Cryopreservation of seeds from endangered Brazilian bromeliads - Dyckia brevifolia Baker and D. delicata Larocca &amp; Sobral

Paula, Jean Carlo Baudraz de; Men, Gabriel Barraca; Biz, Guilherme; Júnior, Walter Aparecido Ribeiro; Faria, Ricardo Tadeu de

doi:10.5039/agraria.v15i4a8439

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…In liquid nitrogen storage (-196 °C), all metabolic activity and cell divisions are interrupted, preventing the degradation of the stored material and contamination (Cruz -Cruz et al 2013). Direct immersion in liquid nitrogen has the advantage of not requiring the use of cryoprotectants in materials with tolerance to cooling and low moisture content, as seen in the present study, and with several examples, such as the one seen for Dyckia brevifolia Beker and Dyckia delicata Larocca & Sobral seeds (De Paula et al 2020), mature seeds of Cattleya guttata Lindl (Vettorazzi et al 2019) and for Passiflora edulis Sims seeds (Generoso et al 2019). Although the present study hasn't observed a difference between the storage treatments, it is assumed that cryopreservation has more long-term advantages when compared to storage in a refrigerator, in which there may be degradation and contamination of the material in the medium term.…”

Section: Discussionsupporting

confidence: 53%

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Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest

Montoya-Serrano

Vesco

Pescador

2022

Hoehnea

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Vriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this research was to evaluate the cryopreservation using the method of direct immersion of its seeds, collected from capsules at 120, 135, and 150 days after anthesis (DAA). The water content was determined before cryopreservation, while the germination percentage, germination speed index (GSI), and total soluble carbohydrates were quantified after cryopreservation. The highest percentage of moisture (17.6%) was observed in 120 DAA, while the highest percentage of germination (89.6%) and GSI (17.0) were observed in 150 DAA. Optical and transmission electron microscopy analyses were performed, and no cell damage or changes at the morpho-histological and ultrastructural levels were observed after the cryopreservation process. From these results, V. reitzii seeds can be classified as orthodox seeds and the cryopreservation (+LN) is an efficient tool for an ex situ conservation of this species.

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Section: Discussionsupporting

confidence: 53%

“…& Schult.f. seeds, which it was proven that the use of cryoprotectants is not necessary for cryopreservation (Ferrari et al 2016), likewise, seeds of Dyckia brevifolia Baker and Dyckia delicata Larocca & Sobral showed respectively 92% and 79% germination after cryopreservation without cryoprotectants (de Paula et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest

Montoya-Serrano

Vesco

Pescador

2022

Hoehnea

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Influence of Nitric oxide donor nanoencapsulation on Dyckia excelsa Lema (Bromeliaceae) germination

Monteiro¹,

Paula

Shimizu

et al. 2023

Agron. Sci. Biotechnol.

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Bromeliads are used in landscaping due to the beauty of their leaves and flowers. The use of plant regulators such as nitric oxide (NO) promotes the stimulus of germination and it has been a way out to enhance production and reduce the search for plants in nature and nanoencapsulation aims to optimize its effect. The objective of this work was to evaluate the effect of using free and nanoencapsulated NO donor on the germination of D. excelsa. The treatments consisted of soaking the seeds for 5 minutes with s-nitrosoglutathione (GSNO), chitosan/sodium tripolyphosphate nanoparticles containing GSNO (NP CS/TPP-GSNO) and empty (NP CS/TPP) at doses: 15 mM and 20 mM. The control consisted of imbibition in distilled water. For each treatment, 4 replications of 50 seeds were used. The following variables were evaluated: percentage of germination (GER), first germination count (FGC), germination speed index (GSI), mean germination time (t) in addition to the length (SL) and seedling dry weight (SDW). To characterize the seeds, water content and viability were evaluated. D. excelsa seeds had 9.9% water content and 64% viability. For GER, treatments with GSNO ranged from 43 to 60%. The application of GSNO stimulated the germination process of D. excelsa and the nanoencapsulation did not cause any difference in the results compared to the free GSNO. It was concluded that the application

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In Vitro Germination and Propagation of Dyckia brevifolia, An Ornamental and Endangered Bromeliad

Bertsouklis

Panagaki

2022

Horticulturae

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Dyckia brevifolia is an endangered plant used for ornamental purposes. As no references to the in vitro propagation of the species exist, the present study aims at investigating the possibility of an efficient micropropagation protocol. Seeds collected from mother plants were germinated at high percentages (84–86%) at a range of 15–25 °C, without any pre-treatment, and demonstrated their highest germination speed index (191.51) at 25 °C. In vitro-grown seedlings were used as the starting material for micropropagation on solid, or liquid, MS medium, supplemented with a variety of concentrations of cytokinins (BA, KIN or 2IP). Shoots and leaves were used as starting explants. Liquid media supplemented with BA or 2IP at 1.0 mg L−1 led to high multiplication rate and 2.7, or 2.3, lateral shoots were regenerated while on 2IP a high percentage (77.5%) of rooting occurred at the same time. Rooted microshoots were acclimatised ex vitro at 100% and acclimatised plants were transplanted in pots where they grew with a survival rate of 100% after two months. The in vitro propagation protocol presented in this study could enhance the large-scale propagation use of D. brevifolia as an ornamental plant and, simultaneously, contribute to the ex-situ conservation of the species.

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Cryopreservation of seeds from endangered Brazilian bromeliads - Dyckia brevifolia Baker and D. delicata Larocca & Sobral

Cited by 3 publications

References 19 publications

Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest

Seeds cryopreservation of Vriesea reitzii Leme & A.F. Costa endemic bromeliad from Atlantic Rainforest

Influence of Nitric oxide donor nanoencapsulation on Dyckia excelsa Lema (Bromeliaceae) germination

In Vitro Germination and Propagation of Dyckia brevifolia, An Ornamental and Endangered Bromeliad

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