Cryopreservation of invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure

Takagi, H.; Thịnh, Nguyễn Tiến; Islam, O. M.; Senboku, Toshihiro; Sakai, Akira

doi:10.1007/bf01275498

Cited by 69 publications

(8 citation statements)

References 9 publications

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“…e average regeneration of vitrified meristems aer storage in LN was 63.7%, ranging from 57.5% to 82.5%, which was significantly higher than that of the encapsulation-dehydration method (22.5%) and DMSO droplet method (28.8%). By optimizing the vitrification of shoot tips procedures, the percentage of regeneration of smaller meristems (1 mm in length) was significantly higher (63.7%) than that of meristems 3 mm in length (30.4%), which was consistent with the reports of Escobar et al (2000) for cassava and Takagi et al (1997) for taro, and was contrary to the study of Schäfer-Menuhr et al (1996) for potato tips cryopreserved using the droplet method, where the larger meristems had a higher regeneration rate. Kryszczuk (2005) also demonstrated that the incubation time in a cryoprotectant (PVS2) influenced the regeneration efficiency and was dependent on the size of the explants.…”

Section: Solanum Tuberosum Lsupporting

confidence: 90%

Cryopreservation of Plant Tissues in Poland: Research Contributions, Current Status, and Applications

et al. 2022

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Cryopreservation of vegetatively propagated plant material is an increasingly widely used method for the efficient and safe storage of germplasm resources around the world. In Poland, there are currently four cryobanks in use for long-term plant protection programs. However, plant tissues propagated in vitro constitute only a small portion of the accessions stored in them. To date, cryogenic storage techniques have been developed and adopted in this country for ornamental plants (roses, chrysanthemums, and geophytes), crop species (potato and garlic), forest tree species (the genera Quercus and Fraxinus ), and some ferns. Polish researchers have used suspension cultures of Gentiana spp. and shoot tips of Lamprocapnos spectabilis to improve cryopreservation knowledge. A better understanding of the benefits of cryopreservation and its widespread implementation in plant biodiversity conservation programs is required. The objective of this review is to provide a concise synthesis of the scientific contributions, current status, and applications of cryogenic techniques for the conservation of in vitro culture-derived plant tissues in Poland. First, the results contributing to research that has been achieved using cell suspensions and advances related to the use of nanoparticles and plant extracts to improve cryopreservation efficiency are discussed. Then, the applications and advances in cryopreservation of ornamental plants (roses, radiomutants, plant chimeras, Lamprocapnos spp., and geophytes), crop species (potato and garlic), forest trees, and ferns are summarized.

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Section: Solanum Tuberosum Lsupporting

confidence: 90%

Cryopreservation of Plant Tissues in Poland: Research Contributions, Current Status, and Applications

et al. 2022

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“…Many cryopreserved medicinal plants have optimal regrowth when exposed for 20 min to PVS2 [14, 25]. However, 10–25 min exposure duration to PVS2 at 25°C was shown to be optimal for several herbaceous plant species [24, 26]. It is clear from these studies that the acquisition of tolerance to PVS2 is necessary for survival after cryopreservation and also that the exposure duration may be genotype dependent.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Shoot Tips of Elite Cultivars of <b><i>Cannabis sativa</i></b> L. by Droplet Vitrification

Uchendu

Lata

Chandra

et al. 2019

Med Cannabis Cannabinoids

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Cannabis sativa L. (marijuana or hemp) is recognized worldwide for its psychoactive properties as well as for fiber production. This study focused on the evaluation of 3 droplet vitrification protocols for long-term conservation of shoot tips in liquid nitrogen (LN). Shoot tips (∼0.5 mm) were excised from 3- to 4-week-old in vitro-grown shoots of 3 cultivars (MX, VI-20, and B-5: high tetrahydrocannabinol [THC], high cannabidiol [CBD], and intermediate THC∼CBD, respectively) and pretreated on 5% dimethyl sulfoxide agar plates for 48 h. The shoot tips were then vitrified in LN using 3 separate cryoprotectant (plant vitrification solutions [PVS] #2, #3, and #4) droplets on an aluminum cryoplate. There was no significant difference between the regrowth of cryopreserved shoot tips exposed to PVS2 for 15 and 20 min, but regrowth of all 3 cultivars significantly declined after 20 min of exposure. Exposure duration of 15 min was adapted for subsequent experiments. Regrowth of cryopreserved MX was significantly higher with PVS2 (63%) than with PVS3 and PVS4 (≤5%). Regrowth of cryopreserved VI-20 was highest with PVS2 (57%) and significantly higher than with PVS3 and PVS4 (≤25%). The regrowth of cryopreserved shoot tips of B-5 was significantly different between all 3 protocols with PVS2 > PVS4 > PVS3. Both PVS2 and PVS4 produced regrowth above 55%, while regrowth with PVS3 was significantly lower (31%). These results indicate that 15–20 min of exposure to PVS2 are most suitable for cryopreservation of these varieties. This is the first report on protocol development for the cryopreservation of organized tissues of C. sativa L. for germplasm conservation.

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“…While the Vi method was shown to achieve satisfactory shoot tip regrowth in yam [74,117,126] and taro [127], a further improved shoot tip regrowth was achieved using other vitrification solution-based methods [108,124,128]. Studies making use of Vi protocol for potato cryopreservation were compared with other Vi-based methods in Figure 1.…”

Section: Dehydration Cryo-plate Methods (D Cryo-plate)mentioning

confidence: 99%

Overcoming Challenges for Shoot Tip Cryopreservation of Root and Tuber Crops

Zhang

Wang

et al. 2023

Agronomy

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Root and tuber crops (RTCs) are the second-most important carbohydrate commodity after cereals. Many species of the RTCs are vegetatively propagated, making their shoot tips the preferred material to be conserved for future uses. Shoot tip cryopreservation provides an important tool to support the long-term conservation of plant genetic resources. Over the past four decades, significant efforts have been undertaken to move shoot tip cryopreservation of RTCs from research projects to full-scale implementation in cryobanks. This comprehensive review focuses on the history of cryopreservation protocols developed in RTCs. The encapsulation and vitrification solution-based cryopreservation techniques followed by ultra-rapid freezing and thawing have been highly successful. Additionally, different strategies for improving the cryotolerance of shoot tips have been introduced to further increase post-cryopreservation recovery. Finally, the research conducted to explain the mechanism underlying cryoprotection and differential cryotolerance including the use of histological studies are highlighted.

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Cryopreservation of invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure

Cited by 69 publications

References 9 publications

Cryopreservation of Plant Tissues in Poland: Research Contributions, Current Status, and Applications

Cryopreservation of Plant Tissues in Poland: Research Contributions, Current Status, and Applications

Cryopreservation of Shoot Tips of Elite Cultivars of <b><i>Cannabis sativa</i></b> L. by Droplet Vitrification

Overcoming Challenges for Shoot Tip Cryopreservation of Root and Tuber Crops

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