Cryopreservation of Potato Cultivars - Design of a Method for Routine Application in Genebanks

Schäfer-Menuhr, Angelika; Schumacher, Heinz Martin; Mix-Wagner, Gunda

doi:10.17660/actahortic.1997.447.97

Cited by 38 publications

(30 citation statements)

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“…A few years after Bouman and De Klerk (1990) research, the vitrification method was successfully applied to five other lily genotypes (Matsumoto et al 1995), resulting in the first successful cryopreservation of lily. The dropletvitrification method is based on the droplet-freezing method developed for potato (Schäfer-Menuhr et al 1997). This method has also been successfully used for the cryopreservation of Prunus (De Boucaud et al 2002), Carica papaya (Ashmore et al 2001), Chrysanthemm (Halmagyi et al 2004), and Musa (Panis et al 2005).…”

Section: Effects Of Vitrification Solution and Application Time On Rementioning

confidence: 99%

Efficient Cryopreservation of Lilium spp. Shoot Tips using Droplet-vitrification

Lee

Chung

et al. 2013

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Newly developed shoot tips from adventitious buds induced by tissue cultured bulb-scale segments of five accessions of Lilium spp. were successfully cryopreserved by a droplet-vitrification method. Bulb-scale segments cultured on Murashige and Skoog (MS) medium with 0.1 mg·L -1 IAA and 0.1 mg·L -1 zeatin were then cold-hardened at 4℃ for 7 days. The excised shoot tips were pre-cultured on solidified MS medium containing 0.3 M sucrose for 1 day at 23℃ and then soaked in a mixture of 0.7 M sucrose for a day at 23℃. Pre-cultured shoot tips were cryoprotected with two loading solutions, LD1 and LD2, which included 35% and 40% plant vitrification solution (PVS3), respectively, for 40~60 min at 23℃. The cryoprotected shoot tips were then soaked in PVS2, modified PVS2 and PVS3 for 90~120 min at 23℃. The shoot tips, frozen in microdroplets of vitrification solution, were wrapped with aluminum foil strips, which were immersed rapidly in liquid nitrogen. The shoot tips were then rapidly warmed using unloading solution, transferred to a regeneration medium, stored in the dark for two weeks at 23℃, and then cultured under white fluorescent light at an intensity of 2000 lux with a 16-h photoperiod at 23℃. The average post-cryo regeneration rates of five accessions ranged from 52.7% to 87.5%.

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Section: Effects Of Vitrification Solution and Application Time On Rementioning

confidence: 99%

Efficient Cryopreservation of Lilium spp. Shoot Tips using Droplet-vitrification

Lee

Chung

et al. 2013

Plant Breed. Biotech.

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“…Vitrification has been applied to several types of medicinal plants with moderate to high recovery rates [13, 14]. The droplet vitrification technique was derived from the plant vitrification solution (PVS)-based vitrification technique [9] and the droplet freezing technique [15, 16]. These techniques involve the use of a sterile aluminum cryoplate [17], an important conductor of heat or thermal energy during immersion in LN [18, 19].…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Shoot Tips of Elite Cultivars of <b><i>Cannabis sativa</i></b> L. by Droplet Vitrification

Uchendu

Lata

Chandra

et al. 2019

Med Cannabis Cannabinoids

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Cannabis sativa L. (marijuana or hemp) is recognized worldwide for its psychoactive properties as well as for fiber production. This study focused on the evaluation of 3 droplet vitrification protocols for long-term conservation of shoot tips in liquid nitrogen (LN). Shoot tips (∼0.5 mm) were excised from 3- to 4-week-old in vitro-grown shoots of 3 cultivars (MX, VI-20, and B-5: high tetrahydrocannabinol [THC], high cannabidiol [CBD], and intermediate THC∼CBD, respectively) and pretreated on 5% dimethyl sulfoxide agar plates for 48 h. The shoot tips were then vitrified in LN using 3 separate cryoprotectant (plant vitrification solutions [PVS] #2, #3, and #4) droplets on an aluminum cryoplate. There was no significant difference between the regrowth of cryopreserved shoot tips exposed to PVS2 for 15 and 20 min, but regrowth of all 3 cultivars significantly declined after 20 min of exposure. Exposure duration of 15 min was adapted for subsequent experiments. Regrowth of cryopreserved MX was significantly higher with PVS2 (63%) than with PVS3 and PVS4 (≤5%). Regrowth of cryopreserved VI-20 was highest with PVS2 (57%) and significantly higher than with PVS3 and PVS4 (≤25%). The regrowth of cryopreserved shoot tips of B-5 was significantly different between all 3 protocols with PVS2 > PVS4 > PVS3. Both PVS2 and PVS4 produced regrowth above 55%, while regrowth with PVS3 was significantly lower (31%). These results indicate that 15–20 min of exposure to PVS2 are most suitable for cryopreservation of these varieties. This is the first report on protocol development for the cryopreservation of organized tissues of C. sativa L. for germplasm conservation.

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“…In another preculturing method given by Panis et al (1996), the proliferating meristems of banana were pre-cultured for 2-4 weeks on MS medium with 0.3-0.5M sucrose, then excised and plunged into LN. In the droplet freezing method by Schäfer et al (1997), apical shoot tips of potato were first incubated in DMSO, then transferred into 2.5ml droplets placed on small leaflets of aluminium foil and immersed in LN. All these techniques have been used for only a limited number of plant species.…”

Section: Other Protocolsmentioning

confidence: 99%

Fundamental Cryobiology and Basic Physical, Thermodynamical and Chemical Aspects of Plant Tissue Cryopreservation

Zeliang¹,

Pattanayak²

2012

Plant Breeding

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Cryopreservation of Potato Cultivars - Design of a Method for Routine Application in Genebanks

Cited by 38 publications

References 0 publications

Efficient Cryopreservation of Lilium spp. Shoot Tips using Droplet-vitrification

Efficient Cryopreservation of Lilium spp. Shoot Tips using Droplet-vitrification

Cryopreservation of Shoot Tips of Elite Cultivars of <b><i>Cannabis sativa</i></b> L. by Droplet Vitrification

Fundamental Cryobiology and Basic Physical, Thermodynamical and Chemical Aspects of Plant Tissue Cryopreservation

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