Cryopreservation of stallion sperm using different amides

Medeiros, A. S. L.; Gomes, Gustavo Mendes; Carmo, M. T.; Papa, Frederico Ozanam; Alvarenga, Marco Antônio

doi:10.1016/s0093-691x(02)00898-1

Cited by 55 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, using a combination of DMSO and DMF at 4% would minimise the possibilities of harmful interaction between DMSO and the egg yolk. Contrary to our results, previous studies in stallions showed that supplementation of INRA-82 extender with DMF alone improved the quality of cryopreserved semen (Medeiros et al, 2002;Squires et al, 2004;Alvarenga et al, 2005;Mesa and Henao, 2012;Fadl, 2016). The discrepancies between the results may be due to the species differences.…”

Section: Discussioncontrasting

confidence: 99%

Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants

Fadl

Ghallab

Abou-Ahmed³

2019

World Rabbit Sci.

View full text Add to dashboard Cite

<p>The present study aimed to evaluate the effect of supplementation of INRA-82 semen extender with different cryoprotectants (dimethyl sulphoxide; DMSO vs. dimethyl formamide; DMF) on the quality of white New Zealand rabbit buck spermatozoa. We also investigated the possible association between the synergistic action of DMSO and DMF and their relation with INRA-82 extender composition. Semen was collected and pooled from 8 adult rabbit bucks. Pooled semen samples were diluted 1:1 with INRA-82 extender supplemented with DMSO 8%, DMF 8% or a combination of DMSO 4% and DMF 4%. The diluted semen samples were cryopreserved in 0.25 plastic straws. After thawing, progressive motility, sperm viability, sperm abnormalities, membrane integrity, acrosome status, viability index and DNA integrity were evaluated. The results showed that dilution of rabbit buck semen in INRA-82 supplemented with DMSO and DMF (4% each) before freezing significantly (<em>P</em><0.05) improved sperm motility (42.00%), percentage of live spermatozoa (45.30%), proportions of spermatozoa with intact acrosome (59.75%) and percentage of spermatozoa with non-fragmented DNA (86.04%), compared to those diluted in INRA-82 supplemented either with DMSO 8% (+9, +10, +5 and +7 percentage points, respectively) or with DMF 8% alone (+18 +18, +12 and +9 percentage points, respectively). In conclusion, dilution of rabbit buck semen before freezing with INRA-82 extender supplemented with a combination of DMSO 4% and DMF 4% improved quality of frozen-thawed New Zealand White rabbit spermatozoa. Furthermore, our results also suggest that supplementation of INRA-82 with DMSO or with DMF alone at higher concentrations deteriorates the sperm quality.</p>

show abstract

Section: Discussioncontrasting

confidence: 99%

Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants

Fadl

Ghallab

Abou-Ahmed³

2019

World Rabbit Sci.

View full text Add to dashboard Cite

show abstract

“…The action of glycerol in reducing the freeze-thaw damage to cells is similar to that of both nonpermeating (Breddermann & Foote, 1969) and permeating (Medeiros, Gomes, Carmo, Papa, & Alvarenga, 2002) CPAs. On the other hand, glycerol at high concentrations can lead to programmed cell death (Wundrich, Paasch, Leicht, & Glande, 2006).…”

Section: Discussionmentioning

confidence: 77%

Comparison of different freezing techniques, extenders, and cryoprotectants on quality and fertility of cryopreserved Salmo trutta f. fario sperm

Bozkurt,

Yavaş

2023

Acta Sci Technol

View full text Add to dashboard Cite

There is still a lack of standardized methodology in the cryopreservation of Salmo trutta f. fario sperm. Thus, the present study was designed to compare current freezing protocols to improve and as well as to examine the post-thaw quality and fertilizing ability of cryopreserved sperm in Salmo trutta f. fario. Sperm samples were diluted at a 1:10 ratio in one of three extenders (Alsever’s solution, glucose-based, and ionic-based) containing four types of cryoprotectant (DMSO, DMA, MeOH, glycerol) at 10% concentration and frozen in 0.1 mL pellets on surface of the dry ice (solid carbon dioxide, 79°C) or in 0.25 mL straws 2 cm above of the liquid nitrogen (LN2) surface at a rate of ~30°C for 10 min.1 before storage in a mL cryotank (-196°C). The frozen sperm cells in straws and pellets were thawed in a water bath at 25°C for 30 s and at 20°C for 6 s respectively. Fertilization was carried out using a ratio of 5x105 sperm/egg in both freezing methods. The glucose-based solution including glycerol produced the highest post-thaw progressive motility (62.5 ± 1.24%), motility duration (57.2 ± 0.46 s), and viability (56.4 ± 1.57%) (p < 005) in the straw method. In breeding trials, similarly, sperm frozen-thawed with the glucose-based solution including glycerol produced the highest fertilization (54.2 ± 0.36%) and hatching (30.6 ± 0.28%) in the straw method. Fresh sperm used as control produced 82.6 ± 0.45% and 78.4 ± 1.27% fertilization and hatching respectively. It was concluded that sperm frozen in straws produced higher post-thaw sperm motility and fertility of eggs than those frozen in pellets. Also, the results suggest using of glycerol-supplemented glucose solution because of producing better results in both freezing techniques.

show abstract

“…Semen cryopreservation is a main technique used in genetic improvement programs, preservation of valuable transgenic lines and dissemination of valuable genetic material in livestock. During the freezing process, sperms are subjected to cold shock that induces biochemical and functional damages in sperms that reduce their post‐thawing quality, including motility, abnormality, plasma membrane, acrosome and DNA integrities and subsequently fertility (Bailey et al, 2000; Bernardini et al, 2011; Celeghini et al, 2007; Medeiros et al, 2002; Salamon & Maxwell, 2000; Tekin, 2006; Watson, 2000). Briefly, the cold shock causes a significant reorganization of the intramembrane components, which disrupts cell signalling, causes lipid–lipid agglutinations and forms particle‐free zones that reduce the integrity and selective permeability of the plasma membrane, allowing unrestricted transportation of ions across it (Watson, 2000).…”

Section: Introductionmentioning

confidence: 99%

Correlation between fatty acids levels in chicken, duck, goose, pigeon, quail and turkey egg yolks and post‐thawed quality of ram semen

Swelum

Ba-Awadh

Olarinre

et al. 2023

Reprod Domestic Animals

View full text Add to dashboard Cite

The comparison between adding egg yolks (EY) of chicken, duck, goose, pigeon, Japanese quail or turkey to the Tris glycerol extender on the quality of ram semen before freezing and post‐thawing was evaluated. The correlation between fatty acids levels in egg yolks of different avian species and the post‐thawed quality of ram semen was studied. The pooled ejaculates collected from five rams were extended with Tris EY glycerol extenders containing the EY of chicken, duck, goose, pigeon, quail or turkey and cryopreserved at −196°C. The straws were evaluated before freezing and post‐thawing for sperm motility using a sperm cell analyser, vitality using a FluoVit kit and abnormality using a SpermBlue stain besides plasma‐membrane and DNA integrities using a hypo‐osmotic swelling test and a Halomax kit, respectively. The moisture, ash, protein and fatty acid (FA) contents of EY of chicken, duck, goose, pigeon, quail and turkey were analysed using a gas chromatograph. The chicken and quail EY extenders significantly improved the total progressive motility (32.05 ± 1.41 and 31.68 ± 1.43, respectively), vitality, plasma membrane and DNA integrities and abnormalities of post‐thawing ram semen in comparison with other EY extenders. Pigeon EY had the lowest saturated fatty acids (SFAs) in comparison with the other types of EYs. The chicken and turkey EYs had the lowest percentage of (monounsaturated fatty acids) MUFAs in comparison with the other types of EYs. The highest percentage of polyunsaturated fatty acids (PUFAs) was observed in the turkey, pigeon and chicken EYs which were considered double or triple their percentage in duck and goose EYs, respectively. Significant positive correlations existed between SFAs levels and total motility, vitality, plasma membrane functionality and DNA integrity (0.77, 0.80, 0.67, 0.52, respectively). Significant negative correlations existed between gondoic EY levels and total motility, vitality, plasma membrane functionality and DNA integrity. In conclusion, the EYs of duck, goose, pigeon or turkey cannot substitute the chicken EY in ram semen extenders as they gave lower post‐thawing quality. The quail EY can be used as a good replacer for chicken EY in the extender used for cryopreservation of ram semen. The EY composition of FAs can significantly affect the quality of ram semen post‐thawing.

show abstract

Cryopreservation of stallion sperm using different amides

Cited by 55 publications

References 0 publications

Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants

Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants

Comparison of different freezing techniques, extenders, and cryoprotectants on quality and fertility of cryopreserved Salmo trutta f. fario sperm

Correlation between fatty acids levels in chicken, duck, goose, pigeon, quail and turkey egg yolks and post‐thawed quality of ram semen

Contact Info

Product

Resources

About