Cryopreservation of spotted halibut (Verasper variegatus) sperm

Tian, Yongsheng; Chen, S.L.; Ji, Xiang‐Shan; Zhai, Jieming; Sun, Liang-zhen; Chen, C.; Su, P.Z.

doi:10.1016/j.aquaculture.2008.07.047

Cited by 30 publications

(27 citation statements)

References 14 publications

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“…Our results regarding survival rate and motility of Taiwan shoveljaw carp spermatozoa after cryopreservation demonstrated that DMSO was the best cryoprotectant in the freezing experiments. These results agree with several previous findings where DMSO has been used successfully with spermatozoa of many fish species (Muchlisin 2005;Horváth et al 2003;Melo and Godinho 2006;Ji et al 2008;Tian et al 2008). Although methanol has been used successfully as a cryoprotectant for the cryopreservation of spermatozoa of channel catfish (Christensen and Tiersch 2005), bitterling (Ohta et al 2001) and bagrid catfish (Muchlisin and Muhammadar 2002), in our study cryopreservation with methanol appeared to be less efficient than DMSO, DMA and glycerol in terms of survival rate and motility.…”

Section: Discussionsupporting

confidence: 77%

“…• C min −1 ) result in better survival of spotted halibut, neotropical genera and Xiphophorus spermatozoa in cryopreservation (Melo and Godinho 2006;Tian et al 2008;Tiersch and Yang 2009).…”

Section: Discussionmentioning

confidence: 99%

“…However, increasing the volume of frozen-thawed spermatozoa to give a ratio of 1 : 2 × 10 6 improved the fertilisation and hatching rate, which were then not significantly different from results with fresh spermatozoa. Frozen-thawed spermatozoa are more fragile than fresh spermatozoa because they have suffered the effects of freezing (Tian et al 2008). Therefore, a higher density of frozen-thawed spermatozoa would be needed than fresh spermatozoa to compensate for this and allow successful fertilisation results (Chao and Liao 2001).…”

Section: Discussionmentioning

confidence: 99%

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Effects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus) spermatozoa

Tsai

Spikings

Hwang

et al. 2009

Aquat. Living Resour.

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-Over the past decades, pollution, overfishing, and habitat degradation have driven the population size of Taiwan shoveljaw carp down markedly in Taiwan. Cryopreservation is a useful tool which could be used to maintain genetic resources to protect and preserve this endemic species. Four cryoprotectants [dimethyl sulphoxide (DMSO), dimethylacetamide (DMA), glycerol and methanol] and six freezing rates (0.5, 1, 2, 4, 8, 16• C min −1 ) were tested in order to develop an optimal controlled slow-freezing protocol for Taiwan shoveljaw carp spermatozoa. Samples were subsequently examined under the scanning electron microscope to reveal whether cryopreservation had affected their ultrastructural morphology. The highest survival rate ( 50.1±2.0%) was observed with a freezing rate of 8• C min −1 in 1M DMSO, using SYBR-14 + PI staining. Fertility and hatching rate results using frozen-thawed spermatozoa (90.2± 2.2% and 22.3 ± 2.5%, respectively) were not significantly different from results with fresh spermatozoa. After cryopreservation, 21.0 ± 1.6% of frozen-thawed spermatozoa had mid-piece swelling and rupture of the head. Cryopreservation might, therefore, slightly affect Taiwan shoveljaw carp spermatozoa in terms of morphological change. However, these alterations could be compensated by using large enough numbers of normally functioning frozen-thawed spermatozoa to achieve a standard equal to fresh spermatozoa. This is the first report of successful cryopreservation of Taiwan shoveljaw carp spermatozoa using a controlled slow-cooling method.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus) spermatozoa

Tsai

Spikings

Hwang

et al. 2009

Aquat. Living Resour.

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“…Spotted halibut, Verasper variegatus (Temminck et Schlegel), is a marine flatfish and mostly distributed in coastal areas of China, Japan, and Korea (Wada et al 2004;Tian et al 2008). Nowadays, it has become one of the main aquaculture species in northern China.…”

Section: Introductionmentioning

confidence: 99%

Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus)

Chen

Yang

et al. 2010

Aquacult Int

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In the present study, methodology of gynogenetic induction in spotted halibut were developed and optimized; the sex ratio of putative meiotic gynogenetic diploids was determined using AFLP-based molecular sexing technique; the homozygosity of gynogenetic population was assessed as opposed to cultivated population. The results showed that high percentage of meiotic gynogenetic diploids were generated when the eggs fertilized with irradiated heterologous sea perch frozen sperm (30-50 mJ cm -2 ) were cold shocked in sea water of -1°C for 40-75 min at 5 min after fertilization. About 15,200 diploid gynogenetic larvae were achieved and they exhibited normal morphology similar to diploid control. The gynogenetic diploids were 100% female, which first confirmed the female homogamete (XX/XY) sex determination in spotted halibut. The genetic analysis showed that the average H O was, respectively, 0.404 and 0.724 in gynogenetic population and cultivated population, indicating an increase of homozygosity in gynogenetic population.

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“…Estos resultados concuerdan con aquellos obtenidos en diversos peces planos como lenguado de invierno Pseudopleuronectes americanus (Rideout et al, 2003), rodaballo Scophthalmus maximus ; lenguado de Argentina P. orbignyanus (Lanes et al, 2008) y róbalo manchado Verasper variegatus (Tian et al, 2008). Similares resultados fueron obtenidos por nuestro grupo de trabajo al criopreservar espermatozoides de anchoveta Engraulis ringens (Catcoparco et al, 2010).…”

Section: Introductionunclassified

Criopreservacion de espermatozoides del lenguado Paralichthys adspersus

Catcoparco

Silva

Dupré

2012

lajar

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RESUMEN. Para optimizar las técnicas de reproducción en cautiverio de lenguado Paralichthys adspersus; se elaboró una metodología para la criopreservación de sus espermatozoides. Para ello, se evaluó el efecto del dimetil sulfóxido (DMSO) como agente crioprotector en tres concentraciones diferentes (1,0; 1,5 y 2,0 M) sobre la motilidad espermática y su posterior congelación con cinco diferentes tasas: -7,5; -10; -12,5; -20 y -30°C min -1 , utilizando un congelador automático programable. Los mayores porcentajes de motilidad espermática post congelamiento-descongelamiento (40,5 ± 13%) fueron obtenidos al utilizar una tasa de -10°C min -1 y DMSO sin encontrar diferencias significativas entre las tres diferentes concentraciones de DMSO (P < 0,05). Posteriormente, se evaluó el efecto de un crioaditivo no permeable (vitelo de huevo de gallina o VHG), para determinar si era posible incrementar los porcentajes de motilidad espermática. Se utilizó una solución crioprotectora compuesta por DMSO, en cada una de las tres concentraciones diferentes, adicionando VHG al 10% v/v. Los mayores porcentajes de motilidad espermática (71,71 ± 13%) también se obtuvieron a la tasa de congelamiento de -10°C min -1 , sin encontrar diferencias significativas entre las concentraciones de la solución crioprotectora en la cual fue incubada la muestra de espermatozoides (P < 0,05). Al comparar los porcentajes de motilidad espermática post-descongelación utilizando el crioprotectante DMSO, se obtuvieron diferencias significativas cuando se agregó VHG. Palabras clave: crioaditivos, crioprotectantes, motilidad espermática, tasa de congelamiento, lenguado, Chile. Cryopreservation of flounder Paralichthys adspersus spermatozoaABSTRACT. To optimize the techniques of captive breeding of flounder Paralichthys adspersus, a methodology was developed for the cryopreservation of spermatozoa of this species. The effect on sperm motility post-thawing, using three different concentrations (1.0; 1.5 and 2.0 M) of DMSO as cryoprotective agent and five different freezing rates -7.5; -10; -12.5; -20 and -30°C min -1 , with an automatic programmable freezer was evaluated. The highest percentages of post-thawing sperm motility were obtained by freezing sperm at -10°C min -1 , no significant differences (P < 0.05) were founded between the three different concentrations of DMSO used. Subsequently, we evaluated the effect of a non-permeable cryo-additive (chicken egg yolk, VHG), in order to obtain an increasing of the percentage of sperm motility. We used a cryoprotectant solution including DMSO at three different concentrations adding 10% VHG v/v. The highest percentages of sperm motility (71.71 ± 13%) were obtained at the freezing rate of -10°C min -1 without significant differences between the three concentrations of the cryoprotectant solution in which the sample was incubated sperm (P < 0.05). A high significant difference between the sperm motility percentages postthawing using DMSO and DMSO with VHG, was observed.

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Cryopreservation of spotted halibut (Verasper variegatus) sperm

Cited by 30 publications

References 14 publications

Effects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus) spermatozoa

Effects of the slow cooling during cryopreservation on the survival and morphology of Taiwan shoveljaw carp (Varicorhinus barbatulus) spermatozoa

Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus)

Criopreservacion de espermatozoides del lenguado Paralichthys adspersus

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