Cryopreservation of periodontal ligament cells with magnetic field for tooth banking

Kaku, Masato; Kamada, Hiroki; Kawata, Toshitsugu; Koseki, Hiroyuki; Abedini, Sara; Kojima, Sunao; Motokawa, Masaharu; Fujita, Tadashi; Ohtani, Junji; Tsuka, Natsumi; Matsuda, Yayoi; Sunagawa, Hiroko; Hernandes, R.A.M.; Ohwada, N.; Tanne, Kazuo

doi:10.1016/j.cryobiol.2010.05.003

Cited by 84 publications

(70 citation statements)

References 26 publications

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“…Further investigation of freezing optimization may improve the isolation efficacy of MSCs from cryopreserved tissues, such as a novel programmable freezer coupled to a magnetic field. 16 Currently, most freshly donated UCB units are discarded because of their small volumes and/or cell numbers.…”

Section: Discussionmentioning

confidence: 99%

Isolation of mesenchymal stromal/stem cells from cryopreserved umbilical cord blood cells

Fujii

Miura

Iwasa

et al. 2017

J Clin Exp Hematopathol

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JC EH lin xp ematopathol Original Article INTRODUCTIONCell therapy using human mesenchymal stromal/stem cells (MSCs) has been extensively explored for the treatment of a variety of diseases based on their multiple biological and therapeutic effects.1 An off-the-shelf allogeneic bone marrow (BM)-derived MSC product is currently available in the clinic for the treatment of the intractable acute graft-versushost disease after hematopoietic stem cell transplantation (HSCT). 2,3 BM is the most commonly used source of MSCs. 4 However, invasive procedures are required to obtain BM cells from donors. Although other sources of MSCs that can be obtained without invasive treatments have been sought, including expulsed placenta and exfoliated teeth, complex procedures with enzymatic digestion are necessary to prepare cells from these tissues. 5,6 The demand for allogeneic MSCs is expected to increase in the future; therefore, appropriate sources of MSCs that can be alternatives to BM need to be explored for the further development of MSCbased therapy.In a previous study, we demonstrated that MSCs can be isolated from freshly donated umbilical cord blood (UCB) units that do not qualify for the Japanese banking system as a hematopoietic stem cell (HSC) source because of their small volume.7 UCB has advantages over other tissues because it can be obtained from donors without invasive procedures, and a simple adhesion method without complex procedures can be used to isolate MSCs. In addition, infrastructure for processing and cryopreserving UCB cells is established in the banking system for HSCT in Japan. More importantly, about 90% of donated UCB units are discarded because they do not meet the requirements for an HSC source in terms of volume and/or cell number. 8 We have further extended our investigation of the availability of such UCB units as a Umbilical cord blood (UCB) has advantages over other tissues because it can be obtained without an invasive procedure and complex processing. We explored the availability of cryopreserved UCB cells as a source of mesenchymal stromal/stem cells (MSCs). MSCs were successfully isolated from six of 30 UCB units (median volume, 34.0 mL; median nucleated cell number, 4.4×10 8 ) that were processed and cryopreserved using CP-1/human serum albumin. This isolation rate was lower than that (57%) from non-cryopreserved UCB cells. The number of nucleated cells before and after hydroxyethyl starch separation, UCB unit volume, and cell viability after thawing did not significantly differ between UCB units from which MSCs were successfully isolated and those from which they were not. When CryoSure-DEX40 was used as a cryoprotectant, MSCs were isolated from two of ten UCB units. Logistic regression analysis demonstrated that the cryopreservation method was not significantly associated with the success of MSC isolation. The isolated MSCs had a similar morphology and surface marker expression profile as bone marrow-derived MSCs and were able to differentiate into osteogenic, adipogenic, and chondro...

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Section: Discussionmentioning

confidence: 99%

Isolation of mesenchymal stromal/stem cells from cryopreserved umbilical cord blood cells

Fujii

Miura

Iwasa

et al. 2017

J Clin Exp Hematopathol

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“…The CAS freezer was programmed to freeze cells at 20.5uC/min with a 0.1-mT magnetic field, a 15-minutte hold time at 25uC, and a plunging temperature of 230uC according to our previous study. [10][11][12] Then, the teeth were cryopreserved at 2150uC.…”

Section: Treatment Progressmentioning

confidence: 99%

“…A CAS freezer can prevent ice crystal formation, and our previous findings showed that a 0.1-mT magnetic field, a 15-minute hold time, and a plunging temperature of 230uC led to the greatest survival rate for PDL cells. 10 However, autotransplantation of human cryopreserved teeth by a CAS freezer has not yet been performed. This case report describes the treatment of a skeletal Class III malocclusion with a tooth autotransplanted after cryopreservation for 6 years.…”

Section: Introductionmentioning

confidence: 99%

A case of tooth autotransplantation after long-term cryopreservation using a programmed freezer with a magnetic field

Kaku

Shimasue

Ohtani³

et al. 2014

The Angle Orthodontist

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This case report describes the treatment of a skeletal Class III malocclusion with autotransplantation of a cryopreserved tooth. To gain an esthetic facial profile and good occlusion, extraction of bimaxillary premolars and surgical therapy were chosen. The patient had chronic apical periodontitis on the lower left first molar. Although she did not feel any pain in that region, the tooth was considered to have a poor prognosis. Therefore, we cryopreserved the extracted premolars to prepare for autotransplantation in the lower first molar area because the tooth would probably need to be removed in the future. The teeth were frozen by a programmed freezer with a magnetic field (CAS freezer) that was developed for tissue cryopreservation and were cryopreserved in 2150uC deep freezer. After 1.5 years of presurgical orthodontic treatment, bilateral sagittal split ramus osteotomy was performed for mandible setback. Improvement of the facial profile and the occlusion were achieved in the retention phase. Six years after the initial visit, the patient had pain on the lower left first molar, and discharge of pus was observed, so we extracted the lower left first molar and autotransplanted the cryopreserved premolar. Three years later, healthy periodontium was observed at the autotransplanted tooth. This case report suggests that long-term cryopreservation of teeth by a CAS freezer is useful for later autotransplantation, and this can be a viable technique to replace missing teeth. (Angle Orthod. 2015;85:518-524.)

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“…이러한 세포 손상을 줄이기 위해 많은 방법들을 시험해 왔으며 대표적으로 동해방지제의 사용과 다양한 냉 동 방법의 개발을 들 수 있다. 최근 일본에서는 미소자장을 이용한 프로그램 냉동고 (Cell Alive System)를 이용하여 세포 핵내, 세포내, 세포 외 물분자를 자기장에 의해 진동시켜 결정화를 억제해 과냉 각 상태를 유지하다가 세포, 냉동 보존액 전체를 동시에 냉 동하게 하는 시도가 있었다 7,8) . 이 특수한 자장 냉동고를 사 용하여 Kawata 7) 는 쥐 치아를 15mA 자기장으로 -0.5℃ /min 속도로 -30℃까지 3일간 냉동 보관했을 때 급속 냉동 을 하는 것보다 좋은 결과를 얻었다 고 보고하였으며 Kaku 등 8) 은 사람의 치주인대세포를 75mA 자기장으로 -0.5℃ /min 속도로 -30℃까지 3일간 냉동 보관했을 때 세포활성 도가 제일 높았다고 보고하였다.…”

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The efficacy of programmed cryo-preservation under pressure in rat periodontal ligament cells

Lee

Kim

et al. 2009

J Korean Acad Conserv Dent

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The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group.Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at 4℃ for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37℃ water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization.In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group.By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.

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Cryopreservation of periodontal ligament cells with magnetic field for tooth banking

Cited by 84 publications

References 26 publications

Isolation of mesenchymal stromal/stem cells from cryopreserved umbilical cord blood cells

Isolation of mesenchymal stromal/stem cells from cryopreserved umbilical cord blood cells

A case of tooth autotransplantation after long-term cryopreservation using a programmed freezer with a magnetic field

The efficacy of programmed cryo-preservation under pressure in rat periodontal ligament cells

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