CRYOPRESERVATION OF SEEDS OF THE BRAZILIAN NATIVE SPECIES AROEIRA-DO-SERTÃO (Astronium urundeuva M. Allemão Engl.)1

Paula, Jean Carlo Baudraz de; Guariz, Hugo Roldi; Júnior, Walter Aparecido Ribeiro; Shimizu, Gabriel Danilo; Faria, Ricardo Tadeu de; Oliveira, Halley Caixeta

doi:10.1590/1983-21252022v35n419rc

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…In the germination process of A. urundeuva seeds, for so-called normal seedlings, there was a peak after 7 days of in vitro cultivation that extended to the 23rd, in which it observed a germination stabilization, totaling 240 seedlings (Figure 5). This behavior can be explained because the Aroeira diaspores have an average seedling emergence rate after 5 days of sowing (Paula et al, 2022). Over time, diaspores that are not viable, even if provided all the ideal conditions, there is no seedling formation.…”

Section: Germination In Vitromentioning

confidence: 99%

GERMINAÇÃO E BALANÇO HORMONAL in vitro DE Astronium urundeuva (M. Allemão) Engl.

Silva

Oliveira

Nogueira

et al. 2023

Braz. J. Prod. Eng.

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Aroeira-do-sertão (Astronium urundeuva) apresenta aplicabilidade para fins terapêuticos devido às suas características farmacológicas e para uso madeireiro em razão da sua resistência e com grande durabilidade. Isso acarretou a intensa exploração da espécie, levando-a a ser incluída na lista de espécies ameaçadas de extinção. Em decorrência desta situação, a micropropagação é tida como ferramenta em potencial para a propagação e conservação in vitro da espécie. Diante disso, o objetivo deste trabalho foi avaliar a germinação e o efeito de diferentes concentrações de ANA associada com TDZ e BAP na multiplicação in vitro de A. urundeuva. Segmentos nodais, obtidos a partir de plântulas germinadas in vitro, foram utilizados como explantes. Os mesmos foram inoculados no meio de cultura MS50%, suplementado com as seguintes concentrações de ANA (0,0; 0,025 e 0,050 mg.L-1) associadas com TDZ (0,0; 0,25; 0,50; 0,75 e 1,00 mg.L-1) e BAP (0,0; 0,25; 0,50; 0,75 e 1,00 mg.L-1). Transcorridos 31 dias, foi avaliado o vigor dos explantes, calejamento e número de brotações. O percentual de germinação in vitro das sementes de A. urundeuva foi de 61%, demostrando a viabilidade da técnica para iniciar a micropropagação. O maior vigor dos explantes foi obtido no meio de cultura suplementado somente com BAP, por sua vez, o TDZ induziu distúrbios fisiológicos nos explantes, como intenso calejamento e posterior necrose dos tecidos. Os resultados obtidos indicam a necessidade de maiores estudos em razão do balanço hormonal auxina/citocinas não foi favorável para a multiplicação in vitro da espécie.

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Section: Germination In Vitromentioning

confidence: 99%

GERMINAÇÃO E BALANÇO HORMONAL in vitro DE Astronium urundeuva (M. Allemão) Engl.

Silva

Oliveira

Nogueira

et al. 2023

Braz. J. Prod. Eng.

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Unlocking the Potential of Phloroglucinol and Tocopherol in Improving the Photosynthetic Efficiency, Membrane Stability, and Antioxidant Enzyme Activities of Cryopreserved In Vitro Raised Plantlets of Citrus maxima Merr. And Citrus jambhiri Lush

Sanglyne,

Kurbah,

Das

2023

Preprint

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This study examined the effects of tocopherol (Vitamin E) and phloroglucinol (1,3,5-trihydroxybenzene) on cryopreserved plantlets of Citrus jambhiri Lush and Citrus Maxima Merr., two important Citrusspecies used in citrus breeding and variety development. After undergoing cryopreservation using conventional desiccation methods, the seeds of these two species were propagated in Murashige and Skoog (MS) medium with various concentrations of tocopherol and phloroglucinol. Plantlets raised from both cryopreserved and non-cryopreserved seeds were multiplied using clonal propagation on MS medium supplemented with the specified two chemicals. The clones were subsequently assessed by a range of procedures, including the quantification of chlorophyll levels, measurement of electrolyte leakage, and evaluation of specific antioxidant enzyme activities, including superoxide dismutase, catalase, and peroxidase. Cryopreserved and non-cryopreserved C. jambhiri and C. maxima clones subjected to 2.0 µM and 15.0 µM tocopherol showed improved membrane stability compared to their control counterparts. Increased photosynthetic efficiency was attained by supplementing 0.3 µM and 4.0 µM phloroglucinol to C. jambhiri and C. maxima, respectively. Both cryopreserved and non-cryopreserved C. jambhiri clones had their maximum antioxidant enzyme activity when cultured in a medium with 2.0 µM tocopherol and 0.3 µM phloroglucinol. In C. maxima, cryopreserved and non-cryopreserved clones had higher antioxidant enzyme activity in medium with 15.0 µM tocopherol and 4.0 µM phloroglucinol. In conclusion, the use of vitamin E and phloroglucinol in cryopreservation can greatly improve the important developmental traits of cryopreserved citrus plantlets.

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Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds

Campos,

Pereira,

El Merzougui

et al. 2024

Horticulturae

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In response to the near-threatened status of Handroanthus impetiginosus, primarily due to habitat loss and illegal logging, this study examines how X-ray imaging and cryopreservation impact the seed quality and viability essential for conservation. Seeds initially had a moisture content of 12.3%, reduced to 6.5% through desiccation. X-ray imaging allowed for detailed visualization of internal structures, identifying seeds as normal, abnormal, or dead based on damage and development. Normal seeds consistently germinated and produced healthy seedlings, while those with internal damage or excessive desiccation either resulted in abnormal seedlings or did not germinate. Various cryopreservation treatments were tested, including storage at −80 °C and liquid nitrogen immersion (LN), with and without vitrification solutions (PSV2; PVS3; PSV2 + 1% phloroglucinol; PSV3 + 1% phloroglucinol). Results indicated that immersion in LN without cryoprotectants achieved the highest germination and seedling viability, whereas vitrification solutions, such as PVS2 and PVS3, negatively affected germination. This study demonstrates that X-ray imaging is an effective tool for assessing seed quality and detecting internal damage, while cryopreservation without cryoprotectants is suitable for long-term seed storage. This work highlights the benefits of combining X-ray assessment with optimized cryopreservation techniques to support the conservation of threatened species.

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CRYOPRESERVATION OF SEEDS OF THE BRAZILIAN NATIVE SPECIES AROEIRA-DO-SERTÃO (Astronium urundeuva M. Allemão Engl.)1

Cited by 4 publications

References 22 publications

GERMINAÇÃO E BALANÇO HORMONAL in vitro DE Astronium urundeuva (M. Allemão) Engl.

GERMINAÇÃO E BALANÇO HORMONAL in vitro DE Astronium urundeuva (M. Allemão) Engl.

Unlocking the Potential of Phloroglucinol and Tocopherol in Improving the Photosynthetic Efficiency, Membrane Stability, and Antioxidant Enzyme Activities of Cryopreserved In Vitro Raised Plantlets of Citrus maxima Merr. And Citrus jambhiri Lush

Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds

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