Cryopreservation of Italian cultivars of hazelnut

Sgueglia, A.; Gentile, A.; Frattarelli, A.; Germanà, María Antonietta; Caboni, E.

doi:10.17660/actahortic.2021.1307.25

Cited by 7 publications

(3 citation statements)

References 12 publications

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“…An auxin-type compound, 2-naphthoxyacetic acid, was successfully used at a concentration of 1 mg L −1 to recover embryogenic cell suspensions of several Vitis cultivars, leading to survival ranging from 42 to 82% and subsequent plant regeneration [ 230 ]. Meta-Topolin [N6-(3-hydroxybenzyl)adenine], an aromatic cytokinin, was found to be effective during the recovery of Corylus avellana buds and resulted in the same or significantly better regrowth than BA, depending on cultivar [ 231 ]. Meta-Topolin is reported to increase in vitro shoot production, reduce abnormalities during development, and alleviate shoot tip necrosis in delayed senescence in different plant species [ 232 ].…”

Section: Strategy 4 Plant Growth Regulators and Their Combinations At...mentioning

confidence: 99%

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Попова

Куличенко

Kim

2023

Biology

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Cryopreservation is an effective option for the long-term conservation of plant genetic resources, including vegetatively propagated crops and ornamental plants, elite tree genotypes, threatened plant species with non-orthodox seeds or limited seed availability, as well as cell and root cultures useful for biotechnology. With increasing success, an arsenal of cryopreservation methods has been developed and applied to many species and material types. However, severe damage to plant material accumulating during the multi-step cryopreservation procedure often causes reduced survival and low regrowth, even when the optimized protocol is applied. The conditions at the recovery stage play a vital role in supporting material regrowth after cryopreservation and, when optimized, may shift the life-and-death balance toward a positive outcome. In this contribution, we provide an overview of the five main strategies available at the recovery stage to improve post-cryopreservation survival of in vitro plant materials and their further proliferation and development. In particular, we discuss the modification of the recovery medium composition (iron- and ammonium-free), exogenous additives to cope with oxidative stress and absorb toxic chemicals, and the modulation of medium osmotic potential. Special attention is paid to plant growth regulators used at various steps of the recovery process to induce the desired morphological response in cryopreserved tissues. Given studies on electron transport and energy provision in rewarmed materials, we discuss the effects of light-and-dark conditions and light quality. We hope that this summary provides a helpful guideline and a set of references for choosing the recovery conditions for plant species that have not been cryopreserved. We also propose that step-wise recovery may be most effective for materials sensitive to cryopreservation-induced osmotic and chemical stresses.

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Section: Strategy 4 Plant Growth Regulators and Their Combinations At...mentioning

confidence: 99%

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Попова

Куличенко

Kim

2023

Biology

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“…The traditional nursery propagation method of hazelnuts is based on the use of suckers from mound layering, a time-consuming technique with low efficiency at the commercial level ( Mardani et al, 2020 ). Therefore, the micropropagation method is not only considered as an appropriate alternative for producing high quality, disease-free, and true-to-type hazelnut plants, independent from the season and environmental conditions ( Debergh et al, 1991 ), but also it represents a valuable tool for plant biodiversity conservation ( Sgueglia et al, 2018 , 2019 ). However, hazelnut genotypes commonly show a difficult in vitro establishment due to surface sterilization failure, strictly related to the very common problem of endogenous contamination, showing even after weeks or months after culture initiation, besides to the lack of young plant material ( Hand et al, 2016 ; Javed et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Is It Possible to Produce Certified Hazelnut Plant Material in Sicily? Identification and Recovery of Nebrodi Genetic Resources, in vitro Establishment, and Innovative Sanitation Technique From Apple Mosaic Virus

et al. 2021

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Eight Sicilian cultivars of hazelnut (Corylus avellana L.), namely-Curcia, Nociara Collica, Panottara Collica, Panottara Galati Grande, Parrinara, Panottara Baratta Piccola, Enzo, and Rossa Galvagno, registered into the Italian Cultivar Register of fruit tree species in 2017 were selected from Nebrodi area and established in vitro. The aim of the work was to carry out the sanitation of the cultivars and get virus-free plants from the most important viral pathogen threat, the apple mosaic virus. Virus-free plant material is essential for the production of certified plants from Sicilian hazelnut cultivars, complying the CE (cat. CAC) quality and the technical standards established in 2017 for voluntary certification by the Italian Ministry of Agricultural, Food and Forestry Policies (MIPAAF). In this study, we investigated the possibility of establishing in vitro true-to-type and virus-free hazelnut plantlets via the encapsulation technology of apexes. The in vitro shoot proliferation rates were assessed for the different cultivars, sampling periods, temperature treatments, and type of explant used for culture initiation. Viability, regrowth, and conversion rates of both conventional meristem tip culture (MTC) and not conventional (MTC combined with the encapsulation technology) sanitation techniques were evaluated.

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“…However, since heterozygosity is high in hazelnut cultivars, clonal preservation, based on cryopreservation of shoot tips or axillary buds, is much more desirable than seeds or pollen preservation [45][46][47][48][49][50]. Recently, a study performed on cryopreservation of two Italian hazelnut cultivars showed the possibility of the application of an encapsulationdehydration method on axillary buds excised from in vitro growing shoots [51].…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Hazelnut (Corylus avellana L.) Axillary Buds from In Vitro Shoots Using the Droplet Vitrification Method

Sgueglia

Frattarelli²,

Gentile³

et al. 2021

Horticulturae

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Cryopreservation by droplet vitrification was applied to hazelnut (Corylus avellana L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from in vitro growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced regrowth to 41.5%. Increasing the exposure to vitrification solutions to 90 min reduced regrowth to 43.3% when PVS3 was applied, and 35.6% if PVS2 was used. The cold pre-treatment on the mother plant did not significantly improve overall regrowth. The cryopreservation process did not decline the rooting ability of the recovered shoots.

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Cryopreservation of Italian cultivars of hazelnut

Cited by 7 publications

References 12 publications

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Is It Possible to Produce Certified Hazelnut Plant Material in Sicily? Identification and Recovery of Nebrodi Genetic Resources, in vitro Establishment, and Innovative Sanitation Technique From Apple Mosaic Virus

Cryopreservation of Hazelnut (Corylus avellana L.) Axillary Buds from In Vitro Shoots Using the Droplet Vitrification Method

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