Cryopreservation of preantral ovarian follicles in situ from domestic cats (Felis catus) using different cryoprotective agents

Lima, Ana Kelen Felipe; Silva, Alexandre Rodrigues; Santos, Regiane R.; Sales, Daniele M.; Evangelista, Andreia F.; Figueiredo, José Ricardo de; Silva, Lúcia D.M.

doi:10.1016/j.theriogenology.2006.02.014

Cited by 26 publications

(23 citation statements)

References 8 publications

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“…Similar comparative studies were performed on ovarian tissue from humans [26], ovines [3], [4] and [24], caprines [22] and [25], bovines [5] and [16] and felines [14].…”

Section: Discussionmentioning

confidence: 70%

“…Me2SO and EG have been reported as the best cryoprotective agents for cryopreservation of ovarian tissue in many species (bovine [5] and [16], caprine [22] and [25] ovine [3], [4] and [24], humans [26] and feline [14]). When compared with each other, some authors found that EG was better than Me2SO for cryopreserving ovine, bovine and caprine ovarian tissue [3], [4], [5] and [25].…”

Section: Discussionmentioning

confidence: 99%

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Cryopreservation of swine ovarian tissue: Effect of different cryoprotectants on the structural preservation of preantral follicle oocytes

et al. 2009

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The present study aimed to test different cryoprotectants on cryopreservation of pig ovarian tissue. Pig ovaries (n = 3) were collected at a local slaughterhouse. From each ovary, ten cortex samples were taken. One was immediately fixed (control) and another placed in short-term tissue incubation (STTI control). The other 8 samples were cryopreserved, in pairs, using 4 different cryoprotectants: dimethyl sulphoxide (Me2SO -1.5 M), ethylene glycol (EG -1.5 M), propanediol (PROH -1.5 M) and glycerol (GLY -10%), all with 0.4% sucrose. Samples were slow cooled and stored in liquid nitrogen for 7 days. After thawing and cryoprotectant removal, one sample from each treatment was immediately fixed and the other was placed in short-term tissue incubation (STTI) for 2 h and then fixed. Samples were processed for histology and transmission electron microscopy. The percentages of morphologically normal follicles (MNF) in cryopreserved tissue using Me2SO (67.0 ± 4.9), EG (81.8 ± 1.4) and PROH (55.9 ± 9.9) were significantly lower (P < 0.05) than observed in fresh control tissue (97.7 ± 1.2). When ovarian tissue was cryopreserved with GLY, no morphologically normal follicles could be found (0%). After STTI, PROH showed a significantly lower percentage of MNF when compared with all other treatments and the control. After ultrastructural analysis, follicles cryopreserved with Me2SO and EG showed some small alterations, but no signs of advanced degeneration. Overall, these were similar to follicles from the control group. In conclusion, it is possible to cryopreserve preantral follicles from pig ovarian tissue using Me2SO or EG.

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“…Similar comparative studies were performed on ovarian tissue from humans [26], ovines [3], [4] and [24], caprines [22] and [25], bovines [5] and [16] and felines [14].…”

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Cryopreservation of swine ovarian tissue: Effect of different cryoprotectants on the structural preservation of preantral follicle oocytes

et al. 2009

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“…When associated with PROH, slow cooling in solid phase seems to be more appropriate. Nevertheless, several authors use cryopreservation protocols with a direct immersion into liquid nitrogen after -40°C, such as Rodrigues et al (2004) in the goat, Lucci et al (2004) in the zebu cow, in the ewe or Lima et al (2006) in the cat. Births had been obtained after graft of ovarian fragments frozen with such a protocol in the rabbit doe (Almodin et al, 2004b) and in the ewe (Almodin et al, 2004a), but not in the cat where in vivo follicular growth were observed when using a freezing protocol with controlled third phase (Bosch et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

New Approaches of Ovarian Tissue Cryopreservation from Domestic Animal Species

Neto¹,

Joly²,

Commin³

et al. 2012

Current Frontiers in Cryopreservation

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“…Intracellular cryoprotectant agents (CPAs) have been investigated for use in these solutions. Such agents include ethylene glycol (EG; feline: Lima et al 2006;caprine: Faustino et al 2010), propanediol (PROH; bovine: Lucci et al 2004;caprine: Luz et al 2009), and dimethyl sulfoxide (DMSO; ovine: Pinto et al 2008;caprine: Luz et al 2009). However, no studies have compared, under optimal conditions, the efficiency of these agents for the preservation of goat ovarian tissue.…”

Section: Introductionmentioning

confidence: 99%

Freezing solution containing dimethylsulfoxide and fetal calf serum maintains survival and ultrastructure of goat preantral follicles after cryopreservation and in vitro culture of ovarian tissue

et al. 2011

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Goat ovarian cortex fragments were subjected to slow freezing in the presence of various solutions containing intracellular cryoprotectants, including 1.0 M ethylene glycol (EG), propanediol (PROH), or dimethyl sulfoxide (DMSO), with or without sucrose and/or fetal calf serum (FCS). Histological examination revealed that only the DMSO-containing solutions were able to maintain a follicular ultrastructure similar to the morphology observed in the fresh control. Therefore, fragments previously cryopreserved in DMSO solutions (with and without sucrose and/or FCS) were cultured in vitro for 48 h and then subjected to viability, histological, and ultrastructural analysis. No significant differences were observed among the percentages of morphologically normal follicles in cryopreserved ovarian tissue before in vitro culture (DMSO: 62.5%; DMSO + sucrose: 68.3%; DMSO + FCS: 60.0%; DMSO + sucrose + FCS: 60.0%) and after culture (DMSO: 60.8%; DMSO + sucrose: 64.2%; DMSO + FCS: 70.8%; DMSO + sucrose + FCS: 55.0%). Following in vitro culture, the viability analysis showed that only the freezing solution containing DMSO and FCS (75.6%) maintained a percentage of viable follicles similar to that observed after culture without cryopreservation (89.3%). As determined by ultrastructural analysis, morphologically normal preantral follicles were detected in the fresh control and in fragments cultured before and after cryopreservation with DMSO and FCS. Thus, a freezing solution containing DMSO and FCS, under the experimental conditions tested here, guaranteed the maintenance of viability and follicular ultrastructure after short-term in vitro culture.

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Cryopreservation of preantral ovarian follicles in situ from domestic cats (Felis catus) using different cryoprotective agents

Cited by 26 publications

References 8 publications

Cryopreservation of swine ovarian tissue: Effect of different cryoprotectants on the structural preservation of preantral follicle oocytes

Cryopreservation of swine ovarian tissue: Effect of different cryoprotectants on the structural preservation of preantral follicle oocytes

New Approaches of Ovarian Tissue Cryopreservation from Domestic Animal Species

Freezing solution containing dimethylsulfoxide and fetal calf serum maintains survival and ultrastructure of goat preantral follicles after cryopreservation and in vitro culture of ovarian tissue

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