Cryopreservation of Porcine Gametes, Embryos and Genital Tissues: State of the Art

Rodriguez‐Martinez, Heriberto

doi:10.5772/34429

Cited by 5 publications

(6 citation statements)

References 153 publications

(131 reference statements)

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“…These differences in bio-physical events presented as different ice crystal formats in this study. Similar differences in ice matrix ultrastructure have been presented for sperm processed either in straws or bags [22] .…”

Section: Discussionsupporting

confidence: 71%

A scale down process for the development of large volume cryopreservation

et al. 2014

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The process of ice formation and propagation during cryopreservation impacts on the post-thaw outcome for a sample. Two processes, either network solidification or progressive solidification, can dominate the water–ice phase transition with network solidification typically present in small sample cryo-straws or cryo-vials. Progressive solidification is more often observed in larger volumes or environmental freezing. These different ice phase progressions could have a significant impact on cryopreservation in scale-up and larger volume cryo-banking protocols necessitating their study when considering cell therapy applications.This study determines the impact of these different processes on alginate encapsulated liver spheroids (ELS) as a model system during cryopreservation, and develops a method to replicate these differences in an economical manner.It was found in the current studies that progressive solidification resulted in fewer, but proportionally more viable cells 24 h post-thaw compared with network solidification. The differences between the groups diminished at later time points post-thaw as cells recovered the ability to undertake cell division, with no statistically significant differences seen by either 48 h or 72 h in recovery cultures.Thus progressive solidification itself should not prove a significant hurdle in the search for successful cryopreservation in large volumes. However, some small but significant differences were noted in total viable cell recoveries and functional assessments between samples cooled with either progressive or network solidification, and these require further investigation.

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Section: Discussionsupporting

confidence: 71%

A scale down process for the development of large volume cryopreservation

et al. 2014

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“…Thus, spermatozoa need to control water exchange with the extracellular fluids to adapt to different osmotic pressures (Chen and Duan 2011). Boar spermatozoa are well known for not surviving conventional cryopreservation well (Rodriguez-Martinez 2012). Due to high dehydration during the customary slow cooling and the quick rehydration during thawing, defective transmembranous water transport causes a substantial rate of membrane and organelle damage that leads to impairment of cell function, a reduced lifespan and, eventually, cell death (Ortman and Rodriguez-Martinez 1994).…”

Section: Discussionmentioning

confidence: 99%

Membrane Stress During Thawing Elicits Redistribution of Aquaporin 7 But Not of Aquaporin 9 in Boar Spermatozoa

Vicente-Carrillo

Ekwall

Álvarez‐Rodríguez

et al. 2016

Reprod Domestic Animals

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Freezing of boar spermatozoa includes the cryoprotectant glycerol, but renders low cryosurvival, owing to major changes in osmolarity during freezing/thawing. We hypothesize that aquaglyceroporins (AQPs)-7 and -9 adapt their membrane domain location to these osmotic challenges, thus maintaining sperm homeostasis. Western blotting (WB) and immunocytochemistry (ICC) at light-and electron microscope levels with several commercial primary antibodies explored AQP-location on cauda epididymal and ejaculated spermatozoa (from different fractions of the ejaculate); un-processed, extended, chilled and frozen-thawed.Although differences in WB-and ICC-labelling were seen among antibodies, AQP-7 was conspicuously located over the entire tail and cytoplasmic droplet in caudal spermatozoa, being restricted to the mid-piece and principal piece domains in ejaculated spermatozoa.AQP-9 was mainly localized over the sperm head both in caudal and ejaculated spermatozoa.While unaffected by chilling (+5ºC), freezing and thawing of ejaculated spermatozoa clearly relocated the head labelling of AQP-7-, but not that of AQP-9. In vitro mimicking of cell membrane expansion during quick thawing maintained the localization of AQP-9 but relocated AQP-7 towards the acrosome. AQP-7 but not AQP-9 appears as a relevant marker for non-empirical studies of sperm handling.

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“…This is not the case with other species. Boar spermatozoa in particular are very susceptible to cold shock [ 70 ], and as a result, the spermatozoa that undergo freezing are often weakened [ 72 ]. These weakened spermatozoa can suffer from DNA fragmentation, degradation of proteins and RNAs contained within the spermatozoa, disruption of the acrosome and sperm membranes, as well as reduction in mitochondrial activity and sperm motility [ 70 ].…”

Section: Semen Storagementioning

confidence: 99%

An Exploration of Current and Perspective Semen Analysis and Sperm Selection for Livestock Artificial Insemination

Zuidema

Kerns

Šutovský

2021

Animals

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Artificial insemination of livestock has been a staple technology for producers worldwide for over sixty years. This reproductive technology has allowed for the rapid improvement of livestock genetics, most notably in dairy cattle and pigs. This field has experienced continuous improvements over the last six decades. Though much work has been carried out to improve the efficiency of AI, there are still many areas which continue to experience improvement, including semen analysis procedures, sperm selection techniques, sperm sexing technologies, and semen storage methods. Additionally, the use of AI continues to grow in beef cattle, horses, and small ruminants as the technology continues to become more efficient and yield higher pregnancy rates. In this review, AI trends in the various livestock species as well as cutting edge improvements in the aforementioned areas will be discussed at length. Future work will continue to refine the protocols which are used for AI and continue to increase pregnancy rates within all livestock species.

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Cryopreservation of Porcine Gametes, Embryos and Genital Tissues: State of the Art

Cited by 5 publications

References 153 publications

A scale down process for the development of large volume cryopreservation

A scale down process for the development of large volume cryopreservation

Membrane Stress During Thawing Elicits Redistribution of Aquaporin 7 But Not of Aquaporin 9 in Boar Spermatozoa

An Exploration of Current and Perspective Semen Analysis and Sperm Selection for Livestock Artificial Insemination

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