Cryopreservation of platelets treated with riboflavin and <scp>UV</scp> light and stored at −80<scp>°C</scp> for 1 year

Jimenez‐Marco, Teresa; Ballester-Servera, Carme; Quetglas‐Oliver, Miguel; Morell-García, Daniel; Torres-Reverte, Natalia; Bautista-Gili, Antonia M.; Serra-Ramon, Neus; Girona-Llobera, Enrique

doi:10.1111/trf.16324

Cited by 7 publications

(10 citation statements)

References 48 publications

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“…11,14 One study reported TEG-MA values of 50-60 mm for products stored up to 6 months and ±40 mm for 12 month À80°C stored DTC. 43 Compared to published data the TEG-MA in our diluted samples was low (38-53 mm) but still higher compared to plasma and supernatant (25-29 mm). It has been shown previously that the 2000 G supernatant of DTC contain mostly platelet fragments and microparticles.…”

Section: Discussioncontrasting

confidence: 81%

“…Most studies use diluted samples with a platelet count of 200-300 Â 10 9 plt/L to study coagulation characteristics and TEG-MA of diluted DTC is 50-60 mm, 10,12,14,16,17,43 only a few report values below 50 mm. 11,14 One study reported TEG-MA values of 50-60 mm for products stored up to 6 months and ±40 mm for 12 month À80°C stored DTC.…”

Section: Discussionmentioning

confidence: 99%

“…Most studies use diluted samples with a platelet count of 200–300 × 10 9 plt/L to study coagulation characteristics and TEG‐MA of diluted DTC is 50–60 mm, 10,12,14,16,17,43 only a few report values below 50 mm 11,14 . One study reported TEG‐MA values of 50–60 mm for products stored up to 6 months and ±40 mm for 12 month −80°C stored DTC 43 . Compared to published data the TEG‐MA in our diluted samples was low (38–53 mm) but still higher compared to plasma and supernatant (25–29 mm).…”

Section: Discussionmentioning

confidence: 99%

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Frozen for combat: Quality of deep‐frozen thrombocytes, produced and used by The Netherlands Armed Forces 2001–2021

Noorman¹,

Rijnhout

Kort³

et al. 2022

Transfusion

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Background: The Netherlands Armed Forces (NLAF) are using À80°C deepfrozen thrombocyte concentrate (DTC) since 2001. The aim of this study is to investigate the effect of storage duration and alterations in production/ measurement techniques on DTC quality. It is expected that DTC quality is unaffected by storage duration and in compliance with the European guidelines for fresh and cryopreserved platelets. Study Design and Methods: Pre-freeze and post-thaw product platelet content and recovery were collected to analyze the effects of dimethyl sulfoxide (DMSO) type, duration of frozen storage (DMSO-1 max 12 years and DMSO-2 frozen DTC max 4 years at À80°C) and type of plasma used to suspend DTC. Coagulation characteristics of thawed DTC, plasma and supernatant of DTC (2Â 2500 G) were measured with Kaolin thromboelastography (TEG) and phospholipid (PPL) activity assay.Results: Platelet content and recovery of DTC is ±10%-15% lower in shortstored products and remained stable when stored beyond 0.5 years. Thawed DTC (n = 1724) were compliant to the European guidelines (98.1% post-thaw product recovery ≥50% from original product, 98.3% ≥200 Â 10 9 platelets/ unit). Compared to DMSO-1, products frozen with DMSO-2 showed ±8% reduced thaw-freeze recovery, a higher TEG clot strength (MA 58 [6] vs. 64 [8] mm) and same ±11 s PPL clotting time. The use of cold-stored thawed plasma instead of fresh thawed plasma did not influence product recovery or TEG-MA.

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Section: Discussioncontrasting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Frozen for combat: Quality of deep‐frozen thrombocytes, produced and used by The Netherlands Armed Forces 2001–2021

Noorman¹,

Rijnhout

Kort³

et al. 2022

Transfusion

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“…Tandem CPPs demonstrated faster mean R‐times compared with standard CPPs in both years, indicating a consistent and superior hemostatic quality for tandem CPPs over prolonged cryopreservation. For both CPP types, faster mean clotting times were observed in Y1 compared with Y2, although regardless of CPP type, year, and postthaw time points, mean R‐times observed were primarily under 4 min, and well within the two‐minute range even for tandem CPPs cryopreserved for up to 27 months at immediate postthaw, which are considerably faster R‐times among those reported irrespective of preparation methods, ranging from 3 to 11 min 20,26–34 . From two studies with longitudinal aspects that explored the time to clot initiation of thawed CPPs after 9 and 12 months of cryopreservation, 26,33 slight increments of R‐times have been observed over the first 6 months, but reduced after 9‐ and 12‐months respectively, hence a clear trend was not established.…”

Section: Discussionmentioning

confidence: 99%

Temporal dynamics of in vitro hemostatic function in platelets cryopreserved using a novel approach for rapid issuance

Kwan,

Kirwan,

Tuinukuafe

et al. 2024

Transfusion

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BackgroundCurrent procedures for thawing and issuing of cryopreserved platelets (CPPs) are laborious and have remained challenging in emergency settings such as blood banks and military operations. In this prospective study, a novel processing method designed to facilitate the rapid issuance of CPPs with no postthaw handling required was developed and functionally characterized in parallel with standard CPPs manufactured.Study Design and MethodsDouble‐dose plateletpheresis units (n = 42) were cryopreserved at −80°C in 5%–6% dimethyl sulfoxide to produce matched pairs thawed successively over a 27‐month period for comparison between two processing arms. In contrast to the standard CPPs manufactured as standalone units, platelets were frozen in tandem with resuspending plasma in a distinct partition as a single unit in the novel method, herein referred to as tandem CPPs. Postthaw (PT) CPPs from both arms were assessed at PT0‐, 12‐, and 24‐h to measure platelet recovery, R‐time (time to clot initiation; min), and maximum amplitude (MA; clot strength; mm) using thromboelastography.ResultsIn the overall dataset, mean platelet recovery was higher (p < .0005) for tandem CPPs (83.9%) compared with standard CPPs (73.3%) at PT0; mean R‐times were faster (p < .0005) for tandem CPPs (2.5–3.6 min) compared with standard CPPs (3.0–3.8 min); mean MA was higher for tandem CPPs (57.8–59.5 mm) compared with standard CPPs (52.1–55.8 mm) at each postthaw time point (p < .05).ConclusionRobust temporal dynamics of superior hemostatic functionality were established for tandem CPPs over extended cryopreservation up to 27 months and 24 h of postthaw storage.

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“…Some researchers have demonstrated the antioxidant function of riboflavin (Tang et al., 2014), and show that an altered riboflavin level causes spermatozoa morphological and bioenergetic defects in mice (Kuang et al., 2021). Riboflavin has recently been used to assist cryopreservation of platelets (Jimenez‐Marco et al., 2021). However, their functions in boar sperm are not well known.…”

Section: Discussionmentioning

confidence: 99%

Effects of riboflavin on boar sperm motility, sperm quality, enzyme activity and antioxidant status during cryopreservation

Dong

Luo

Liu

et al. 2022

Veterinary Medicine & Sci

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Objectives This study was conducted to evaluate the effect of adding riboflavin to boar sperm freezing extender on the challenge of cryopreservation. Methods Different concentrations (0, 5, 10, 15, 20 or 25 μM) of riboflavin were added to the freezing extender. Spermatozoa motility, membrane integrity, acrosomal integrity, mitochondrial membrane potential and enzyme activities were analysed once 10 min after thawing. Q‐PCR was used to detect the mRNA expression of Caspase3, Bcl‐2 and Bax. Results The results showed that the addition of 10 μM riboflavin to boar sperm freezing extender significantly increased the frozen‐thawed sperm progressive motility compared with the control group (p < 0.05). Activities of superoxide dismutase, glutathione peroxidase and catalase improved after adding riboflavin to the extender (p < 0.05). During freezing‐thawing, the boar sperm mitochondrial membrane potential, acrosomal integrity, plasma membrane and DNA at 10 μM in the riboflavin group increased by 6.6%, 9.6%, 5.49% and 5.62% (p < 0.05), respectively, compared with the control group. The addition of 10 μM riboflavin to the extender significantly decreased the malondialdehyde (p < 0.05) content, whereas it increased the ATP content (p < 0.05) of boar sperm during freezing‐thawing. Furthermore, the expression of Caspase‐3 and Bax (p < 0.05) were significantly lower, whereas the expression of BCL‐2 (p < 0.05) was greater than the control group when adding 10 μM riboflavin to the extender. Conclusions Riboflavin showed cryoprotective capacity to the freezing extender used for boar sperm during the process of freezing‐thawing, and the optimal concentration of riboflavin for the frozen extender was 10 μM.

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Cryopreservation of platelets treated with riboflavin and UV light and stored at −80°C for 1 year

Cited by 7 publications

References 48 publications

Frozen for combat: Quality of deep‐frozen thrombocytes, produced and used by The Netherlands Armed Forces 2001–2021

Frozen for combat: Quality of deep‐frozen thrombocytes, produced and used by The Netherlands Armed Forces 2001–2021

Temporal dynamics of in vitro hemostatic function in platelets cryopreserved using a novel approach for rapid issuance

Effects of riboflavin on boar sperm motility, sperm quality, enzyme activity and antioxidant status during cryopreservation

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