Cryopreservation of platelets using trehalose: The role of membrane phase behavior during freezing

Gläfke, Christiane; Akhoondi, Maryam; Oldenhof, Harriëtte; Sieme, Harald; Wolkers, Willem F.

doi:10.1002/btpr.1600

Cited by 46 publications

(43 citation statements)

References 63 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It should be noted, however, that the T g mostly reflects the extracellular matrix and not the intracellular T g . In a variety of studies we have shown that trehalose enters the cell during freezing-induced membrane phase transitions 31, 33, 34 . By contrast, the much larger albumin likely does not enter the cell during freezing-induced phase transitions.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity

Zhang

Oldenhof²,

Sydykov

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose alone or in a mixture of albumin and trehalose. Albumin was added to increase the glass transition temperature and storage stability. No viable cells were recovered after freeze-drying and rehydration. FTIR studies showed that membrane phase behavior of freeze-dried cells resembles that of fresh cells. However, one day after rehydration membrane phase separation was observed, irrespective of the presence or absence of trehalose during freeze-drying. Freeze-drying did not affect the overall protein secondary structure. Analysis of DNA damage via single cell gel electrophoresis (‘comet assay’) showed that DNA damage progressively increased with storage duration and temperature. DNA damage was prevented during storage at 4 °C. It is shown that trehalose reduces DNA damage during storage, whereas addition of albumin did not seem to have an additional protective effect on storage stability (i.e. DNA integrity) despite the fact that albumin increased the glass transition temperature. Taken together, DNA in freeze-dried somatic cells can be preserved using trehalose as protectant and storage at or below 4 °C.

show abstract

Section: Discussionmentioning

confidence: 99%

“…This occurs by membrane imperfections that are caused by freezing-induced membrane phase transitions 31, 32 . In a variety of studies, we have shown that freezing-induced trehalose uptake coincides with good cryosurvival of cells 31, 33, 34 .…”

Section: Introductionmentioning

confidence: 96%

Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity

Zhang

Oldenhof²,

Sydykov

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Beattie et al [3] made use of the leakiness associated with T m to introduce trehalose into the cells of pancreatic islets. It has also been shown that trehalose can enter red blood cells [14], and blood platelets [11,27] in significant concentrations when the temperature is lowered through Tm. The cell membrane of mammalian spermatozoa also undergoes a phase transition, associated with leakiness, as the temperature is lowered through T m [9,24].…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of human spermatozoa with minimal non-permeable cryoprotectant

et al. 2016

View full text Add to dashboard Cite

Cryopreservation of human spermatozoa is a commonly used technique in assisted reproduction, however freezing low concentrations of sperm while maintaining adequate post-thaw motility remains a challenge. In an effort to optimize post-thaw motility yields, low volumes of human sperm were frozen in polyimide-coated fused silica micro-capillaries using 0.065 M, 0.125 M, 0.25 M, or 0.5 M trehalose as the only cryoprotectant. Micro-capillaries were either initially incubated in liquid nitrogen vapor before plunging into liquid nitrogen, or directly plunged into liquid nitrogen. Post thaw sperm counts and motility were estimated. Spermatozoa that were initially incubated in liquid nitrogen vapor had greater post thaw motility than those plunged immediately into liquid nitrogen independent of trehalose concentration. The protective effect of 0.125 M D-glucose, 3-O-methyl-D-glucopyranose, trehalose, sucrose, raffinose, or stachyose were evaluated individually. Trehalose and sucrose were the most effective cryoprotectants, recovering 69.0% and 68.9% of initial sperm motility, respectively.

show abstract

“…On the contrary, membranes in platelets freeze-dried in the absence of sugar underwent a remarkable phase separation, with a pattern dissimilar from fresh platelets [77]. Also the uptake of trehalose has been suggested as a preservation mechanism, as in the case of RBCs [78].…”

Section: T I O Nmentioning

confidence: 99%

Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties

Cordone¹,

Cupane²,

Emanuele

et al. 2015

COC

View full text Add to dashboard Cite

Immobilization of proteins and other biomolecules in saccharide matrices leads to a series of peculiar properties that are relevant from the point of view of both biochemistry and biophysics, and have important implications on related fields such as food industry, pharmaceutics, and medicine. In the last years, the properties of biomolecules embedded into glassy matrices and/or highly concentrated solutions of saccharides have been thoroughly investigated, at the molecular level, through in vivo, in vitro, and in silico studies. These systems show an outstanding ability to protect biostructures against stress conditions; various mechanisms appear to be at the basis of such bioprotection, that in the case of some sugars (in particular trehalose) is peculiarly effective.Here we review recent results obtained in our and other laboratories on ternary protein-sugar-water systems that have been typically studied in wide ranges of water content and temperature. Data from a large set of complementary experimental techniques provide a consistent description of structural, dynamical and functional properties of these systems, from atomistic to thermodynamic level.In the emerging picture, the stabilizing effect induced on the encapsulated systems might be attributed to a strong biomolecule-matrix coupling, mediated by extended hydrogen-bond networks, whose specific properties are determined by the saccharide composition and structure, and depend on water content.

show abstract

Cryopreservation of platelets using trehalose: The role of membrane phase behavior during freezing

Cited by 46 publications

References 63 publications

Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity

Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity

Cryopreservation of human spermatozoa with minimal non-permeable cryoprotectant

Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties

Contact Info

Product

Resources

About