Cryopreservation of Prunus

Boucaud, Marie‐Thérèse de; Brison, M.; Helliot, B.; Hervé-Paulus, Valérie

doi:10.1007/978-3-662-04674-6_21

Cited by 13 publications

(11 citation statements)

References 15 publications

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“…For potato shoot tips a cryoprotectant solution containing 10% dimethylsulphoxide (DMSO) was used, while for these experiments the classical PVS2 (Sakai et al, 1990) solution was applied. The combined droplet vitrification method was successfully used for cryopreservation of Prunus (De Boucaud et al, 2002), Carica papaya (Ashmore et al, 2001), Chrysanthemum (Halmagyi et al, 2004) or Musa (Panis et al, 2005). A comparison between the droplet vitrification and the cryovial vitrification method showed higher recovery rates for Musa shoot tips after droplet vitrification (Panis et al, 2005).…”

Section: Introductionmentioning

confidence: 97%

“…The vitrification procedure offers a great potential for complex tissues e.g. shoot tips (Steponkus et al, 1992;Thinh et al, 1999) and was used for cryopreservation of sweet potato (Pennycooke and Towill, 2000), mint (Towill and Bonnart, 2003) or Prunus (De Boucaud et al, 2002). The droplet method was established for cryopreservation of potato (Scha¨fer-Menuhr et al, 1997), Asparagus (Mix-Wagner et al, 2000), Chrysanthemum (Halmagyi et al, 2004) or yam (Leunufna and Keller, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Plant regeneration from Rosa shoot tips cryopreserved by a combined droplet vitrification method

Halmágyi

Pinker

2006

Plant Cell Tiss Organ Cult

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Shoot tips obtained from in vitro Rosa plants (three cultivars) were successfully cryopreserved by a combined droplet vitrification method and subsequently shoots regenerated. The excised shoot tips (1-4 mm long) were incubated in a liquid MS medium supplemented with 2.5 mg l )1 thiamine, 0.2 mg l )1 biotin, 0.2 mg l )1 pyridoxine, 0.25 mg l )1 6-benzylaminopurine (BAP), 0.5 mg l )1 gibberellic acid (GA 3 ) and 0.08 M sucrose, for 24 h. Following that incubation shoot tips were pre-cultured in this MS medium containing 0.1 till 1.0 M sucrose for 24 and 48 h, respectively. Pre-cultured shoot tips were dehydrated with concentrated PVS2 cryoprotective solution for 10-30 min at room temperature, prior to a direct plunge in liquid nitrogen. After rapid rewarming in the above mentioned liquid medium shoot tips were plated on a modified MS medium (5 g l )1 agar) supplemented with vitamins and plant growth regulators as mentioned above for regrowth. Cryopreserved shoot tips resumed growth within 10 days and regenerated shoots within 3 weeks. The highest numbers of regrowing shoot tips were 64.44% for cv. Kardinal, 67.73% for cv. Fairy and 57.57% for cv. Maidy.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Plant regeneration from Rosa shoot tips cryopreserved by a combined droplet vitrification method

Halmágyi

Pinker

2006

Plant Cell Tiss Organ Cult

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“…The dropletvitrification method is based on the droplet-freezing method developed for potato (Schäfer-Menuhr et al 1997). This method has also been successfully used for the cryopreservation of Prunus (De Boucaud et al 2002), Carica papaya (Ashmore et al 2001), Chrysanthemm (Halmagyi et al 2004), and Musa (Panis et al 2005). Chen et al (2011) reported the first successful cryopreservation of in vitro-grown apical meristems of Lilium by a dropletvitrification method.…”

Section: Effects Of Vitrification Solution and Application Time On Rementioning

confidence: 99%

Efficient Cryopreservation of Lilium spp. Shoot Tips using Droplet-vitrification

Lee

Chung

et al. 2013

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Newly developed shoot tips from adventitious buds induced by tissue cultured bulb-scale segments of five accessions of Lilium spp. were successfully cryopreserved by a droplet-vitrification method. Bulb-scale segments cultured on Murashige and Skoog (MS) medium with 0.1 mg·L -1 IAA and 0.1 mg·L -1 zeatin were then cold-hardened at 4℃ for 7 days. The excised shoot tips were pre-cultured on solidified MS medium containing 0.3 M sucrose for 1 day at 23℃ and then soaked in a mixture of 0.7 M sucrose for a day at 23℃. Pre-cultured shoot tips were cryoprotected with two loading solutions, LD1 and LD2, which included 35% and 40% plant vitrification solution (PVS3), respectively, for 40~60 min at 23℃. The cryoprotected shoot tips were then soaked in PVS2, modified PVS2 and PVS3 for 90~120 min at 23℃. The shoot tips, frozen in microdroplets of vitrification solution, were wrapped with aluminum foil strips, which were immersed rapidly in liquid nitrogen. The shoot tips were then rapidly warmed using unloading solution, transferred to a regeneration medium, stored in the dark for two weeks at 23℃, and then cultured under white fluorescent light at an intensity of 2000 lux with a 16-h photoperiod at 23℃. The average post-cryo regeneration rates of five accessions ranged from 52.7% to 87.5%.

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“…High cooling and thawing rates appear to significantly increase the probability of obtaining a vitrified state during freezing and of avoiding devitrification during warming (Panis et al 2005). Droplet-vitrification using PVS2 has been successfully adapted to a range of plant materials (Sakai and Engelmann 2007) including meristems of banana (Agrawal et al 2004), yam (Leunufa and Keller 2003), almond, and plum (de Boucaud et al 2002); however, vanilla tissues proved to be very sensitive to PVS2 and were either killed (apices derived from clusters of in vitro plantlets obtained from microcuttings) or damaged before cooling (apices derived from clusters of in vitro plantlets obtained from IFEs), whatever the preconditioning and loading treatments tested. Contrarily to what was observed with apices of other orchid species (Sakai 2004), vanilla apices could not be cryopreserved using the vitrification method.…”

Section: Discussionmentioning

confidence: 97%

Multiplication and cryopreservation of vanilla (Vanilla planifolia ‘Andrews’)

Gonzalez-Arnao

Lazaro-Vallejo

Engelmann

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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A simple and efficient method for multiplication of vanilla (Vanilla planifolia) was developed using in vitro fragmented explants (IFEs) as propagules. IFEs were obtained after dissecting apices from in vitro propagated clusters of plantlets, by cutting the remaining base of these plant clusters into segments of about 1 cm in length. After 4 months of culture on multiplication medium, 100% of IFEs produced up to 15 new shoots per explant, providing an efficient additional method for in vitro propagation of vanilla that maximizes the use of available material. Cryopreservation of apices from in vitro grown plants was achieved using the droplet vitrification protocol. Maximum survival (30%) and further regeneration (10%) of new shoots were obtained for apices derived from clusters of in vitro plantlets produced from microcuttings through a three-step droplet vitrification protocol: 1-d preculture of apices on solid MS medium with 0.3 M sucrose; loading with a 0.4 M sucrose + 2 M glycerol solution for 20-30 min; and exposure to plant vitrification solution PVS3 for 30 min at room temperature. Even though the cryogenic protocol needs to be optimized to improve results, this work represents the first successful report of cryopreservation of vanilla apices.

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Cryopreservation of Prunus

Cited by 13 publications

References 15 publications

Plant regeneration from Rosa shoot tips cryopreserved by a combined droplet vitrification method

Plant regeneration from Rosa shoot tips cryopreserved by a combined droplet vitrification method

Efficient Cryopreservation of Lilium spp. Shoot Tips using Droplet-vitrification

Multiplication and cryopreservation of vanilla (Vanilla planifolia ‘Andrews’)

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