Cryopreservation of papaya seeds cv. Sukma, Callina, and Caliso: Effect of loading treatment and immersion time in plant vitrification solution-2

Wardani, Fitri Fatma; Efendi, Darda; Dinarti, Diny; Witono, Joko Ridho

doi:10.13057/nusbiosci/n110112

Cited by 3 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This strategy has been used successfully to preserve seeds of rainforest-origin plants such as Australian Citrus spp. (Rutaceae) [126], Carica papaya cultivars (Caricaceae) [127], and Coffea spp. (Rubiaceae) [128].…”

Section: Discussionmentioning

confidence: 99%

Myrtaceae in Australia: Use of Cryobiotechnologies for the Conservation of a Significant Plant Family under Threat

Hardstaff

Sommerville

Funnekotter

et al. 2022

Plants

View full text Add to dashboard Cite

The Myrtaceae is a very large and diverse family containing a number of economically and ecologically valuable species. In Australia, the family contains approximately 1700 species from 70 genera and is structurally and floristically dominant in many diverse ecosystems. In addition to threats from habitat fragmentation and increasing rates of natural disasters, infection by myrtle rust caused by Austropuccinia psidii is of significant concern to Australian Myrtaceae species. Repeated infections of new growth have caused host death and suppressed host populations by preventing seed set. Although most Myrtaceae species demonstrate orthodox seed storage behavior, exceptional species such as those with desiccation sensitive seed or from myrtle rust-suppressed populations require alternate conservation strategies such as those offered by cryobiotechnology. Targeting seven key Australian genera, we reviewed the available literature for examples of cryobiotechnology utilized for conservation of Myrtaceae. While there were only limited examples of successful cryopreservation for a few genera in this family, successful cryopreservation of both shoot tips and embryonic axes suggest that cryobiotechnology provides a viable alternative for the conservation of exceptional species and a potential safe storage method for the many Myrtaceae species under threat from A. psidii.

show abstract

Section: Discussionmentioning

confidence: 99%

Myrtaceae in Australia: Use of Cryobiotechnologies for the Conservation of a Significant Plant Family under Threat

Hardstaff

Sommerville

Funnekotter

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

Optimising the PVS2 treatment duration for cryopreserving seeds of the endangered rheophytic orchid Epipactis flava Seidenf

Linjikao,

Inthima,

Limmongkon

et al. 2024

The Journal of Horticultural Science and Biotechnology

View full text Add to dashboard Cite

Effect of Cryo-Storage on Storability of Papaya Seeds

Kavitha,

Subhigha,

Manonmani

et al. 2024

THE JAPS

View full text Add to dashboard Cite

A fruit crop of significant economic importance, papayas are often propagated from seeds. Based on their freezing injury even at low moisture content, papaya seeds are classified as "intermediate seeds"; and rapid loss of viability in storage is assumed to be the primary cause of poor germination; thus, the development of appropriate technology is necessary to prolong the storability of papaya seeds. One such effective technology for prolonged seed storability is cryo-storage. The purpose of the current study was to investigate how cryostorage affects papaya seed storability. For this investigation, TNAU Papaya variety CO 8 seeds were utilised. Five distinct treatments were applied to the seeds, which were then kept for approximately six months at liquid nitrogen's vapour phase (-140°C). The biochemical parameters such as protein content, free amino acid, total carbohydrates, free sugars, total lipids, and dehydrogenase activity were measured on a monthly basis along with seed germination and seedling vigour in the cryopreserved papaya seeds. Results indicated that papaya seeds that had been vitrified (seeds with a 10% moisture content that had been soaked in loading solution for 20 minutes, then immersed in Plant Vitrification Solution 2 (PVS2) for another 20 minutes, packed in cryovials and kept in cryo-storage at -140°C) compared to papaya seeds that were packed in cloth bags and stored in ambient conditions had higher seed germination, dry matter production and vigour index at six months after storage. In comparison to fresh seeds, the results also showed that, after six months of cryo-storage, biochemical proximate, protein content, total carbohydrate content, total lipid content, and dehydrogenase activity were maintained in the aforementioned treatment with minimum reduction, while electrical conductivity, free amino acid content, and free sugar content gradually increased with the storage period at minimum rate. In crux, vitrified papaya seeds packaged in cryovials and kept in cryo-storage at -140°C preserved their quality for six months, meeting Indian Minimum Seed Certification Standards. This could be useful for storing papaya seeds for longer periods of time. Keywords: Papaya, vitrification, differential scanning calorimetry, liquid nitrogen, cryo-storage

show abstract

Cryopreservation of papaya seeds cv. Sukma, Callina, and Caliso: Effect of loading treatment and immersion time in plant vitrification solution-2

Cited by 3 publications

References 14 publications

Myrtaceae in Australia: Use of Cryobiotechnologies for the Conservation of a Significant Plant Family under Threat

Myrtaceae in Australia: Use of Cryobiotechnologies for the Conservation of a Significant Plant Family under Threat

Optimising the PVS2 treatment duration for cryopreserving seeds of the endangered rheophytic orchid Epipactis flava Seidenf

Effect of Cryo-Storage on Storability of Papaya Seeds

Contact Info

Product

Resources

About