Cryopreservation of Persian sturgeon (Acipenser persicus) embryos by DMSO-based vitrificant solutions

Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (<i>Acipenser schrenckii</i>)

“…In the present study, after sperm cryopreservation, the MT+DMSO group obtained better effect in terms of the rate of hatched larval fish. The results is interesting and may be explained by a related report, which indicated that DMSO-based vitrificant solutions were suitable for cryopreservation of Persian sturgeon (A. persicus) embryos [10]. Moreover, a recent report indicated that the dramatically high level of malondialdehyde (MDA) across the embryonic development may be the main reason leading to a low hatching rate in A. schrenckii [19].…”

“…Although the frozen extenders based on methanol as cryoprotectant addition achieved the better survival rates of post-thaw sperm in many sturgeon species, the index of fertilization rate was remarkably unstable ( Table 1). The latest report indicated that cryoprotectants combination were suitable for cryopreservation of sturgeon embryos [10], which might suggest that cryoprotectants combination of sperm cryopreservation in sturgeon may be more suitable method. Therefore, the present study was conducted to test the effects of different cryoprotectants combination on sperm survival, fertility and subsequent embryo development in Amur sturgeon.…”

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“…In the present study, there were no differences in the results obtained using either 0 or 20°C thawing temperatures, this finding is in accordance with results obtained by Cabrita et al (2006) when studying gilthead seabream (Sparus aurata) embryos. Nevertheless, previous report (Keivanloo & Sudagar, 2016) showed better results using 20°C for thawing Persian sturgeon embryos. The present results cannot be compared directly with those of Keivanloo and Sudagar (2016), because there were several differences in the used methods, including substance that was used as basic cryoprotectant in vitrificant solutions, combinations of cryoprotectants and cryopreservation protocol.…”

“…Nevertheless, previous report (Keivanloo & Sudagar, 2016) showed better results using 20°C for thawing Persian sturgeon embryos. The present results cannot be compared directly with those of Keivanloo and Sudagar (2016), because there were several differences in the used methods, including substance that was used as basic cryoprotectant in vitrificant solutions, combinations of cryoprotectants and cryopreservation protocol.…”

“…Before the vitrificant solutions exposure, chorion permeabilization was performed using 2 mg/ml pronase at 20°C for 5 min (Keivanloo & Sudagar 2013, 2016. For incorporation of the vitrificant solutions, the embryos were exposed to a four-step protocol.…”