Cryopreservation of Sperm as a Means to Store Salmonid Germ Plasm and to Transfer Genes from Wild Fish to Hatchery Populations

Cloud, J. G.; Miller, William H.; Levanduski, Mike

doi:10.1577/1548-8640(1990)052<0051:cosaam>2.3.co;2

Cited by 50 publications

(32 citation statements)

References 5 publications

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“…In vitro fertilizations were performed by using semen samples from males of both treatment groups (control and EE2 exposed) as described by Cloud et al (77). Nine days after fertilization, developing embryos were prepared for nucleolar organizer region (NOR) and chromosome staining as described in Thorgaard and Disney (78).…”

Section: Fertilization and Embryonic Tissue Preparationsmentioning

confidence: 99%

Aneuploid sperm formation in rainbow trout exposed to the environmental estrogen 17α-ethynylestradiol

Brown

Schultz

Cloud

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Environmental contaminants that mimic native estrogens (i.e., environmental estrogens) are known to significantly impact a wide range of vertebrate species and have been implicated as a source for increasing human male reproductive deficiencies and diseases. Despite the widespread occurrence of environmental estrogens and recognized detrimental effects on male vertebrate reproduction, no specific mechanism has been determined indicating how reduced fertility and/or fecundity is achieved. Previous studies show that male rainbow trout, Oncorhynchus mykiss, exposed to the environmental estrogen 17␣-ethynylestradiol (EE2) before gamete formation and fertilization produce progeny with significantly reduced embryonic survival. To determine whether this observed decrease results from sperm chromosome alterations during spermatogenesis, male rainbow trout were exposed to 10 ng of EE2/l for 50 days. After exposure, semen was collected and sperm aneuploidy levels analyzed with two chromosome markers by fluorescent in situ hybridization. In vitro fertilizations were also conducted by using control and exposed sperm crossed to eggs from an unexposed female for offspring analysis. Evaluations for nucleolar organizer region number and karyotype were performed on developing embryos to determine whether sperm aneuploidy translated into embryonic aneuploidy. Results conclusively show increased aneuploid sperm formation due to EE2 exposure. Additionally, embryonic cells from propagated progeny of individuals possessing elevated sperm aneuploidy display high levels of embryonic aneuploidy. This study concludes that EE2 exposure in sexually developing male rainbow trout increases levels of aneuploid sperm, providing a mechanism for decreased embryonic survival and ultimately diminished reproductive success in EE2 exposed males.aneuploidy ͉ EE2 ͉ xenoestrogens

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Section: Fertilization and Embryonic Tissue Preparationsmentioning

confidence: 99%

Aneuploid sperm formation in rainbow trout exposed to the environmental estrogen 17α-ethynylestradiol

Brown

Schultz

Cloud

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

“…Cryopreservation of milt from male donors has been used in the captive rearing program since 1997 and follows techniques described by Cloud et al (1990) and Wheeler and Thorgaard (1991). Milt is cryopreserved and stored at three locations (Eagle Fish Hatchery, University of Idaho, and Washington State University) to spread the risk associated with freezing technique error and storage system failure.…”

Section: Cryopreservationmentioning

confidence: 99%

Captive Rearing Initiative for Salmon River Chinook Salmon, 1999 Progress Report.

Hassemer¹

2001

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“…Cryopreservation of milt from male donors has been used in the captive rearing program since 1997 and follows standard techniques (Cloud et al 1990;Wheeler and Thorgaard 1991). Cryopreserved milt is stored at the Eagle Fish Hatchery.…”

Section: Cryopreservationmentioning

confidence: 99%

Captive Rearing Program for Salmon River Chinook Salmon, 2000 Project Progress Report.

Venditti¹

2002

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Cryopreservation of Sperm as a Means to Store Salmonid Germ Plasm and to Transfer Genes from Wild Fish to Hatchery Populations

Cited by 50 publications

References 5 publications

Aneuploid sperm formation in rainbow trout exposed to the environmental estrogen 17α-ethynylestradiol

Aneuploid sperm formation in rainbow trout exposed to the environmental estrogen 17α-ethynylestradiol

Captive Rearing Initiative for Salmon River Chinook Salmon, 1999 Progress Report.

Captive Rearing Program for Salmon River Chinook Salmon, 2000 Project Progress Report.

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