Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão, R.; Marques, C.C.; Bettencourt, E.M.; Pereira, Rúben

doi:10.5772/62121

Cited by 5 publications

(3 citation statements)

References 199 publications

(362 reference statements)

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“…Over the past decades, an increasing demand for ART application in livestock has been observed [8,46,47]. Additionally, delayed childbearing associated to advanced maternal age has now become the main factor leading women to resort to ART [5].…”

Section: Discussionmentioning

confidence: 99%

Anti-Aging Effect of Urolithin A on Bovine Oocytes In Vitro

Fonseca

Marques

Pimenta

et al. 2021

Animals

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Oxidative stress and mitochondrial dysfunction have been associated with the age-related decline of oocyte quality and strategies for their prevention are currently quested. Urolithin A (UA) is a natural metabolite with pro-apoptotic and antioxidant effects, capable of preventing the accumulation of dysfunctional mitochondria in different aged cells. UA has never been tested in bovine oocytes. Our aim was to study the effect of UA on the developmental potential of cumulus-oocyte-complexes (COCs) and granulosa cells’ (GCs) expression of important genes related to reproductive competence. Nuclear maturation progression, mitochondrial membrane potential (MMP) and developmental competence of physiologically mature (22 h) and in vitro aged oocytes (30 h of IVM) obtained from prepubertal and adult females, either supplemented with UA or not were assessed. Additionally, the amount of mRNA of several genes (NFE2L2, NQO1, and mt-DN5) and the number of mt-ND5 DNA copies were quantified in cultured GCs from prepubertal and adult females, either supplemented with UA or not. Our study confirmed the harmful effect of oocyte aging on the nuclear maturation progression, MMP, developmental competence and gene expression levels. UA treatment during in vitro maturation enhanced (p < 0.05) the maturation rate and subsequent developmental capacity of aged oocytes. A positive effect (p < 0.05) of UA on physiological maturation, MMP and embryonic development was also identified. UA also interfered on the expression profile of NFE2L2 and NQO1 genes in GCs cultures. Our findings demonstrate that UA supplementation is an effective way to prevent oocyte aging and improves the subsequent bovine embryonic development.

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Section: Discussionmentioning

confidence: 99%

Anti-Aging Effect of Urolithin A on Bovine Oocytes In Vitro

Fonseca

Marques

Pimenta

et al. 2021

Animals

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“…Experiments involving cow [36] and goat [37,38] embryos have shown that the survival of vitrified embryos can be related to several factors, such as the use of different cryoprotectants, exposure time or concentration of vitrification solutions or different stages of embryonic development. Researchers cite several protocols and technical options to improve embryo survival after cryopreservation in goats [39] and sheep [40,41].…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation and Transfer of Sheep Embryos Recovered at Different Stages of Development and Cryopreserved Using Different Techniques

Selionova

Aibazov

Zharkova

2023

Animals

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This article presents data from experiments to determine the cryoresistance of Charollais sheep embryos, depending on the stage of embryo development and the method of freezing, as well as the results of embryo transfer. The study design consisted of a study on the cryopreservation of ewe embryos at different developmental stages (early, 2–8 blastomeric and late, at the morula/blastocyst stage), two cryopreservation protocols (slow freezing and ultra-fast vitrification), and embryo transfer of cryo- and fresh embryos. Embryos from Charollais sheep donors (n = 12) were recovered after induction of superovulation. The embryos were recovered surgically (laparotomy) on days 2 and 6 after insemination. Before there was transfer to recipients, part of embryos was cryopreserved using standard slow freezing and ultra-fast vitrification methods. The average ovarian response was 7.54 ovulations per donor, and 5.83 embryos per donor were collected. No effect of the cryopreservation method and embryo development stage on the preservation of the morphological structure of embryos was found. There were no significant differences in the survival rate of cryoembryos at different development stages, frozen using different techniques, and after transfer to recipients. Differences in cryoresistance between embryonic developmental stages in favor of the morula/blastocyst stage were found (survival after thawing 86.4% vs. 75.0% in early embryos). At different stages of development, the survival rate of fresh embryos (45.8%) compared to cryopreserved ones (30.2%) was significantly higher (p < 0.05), while among fresh ones, the best survival rate (50.0%) was observed after the transfer of morules and blastocysts.

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“…For instance, in cattle, lipolytic agents or chemical delipidators have been successfully applied, increasing the cryotolerance of vitrified embryos (phenazine ethosulfate [212], trans-10 cis-12 conjugated linoleic acid (CLA) [22,208], and forskolin [213]). These chemicals that regulate metabolism were used to reduce embryo lipid content, inducing smaller lipid droplets and fat indexes, thus improving embryonic cryosurvival [22,208,214]. The addition of CLA during culture of sheep IVP embryos also increased their cryotolerance [8].…”

Section: Methods Of Improving the Cryopreservation Of Sheep Embryosmentioning

confidence: 99%

Insights from Animal Reproduction

Payan-Carreira¹

2016

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The litter size is an important trait in prolific species such as rabbits and pigs. However, selection on litter size has had limited success in these species because of its low heritabil-ity and sex-limited expression. The litter size is a complex physiological trait in prolific species, affected by several components that are expressed sequentially, for example, ov-ulation, fertilization, embryo development, and fetal survival. The selection for ovulation rate or/and prenatal survival has been proposed to improve litter size indirectly. However , these alternative methods have not reached the expected response rate. Implantation is also a critical point in successful gestation, one-third to one-half of prenatal mortality occurring during peri-implantation. The uterus must provide an adequate microenviro-ment for the growth and development of embryo and for receptivity to implantation. There are multitudes of cellular events involved in crosstalk between embryo and maternal uterus during peri-implantation. A better understanding of molecular mechanisms affecting the implantation process could help to propose new strategies for litter size improvement in prolific species.

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Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Cited by 5 publications

References 199 publications

Anti-Aging Effect of Urolithin A on Bovine Oocytes In Vitro

Anti-Aging Effect of Urolithin A on Bovine Oocytes In Vitro

Cryopreservation and Transfer of Sheep Embryos Recovered at Different Stages of Development and Cryopreserved Using Different Techniques

Insights from Animal Reproduction

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