Cryopreservation of Robinia pseudoacacia

Verleysen, Hans; Fernandes, Pedro Alexandrino; Pinto, I. Sánchez; Bockstaele, Erik Van; Debergh, Pierre

doi:10.1007/s11240-004-4957-x

Cited by 11 publications

(2 citation statements)

References 26 publications

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“…Shoot tips of American chestnut were precultured stepwise for 96 h in sucrose-enriched solution with concentrations ranging from 0.25 to 1.0 M, which significantly shortened the preculture duration. The progressive increase of sucrose concentration during preculture treatments was found to improve the survival and regeneration of numerous species such as Corrigin grevillea [ 98 ], sugar beet [ 99 ], black locust [ 100 ], potato [ 101 ], and grapevine [ 102 ]. Jorquera et al [ 44 ] indicated that shoot tips of European chestnut provided a sufficient recovery rate when precultured in sucrose concentrations between 0.1 to 0.4 M for two days at 4 °C, while shoot tips precultured in higher sucrose concentration (0.7 M) showed a significantly decreased recovery (7.4%).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Technologies for American Chestnut (Castanea dentata (Marshall) Borkh) Conservation

Liu

Shukla

et al. 2022

Plants

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American chestnut (Castanea dentata), a native species of eastern North America, is an economically important deciduous hardwood tree that has been designated as endangered in Canada. The population of American chestnut trees has dwindled significantly across Southern Ontario due to chestnut blight and many of the surviving trees continue to show blight disease symptoms. American chestnut requires efficient strategies for propagation and preservation for species recovery. The objective of this study was to develop a long-term plant conservation program using micropropagation and cryopreservation protocols. An in vitro technology using a liquid-based temporary immersion system (TIS) was developed for micropropagation of American chestnut. The highest rate of shoot multiplication was observed in cultures grown in the DKW (Driver and Kuniyuki 1984) basal medium supplemented with 2.2 µM 6-benzylaminopurine and 1.0 µM gibberellic acid. More than 95% of proliferated microshoots, about 40–50 mm in size, developed roots after 30 days of culture within bioreactor vessels containing DKW basal medium supplemented with 15 µM 3-Indolebutyric acid. Rooted plantlets transplanted to the greenhouse had a survival efficiency of 82% after one month of growth. The cryopreservation protocol for germplasm preservation was developed through droplet vitrification of shoots. Optimal regeneration of shoot tips occurred from explants precultured on stepwise concentrations of sucrose and subsequent dehydration in PVS3 for 30 min. Cryopreserved shoot tips were regenerated to whole plants using pre-optimized conditions of micropropagation. This study confirms the potential of TIS for micropropagation in ex situ conservation and reintroduction of endangered American chestnuts and possibly other woody plant species.

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Section: Discussionmentioning

confidence: 99%

In Vitro Technologies for American Chestnut (Castanea dentata (Marshall) Borkh) Conservation

Liu

Shukla

et al. 2022

Plants

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“…Somatic seedlings were regenerated from L. tulipifera cultures stored in liquid nitrogen for 48 h (Vendrame et al 2001). Verleysen et al (2005) reported the successful cryopreservation of R. pseudoacacia via vitrification and encapsulation-dehydration. Seeds of T. cordata show 65-75% seedling emergence after freezing in liquid nitrogen, if seeds are dried to 11-20% moisture content and scarified before freezing (Chmielarz 2002).…”

Section: In Vitro Culturementioning

confidence: 99%

Technological advances in temperate hardwood tree improvement including breeding and molecular marker applications

Pijut

Woeste

Vengadesan

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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Hardwood forests and plantations are an important economic resource for the forest products industry worldwide and to the international trade of lumber and logs. Hardwood trees are also planted for ecological reasons, for example, wildlife habitat, native woodland restoration, and riparian buffers. The demand for quality hardwood from tree plantations will continue to rise as the worldwide consumption of forest products increases. Tree improvement of temperate hardwoods has lagged behind that of coniferous species and hardwoods of the genera Populus and Eucalyptus. The development of marker systems has become an almost necessary complement to the classical breeding and improvement of hardwood tree populations for superior growth, form, and timber characteristics. Molecular markers are especially valuable for determining the reproductive biology and population structure of natural forests and plantations, and the identity of genes affecting quantitative traits. Clonal reproduction of commercially important hardwood tree species provides improved planting stock for use in progeny testing and production forestry. Development of in vitro and conventional vegetative propagation methods allows mass production of clones of mature, elite genotypes or genetically improved genotypes.Genetic modification of hardwood tree species could potentially produce trees with herbicide tolerance, disease and pest resistance, improved wood quality, and reproductive manipulations for commercial plantations. This review concentrates on recent advances in conventional breeding and selection, molecular marker application, in vitro culture, and genetic transformation, and discusses the future challenges and opportunities for valuable temperate (or "fine") hardwood tree improvement.

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Black Locust

Arrillaga

Segura

Merkle

2008

Compendium of Transgenic Crop Plants

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Black locust ( Robinia pseudoacacia L.), a multipurpose nitrogen‐fixing leguminous forest tree, is among the most widely planted tree species in the world. In this review, available protocols for genetic transformation of the species are updated. The review describes both direct and Agrobacterium ‐mediated methods for gene transfer using different explants, bacterial strains, and plasmids and selection agents. Regeneration methods and tests of transgene integration are also covered. Although phenotypically normal transgenic black locust plants have been obtained via Agrobacterium tumefaciens ‐mediated transformation, there are few reports describing engineering of the species with genes of potential economic importance. Thus the transformation challenge for the near future in black locust must lie in extending the established protocols to genes of interest such as those related to pathogen and pest resistance, yield improvement, manipulation of wood formation and phytoremediation.

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Cryopreservation of Robinia pseudoacacia

Cited by 11 publications

References 26 publications

In Vitro Technologies for American Chestnut (Castanea dentata (Marshall) Borkh) Conservation

In Vitro Technologies for American Chestnut (Castanea dentata (Marshall) Borkh) Conservation

Technological advances in temperate hardwood tree improvement including breeding and molecular marker applications

Black Locust

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