Cryopreservation of Piau‐Breed Wild Boar Sperm: Assessment of Cooling Curves and Centrifugation Regimes

Shiomi, HH; Pinho, Rogério Oliveira; Lima, D. M. A.; Siqueira, Jeanne Broch; Santos, Mcr; Costa, E.P.; Lopes, Paulo Sávio; Guimarães, S. E. F.; Guimarães, Mário

doi:10.1111/rda.12520

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…In boar sperm, it has been determined that freezing rates of either 20, 40, or 60 °C/min, which are achieved using automated freezing, resulted in the improvement of post-thaw motility (45 versus 52% for a conventional and automated freezing rate of 40 °C/min, respectively), viability, the acrosomal integrity of live sperm, and the mitochondrial membrane potential of live sperm compared to conventional slow freezing [ 11 ]. Different groups of researchers around the world use automated freezing systems and a temperature drop rate of −40 °C/min between −5 and −80 °C, which is when the freezing of the different cellular components occurs [ 12 , 13 , 14 ]. However, these systems are expensive, which means that many researchers and clinicians continue to perform the conventional freezing technique in a Styrofoam box, where there is no control of freezing rates, and there is also no standardization regarding the height where the straws are positioned and the time for which they are exposed to liquid nitrogen vapors [ 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Slow Freezing of Preserved Boar Sperm: Comparison of Conventional and Automated Techniques on Post-Thaw Functional Quality by a New Combination of Sperm Function Tests

Pezo,

Zambrano,

Uribe

et al. 2023

Animals

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The slow freezing of boar sperm is the only way to preserve genetic material for extended periods; this can be achieved with exposure to liquid nitrogen vapors (conventional) or by using automated freezing equipment. The aim was to compare the effect of both techniques on post-thaw functionality. Boar sperm devoid of seminal plasma and resuspended in lactose-egg yolk-glycerol medium were cryopreserved. Conventional: straws were exposed to LN2 vapors; automated: using a drop curve of −39.82 °C·min−1 for 113 s from −5 to −80 °C during the critical period; and subsequent immersion in NL2. Cell viability, cholesterol flow, mitochondrial membrane potential (MMP), lipid peroxidation, peroxynitrite, superoxide anion levels, phosphatidylserine translocation, and caspase activation were evaluated by flow cytometry. In addition, total motility (TM) and progressive motility (PM) were determined by the SCA system immediately (T0), 60 (T60), and 120 min (T120) post-thawing. Automated freezing significantly reduces cholesterol flow and free radical and lipid peroxidation levels, making it possible to preserve motility for 120 min of incubation. At the same time, viability, acrosome integrity, MMP, and caspase activation did not differ from the conventional technique. In conclusion, controlling the temperature drop curve using automated freezing equipment reduces oxidative/nitrosative stress, preserving membrane fluidity and sperm motility.

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Section: Introductionmentioning

confidence: 99%

Slow Freezing of Preserved Boar Sperm: Comparison of Conventional and Automated Techniques on Post-Thaw Functional Quality by a New Combination of Sperm Function Tests

Pezo,

Zambrano,

Uribe

et al. 2023

Animals

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The distribution and current state of biobanking in Indonesian wildlife: a systematic review

Sushadi¹

2023

Animal Conservation

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The Indonesian archipelago, where the tropical rainforest ecosystem is home to 15% of the world's vertebrate species, has been the hardest hit by deforestation in Southeast Asia for the last decade. This situation makes ex situ conservation strategies, particularly biobanks, more important than ever to supplement in situ efforts which are becoming increasingly difficult. This study examines the status and future of biobanking implementation in Indonesian wildlife. The results show that current biobanking efforts in Indonesian wildlife are still limited, with a non‐uniform distribution among taxa, sex and geographical origin. Current work is largely focused on mammals, while avifauna, reptiles and amphibians, particularly from the eastern region, are generally neglected. Cryopreservation is primarily used as a low‐cost preservation method, but success rates are strongly influenced by intra‐ and interspecific characteristics, making the development of species‐specific protocols essential. This report identifies the challenges and suggests ways to establish a national biobanking facility and collaborate with stakeholders to promote sustainable biobanking in Indonesia.

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Strong Heterogeneity in Advances in Cryopreservation Techniques in the Mammalian Orders

et al. 2018

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Between 1970 and 2012, vertebrate abundance has declined by 58% with an average annual decline of 2%, calling for serious action to prevent a mass extinction and an irreversible loss of biodiversity. Cryobanks and cryopreservation have the potential to assist and improve ex situ and in situ conservation strategies by storing valuable genetic material. A great deal of studies concerning cryopreservation have been performed within the class Mammalia, although no systematic overview has previously been presented. The objective of this study is therefore to evaluate the status, pattern and future of cryopreservation within Mammalia. A strong disproportional distribution of studies in examined orders is displayed. For the majority of examined orders less than 10% of species has been examined. However, the cryopreservation of germplasm has in several cases been successful and resulted in successful applications of assisted reproductive techniques (ARTs). Various obstacles are associated with the development of cryopreservation protocols, and among them the most prominent is interspecific differences in cryotolerance. Extrapolation of protocols in closely related species is considered the most applicable procedure, and a future supplement to overcome this problem is the examination and comparison of cryobiological traits. Successful protocols have been developed for the vast majority of domesticated mammals, which gives incentive for the further extrapolation of protocols in threatened species.

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Cryopreservation of Piau‐Breed Wild Boar Sperm: Assessment of Cooling Curves and Centrifugation Regimes

Cited by 3 publications

References 36 publications

Slow Freezing of Preserved Boar Sperm: Comparison of Conventional and Automated Techniques on Post-Thaw Functional Quality by a New Combination of Sperm Function Tests

Slow Freezing of Preserved Boar Sperm: Comparison of Conventional and Automated Techniques on Post-Thaw Functional Quality by a New Combination of Sperm Function Tests

The distribution and current state of biobanking in Indonesian wildlife: a systematic review

Strong Heterogeneity in Advances in Cryopreservation Techniques in the Mammalian Orders

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