Cryopreservation of Seabream (Sparus aurata) Spermatozoa

Fabbrocini, Adele; Lavadera, Silvestro Lubrano; Rispoli, Sabina; Sansone, Giovanni

doi:10.1006/cryo.1999.2220

Cited by 72 publications

(40 citation statements)

References 14 publications

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“…Since the first successful cryopreservation of herring sperm 50 years ago (Blaxter, 1953) considerable improvement has been achieved in sperm cryopreservation and developed technology of conventional freezing of fish spermatozoa has been used in agricultural practice very broadly (Scott and Baynes, 1980;Stoss and Holtz, 1981;Dreanno et al, 1997;Wheeler and Thorgaard, 1991;Conget et al, 1996;Lahnsteiner et at., 2000;Fabbrocini et al, 2000;Zhang et al, 2003;Chen et al, 2004;Viveiros and Godinho, 2009). Usually, for protection of spermatozoa from the negative effects of low temperatures caused by conventional freezing ('slow', with controlled rate of cooling), permeable cryoprotectants are used.…”

Section: Wwwintechopencommentioning

confidence: 99%

Vitrification Technique - New Possibilities for Male Gamete Low-Temperature Storage

Isachenko¹,

Mallmann²,

Rahimi³

et al. 2012

Current Frontiers in Cryobiology

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Section: Wwwintechopencommentioning

confidence: 99%

Vitrification Technique - New Possibilities for Male Gamete Low-Temperature Storage

Isachenko¹,

Mallmann²,

Rahimi³

et al. 2012

Current Frontiers in Cryobiology

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“…In this study, seabreams of 2-3 years old and weighing 900-1300 g were used, whereas Barbato et al (16) used seabreams of approximately 1 year old weighing 118-444 g. Therefore, the difference in amounts of collected semen may be explained by the use of seabreams of different ages and weights.…”

Section: Discussionmentioning

confidence: 98%

Cryopreservation of seabream (Sparus aurata) semen and fertility

Tirpan¹,

Özgöray²,

Alemdar³

et al. 2016

Turk J Vet Anim Sci

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IntroductionReproduction in fish is under the influence of various external factors. The most important factors are photoperiod, water temperature, water quality (dissolved oxygen, pH, hardness, salinity, alkalinity), floods, water currents, tides and cycles of the moon, weather conditions (atmospheric pressure, rainfall), spawning substrates (aquatic plants, pieces of wood, wards), nutrition, diseases, parasites, and the presence of other fish (1). In natural conditions, these fish are mostly female after 2 years of age (2). Seabream (Sparus aurata) has reproductive properties such as protandrous hermaphroditism, asynchronous ovarian development, and multiple daily spawnings (3,4).Newly spawned seabream eggs are approximately 0.9-1 mm in diameter and are transparent. Normally they contain one single oil drop and have a pelagic structure. Since the eggs have a pelagic structure, only the eggs that can float in water are viable and can form embryos (5,6). The egg membrane is transparent and thin, and the micropile hole is about 14 µm (2,7). After the seabream eggs complete their maturation and they are left in water and hydrolyzed, the egg diameter can be up to 1100 µm (8).Since fish spermatozoa are not motile when they leave the testes, they should be activated in species-specific ways (fresh water or sea fish species) (9). For spermatozoa activation, a number of extracellular agents were reported. Flagellar activation is provided by hyperosmotic-osmotic (500-1100 mOsm) medium in sea fish and by hypoosmotic (0-100 mOsm) medium in freshwater fish. In salmonids and sturgeon the most important motility activation factor is potassium (K + ) concentration (10,11). It was stated that in seabream K + and calcium (Ca 2+ ) had no effect on spermatozoa activation and that the optimal osmolarity for this activation was 1100 mOsm/kg (11).Penetration happens quickly after extending because fish spermatozoa are very small in structure; thus, an equilibration step is not required. The toxic effects of cryoprotectants can be minimized by not applying the equilibration stage. In a study conducted on seabream, when dimethyl sulfoxide (DMSO)-added semen was left for equilibration for more than 2 min, fertilization rates were lower (12,13).Fish semen is not appropriate for freezing without extending. The extenders used for freezing sea fish semen are mostly salty (1%-10% concentration) or sugary

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“…Dimethyl sulfoxide is the most widely used cryoprotectant in cryopreservation of fish sperm and provides better protection at a concentration of 5% to 25% in many marine fish species [18,21,22]. Besides DMSO, EG and PG are frequently used as cryoprotectant because of their rapid penetration into the cell membranes and low toxicity [18,23].…”

Section: Discussionmentioning

confidence: 99%

Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)

Liu

et al. 2015

Theriogenology

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Cryopreservation of Seabream (Sparus aurata) Spermatozoa

Cited by 72 publications

References 14 publications

Vitrification Technique - New Possibilities for Male Gamete Low-Temperature Storage

Vitrification Technique - New Possibilities for Male Gamete Low-Temperature Storage

Cryopreservation of seabream (Sparus aurata) semen and fertility

Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus)

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