Cryopreservation of lar gibbon semen collected by manual stimulation

Takasu, Masaki; Morita, Natsumi; Tajima, Shunichiro; Almunia, Julio; Maeda, Masami; Kamiguchi, Takashi

doi:10.1007/s10329-016-0543-1

Cited by 1 publication

(1 citation statement)

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“…This has been observed in several species including domestic stallions (Equus caballus) (Cary et al, 2004) and grey wolf (Christensen et al, 2011). MS and AV in wild species require intensive animal training and conditioning, which has been successfully performed on captive whales (Robeck et al, 2010;Montano et al, 2012), monkeys (Takasu et al, 2016), and zebra (Crump and Crump, 1994). However, MS leads to other complications as seen in Asian elephant (Elephas maximus) where the mix of seminal plasma components can vary with each ejaculate and the risk of urine contamination is increased (Imrat et al, 2012).…”

Section: Extraction Methodsmentioning

confidence: 99%

Strong Heterogeneity in Advances in Cryopreservation Techniques in the Mammalian Orders

et al. 2018

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Between 1970 and 2012, vertebrate abundance has declined by 58% with an average annual decline of 2%, calling for serious action to prevent a mass extinction and an irreversible loss of biodiversity. Cryobanks and cryopreservation have the potential to assist and improve ex situ and in situ conservation strategies by storing valuable genetic material. A great deal of studies concerning cryopreservation have been performed within the class Mammalia, although no systematic overview has previously been presented. The objective of this study is therefore to evaluate the status, pattern and future of cryopreservation within Mammalia. A strong disproportional distribution of studies in examined orders is displayed. For the majority of examined orders less than 10% of species has been examined. However, the cryopreservation of germplasm has in several cases been successful and resulted in successful applications of assisted reproductive techniques (ARTs). Various obstacles are associated with the development of cryopreservation protocols, and among them the most prominent is interspecific differences in cryotolerance. Extrapolation of protocols in closely related species is considered the most applicable procedure, and a future supplement to overcome this problem is the examination and comparison of cryobiological traits. Successful protocols have been developed for the vast majority of domesticated mammals, which gives incentive for the further extrapolation of protocols in threatened species.

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Section: Extraction Methodsmentioning

confidence: 99%