na-alginate solution with 0.4 M sucrose in calcium-free M...

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confidence: 99%

Cryopreservation of in vitro-grown shoot tips of carnation (Dianthus caryophyllus L.) by vitrification method using aluminium cryo-plates

Sekizawa

Yamamoto

Rafique

et al. 2011

Plant Biotechnology

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“…And some plant species are moved gradually from low to high concentration of sucrose medium Niino & Sakai, 1992a,b;Suzuki et al, 1994;Niino et al, 1997). In addition, there are also cases in which glycerol (Matsumoto et al, 1998;Niino et al, 2003), DMSO (Fukai, 1990), or abscisic acid (ABA; Kendal et al, 1993;Tsukazaki et al,2000) is mixed with a sucrose culture medium, and culture medium containing sorbitol without sucrose are used (Yamada et al, 1991;Maruyama et al, 2000). In many cases, room temperature is used for treatment (20~25 o C).…”

Section: Treatment Before Cryopreservationmentioning

confidence: 99%

“…Thus, this may threaten the survival of plant specimens after cryopreservation. In that case, regeneration of tissues after preservation reportedly increased when activated charcoal (Bagniol & Engelmann, 1992) and polyvinyl pyrrolidone (Niino et al, 2003), an adsorbent of polyphenol, was mixed with a culture medium.…”

Section: Treatment After Cryopreservationmentioning

confidence: 99%

Cryopreservation of Plant Genetic Resources

Kami¹

2012

Current Frontiers in Cryobiology

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“…These dramatically increase applicability to a wide range of plant germplasm, especially in non-hardy materials and plants. For example, we developed a practical cryopreservation method of in vitro grown strawberry shoot tips by vitrification 5 . Using this protocol, we have already cryopreserved about 60 strawberry accessions.…”

Section: Cryopreservation Of Vegetatively Propagated Cropsmentioning

confidence: 99%

Plant Genetic Resources in Japan: Platforms and Destinations to Conserve and Utilize Plant Genetic Diversity

Okuno

Shirata

Niino

et al. 2005

JARQ

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Since 1985, the Genebank project has been implemented as a national program to collect, characterize, evaluate, rejuvenate, conserve and use plant, microorganism and animal genetic resources for food and agriculture. NIAS has functioned as a center-bank of the project in cooperation with other agricultural public and private sectors. This national network on genetic resources for food and agriculture currently preserves approximately 233,000 accessions of crop germplasm and wild relatives of about 1,450 species involving 45,000 accessions of clonal crops. Among them 205,000 accessions are conserved as base collection, while 129,000 accessions, as active collection, are distributed for research purposes under the regulation of MTA between NIAS Genebank and recipients worldwide. We have undertaken international collaboration focusing on exploration for collecting genetic resources under the regulation of MOU or MOA with collaborating parties and on in situ conservation and on farm management of plant genetic resources in several Asian countries. Research highlights on conservation and utilization of plant genetic resources will be discussed in this report.

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Cryopreservation of In Vitro. Grown Apical Shoot Tips of Strawberry by Vitrification.

Cited by 22 publications

References 7 publications

Cryopreservation of in vitro-grown shoot tips of carnation (Dianthus caryophyllus L.) by vitrification method using aluminium cryo-plates

Cryopreservation of in vitro-grown shoot tips of carnation (Dianthus caryophyllus L.) by vitrification method using aluminium cryo-plates

Cryopreservation of Plant Genetic Resources

Plant Genetic Resources in Japan: Platforms and Destinations to Conserve and Utilize Plant Genetic Diversity

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