Cryopreservation of primed tobacco seeds

Lopes, Camila Aparecida; Carvalho, María Laene Moreira de; Souza, Ana Cristina de; Oliveira, João Almir; Andrade, Dayliane Bernardes de

doi:10.1590/2317-1545v40n4207216

Cited by 5 publications

(7 citation statements)

References 14 publications

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“…Seeds were harvested on plants before pod shattering and removed from fruits, after that, were stored in cold chamber for about 20 days, when the experiments were started. Priming consisted in five solutions: distilled water, 0.2% potassium nitrate (Cardoso et al, 2015), 0.5 mmol.L -1 spermidine (SPE) (Hussain et al, 2015;Lopes et al, 2018), 50 mg.L -1 gibberellin (GA) (Batista et al, 2015;Cardoso et al, 2015), and 0.10 mmol.L -1 sodium nitroprusside (SNP) (Faraji and Sepehri, 2018). Treatments consisted in immersion of 40 g seeds in 400 mL of above-described solutions in Erlenmeyer, being the solution kept aerated with the support of an air compressor.…”

Section: Methodsmentioning

confidence: 99%

Priming Urochloa ruziziensis (R.Germ. & Evrard) seeds with signalling molecules improves germination

et al. 2022

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As forage production increase, high-quality seeds demand follows. Priming have been reported as a technique for improve seed quality and stress tolerance. The aim of this study was to evaluate the priming agents effect on Urochloa ruziziensis seed germination and seedling establishment under water deficit and salt stress. A completely randomized experiment assessing six priming agents and three germination conditions were established. We evaluated: seed water content; germination percentage and speed index; viability of seeds remaining from the germination test; seedling length; antioxidant enzymatic activity; and malondialdehyde quantity. Seeds primed under water deficit and salt stress showed better physiological performance than dry seeds. There was a significant increase in seed germination percentage and speed and in seedling roots and shoots length. Sodium Nitroprusside shows significant potential for use in the physiological priming of U. ruziziensis seeds.

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Section: Methodsmentioning

confidence: 99%

Priming Urochloa ruziziensis (R.Germ. & Evrard) seeds with signalling molecules improves germination

et al. 2022

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“…Each treatment was primed in a single Erlenmeyer flask, without replications. The priming solutions used were distilled water (water), 0.2% potassium nitrate solution (KNO 3 ) (Cardoso et al, 2014;Cardoso et al, 2015), 0.5 mmol.L −1 spermidine solution (SPER) (Hussain et al, 2015;Lopes et al, 2018), 50 mg.L −1 pure gibberellin solution (GB) (Batista et al, 2015;Cardoso et al, 2015), and 0.10 mmol.L −1 sodium nitroprusside solution (SNP) (Ataíde et al, 2015;Silva et al, 2015;Faraji et al, 2018). The seeds used as a control were not primed.…”

Section: Methodsmentioning

confidence: 99%

“…Various authors have found that the positive effects of the SPER are associated with maintenance of membrane integrity, regulation of gene expression, breaking of dormancy, and seed germination, flower and fruit development, and senescence (Gupta et al, 2014;Lopes et al, 2018;Yalamalle et al, 2019). In addition, the SPER also plays a crucial role in tolerance to abiotic stresses (Yalamalle et al, 2019).…”

Section: Journal Of Seedmentioning

confidence: 99%

Protective action of priming agents on Urochloa brizantha seeds under water restriction and salinity conditions

et al. 2021

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Analyses of germination of seeds under artificial stress conditions are tools for a better understanding of the survival and adaptation capacity of species under natural stress conditions. Therefore, the aim of this study was to evaluate the effect of priming on seed germination, on breaking of dormancy, and on the length of Urochloa brizantha seedlings under water restriction and salt stress conditions. A completely randomized experimental design was used with four replications in a 3 × 6 factorial arrangement consisting of 3 germination conditions (water, water restriction with PEG, and salinity with NaCl) and 6 treatments [five priming solutions (distilled water, potassium nitrate, spermidine, gibberellin, and sodium nitroprusside) and a control (unprimed seeds)]. The following determinations were made: seed moisture content after priming (before drying) and after drying the seeds, germination test at seven and twenty-one days after sowing, germination speed index, viability by the tetrazolium test of the seeds remaining from the germination test, and seedling length. Priming is effective in minimizing the negative effects of water restriction and salinity. Use of sodium nitroprusside as a priming solution results in a lower percentage of dormant seeds and greater germination, vigor, and plant development.

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“…Therefore, it also has the potential to be applied to forage grass seeds, helping to increase the speed and uniformity of seed germination with positive impacts on pasture formation (Batista et al, 2016). Osmopriming can be performed using different osmotic solutions, such as spermidine (Lopes et al, 2018), gibberellin (Li et al, 2019), sodium nitroprusside (SNP) (Kaiser et al, 2016), polyethylene glycol (PEG-6000) (Lei et al, 2021), and sodium selenate (Silveira et al, 2023). Oliveira et al (2021) evaluated the effect of osmopriming with SNP in U. brizantha seeds and found an improvement in the physiological performance of seeds under water and saline stress conditions.…”

Section: Introductionmentioning

confidence: 99%

OSMOPRIMING AND ITS RELATIONSHIP WITH THE QUALITY OF Urochloa decumbens SEEDS UNDER WATER DEFICIT

Peris,

Pinheiro,

Limão

et al. 2024

Rev. Agric. Neotrop.

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The use of high-quality Urochloa decumbens seeds is an essential factor for the establishment of pastures, and osmopriming under stress conditions may improve seed performance. This study was conducted at the Universidade Federal de Viçosa in a completely randomized design and aimed to evaluate the effects of osmopriming and its relationship with physical and physiological attributes of U. decumbens seeds subjected to water deficit. Seeds from ten lots were primed in H2O (0 MPa) and PEG (−0.8 MPa), KNO3 (0.2%), and SNP (0.10 mmol L−1) solutions for 24 hours. Unprimed seeds were used as control. The seeds were evaluated in two trials after osmopriming. Trial I consisted of the X-ray test to evaluate physical attributes and germination and vigor tests. In Trial II, the seeds were placed to germinate under favorable (0 MPa) and water deficit conditions (−0.2 MPa) to evaluate physiological attributes. In general, osmopriming of U. decumbens seeds assisted in increasing physical attributes such as area, gray scale, perimeter, and tissue density. Osmopriming does not contribute to the best physiological performance of U. decumbens seeds under ideal germination conditions (0 MPa). However, osmopriming with SNP (0.10 mmol L−1) and PEG (−0.8 MPa) for 24 hours contributes to the better physiological performance of seeds under water deficit.

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Cryopreservation of primed tobacco seeds

Cited by 5 publications

References 14 publications

Priming Urochloa ruziziensis (R.Germ. & Evrard) seeds with signalling molecules improves germination

Priming Urochloa ruziziensis (R.Germ. & Evrard) seeds with signalling molecules improves germination

Protective action of priming agents on Urochloa brizantha seeds under water restriction and salinity conditions

OSMOPRIMING AND ITS RELATIONSHIP WITH THE QUALITY OF Urochloa decumbens SEEDS UNDER WATER DEFICIT

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