Cryopreservation of Testicular Tissue from Adult Red-Rumped Agoutis (Dasyprocta leporina Linnaeus, 1758)

Pereira, Ana G.; Bezerra, Luana Grasiele Pereira; Moreira, Samara Sandy Jerônimo; Pereira, Alexsandra Fernandes; Oliveira, Moacir Franco de; Comizzoli, Pierre; Silva, Alexandre Rodrigues

doi:10.3390/ani12060738

Cited by 6 publications

(7 citation statements)

References 36 publications

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“…Furthermore, two vitrification methods were also compared in the latter species, obtaining better results with conventional vitrification than with solid surface vitrification ( 10 ). In red-rumple agouti, vitrification was superior than slow freezing ( 11 ). Contrary to collared peccary, solid surface vitrification in red-rumple agouti testes provided greater results than conventional vitrification ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

“…In red-rumple agouti, vitrification was superior than slow freezing ( 11 ). Contrary to collared peccary, solid surface vitrification in red-rumple agouti testes provided greater results than conventional vitrification ( 11 ). In gray wolf, slow freezing demonstrated a superior ability to protect testes than vitrification ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

“…The uncontrolled slow freezing requires a small passive cooling device to achieve a cooling rate of 1°C/min, which is less expensive than a programmable freezer (controlled slow freezing), easier and can be used outside the lab ( 4 ). For testicular tissue vitrification, the methods or protocols most employed are conventional vitrification, solid surface vitrification, and needle immersed vitrification ( 9 – 11 ). One of the advantages of slow freezing is the lower concentration of cryoprotectants in comparison to vitrification either in controlled or uncontrolled method, which considerably decreases the cytotoxic effects of these agents ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown variable results when different cryopreservation techniques were compared. In fact, the differences are also evident when different protocols or methods are used within the same technique and compared ( 10 , 11 ). In boars and cats, slow freezing and vitrification showed similar results in terms of testicular cell survival ( 2 , 13 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

“…In ram, slow freezing better preserved testicular morphology than vitrification ( 20 ). Among wild species, comparative studies have been conducted in wild boar ( 17 ), red-rumped agouti ( 11 ), collared peccary ( 10 ), gray wolf ( 9 ), jungle cat, lion, leopard, rusa deer, fea's muntjac, and sumatran serow ( 21 ) but there are many wildlife species in which comparison of cryopreservation methods are still required to better understand the species-specific differences that lead to structural and functional germ cell damage. In wild boar and collared peccary both techniques are equally effective ( 10 , 17 ).…”

Section: Introductionmentioning

confidence: 99%

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DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification

Peris-Frau

Benito-Blanco²,

Martínez‐Nevado³

et al. 2023

Front. Vet. Sci.

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Introduction and objectiveCryopreservation of testicular tissues offers new possibilities to protect endangered species, genetically valuable individuals or even the fertility potential of prepubertal individuals who have died unexpectedly. However, the use of this technique still remains a challenge. In this study, slow freezing and vitrification of testicular tissue was investigated to find out which cryopreservation method could better preserve the viability and DNA integrity of testicular germ cells in diverse wild species.MethodsTestes were obtained post-mortem from 18 artiodactyls (wild boar, roe deer, dwarf goat, mhor gazelle, European mouflon, African forest buffalo, Malayan tapir, dorcas gazelle, Iberian ibex, gnu, red river hog), 5 primates (colobus monkey, capuchin monkey, mandrill), 8 carnivores (gray wolf, Persian leopard, binturong, European mink, American black bear, suricata), and 2 rodents (Patagonian mara). The testicles belonged to adult individuals and were cut into small pieces and cryopreserved by needle immersed vitrification or uncontrolled slow freezing using a passive cooling device. After warming or thawing, testicular tissues were enzymatically digested and two germ cell types were differentiated based on their morphology: rounded cells (spermatogonia, spermatocytes, and early spermatids) and elongated cells (elongated spermatids and spermatozoa). Cell viability was assessed by SYBR-14/propidium iodide while DNA fragmentation by TUNEL assay with fluorescence microscope.Results and discussionOur preliminary results revealed that our uncontrolled slow freezing method better preserved the viability and DNA integrity of elongated cells than vitrification. Such trend was observed in all species, being significant in artiodactyls, carnivores, and primates. Similarly, the viability and DNA integrity of rounded cells was also better maintained in primates by uncontrolled slow freezing, while in carnivores, vitrification by needle immersion showed better results in this type of cells. In artiodactyls and rodents both techniques preserved the viability of rounded cells in a similar manner, although the DNA integrity of these cells was greater after needle immersed vitrification in artiodactyls.ConclusionsIn conclusion, the effectiveness of each cryopreservation method is affected by the phylogenetic diversity between species and cell type.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification

Peris-Frau

Benito-Blanco²,

Martínez‐Nevado³

et al. 2023

Front. Vet. Sci.

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Liposome-based Freezing Medium Improves the Outcome of Mouse Prepubertal Testicular Tissue Cryopreservation

Dcunha,

Mutalik,

Reji

et al. 2024

Reprod. Sci.

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Cryopreservation of testicular tissue holds an important role in the field of fertility preservation, particularly for prepubertal boys diagnosed with cancer. However, prepubertal testicular tissue cryopreservation is still considered to be in the experimental stage necessitating the refinement of cryopreservation protocol. Considering the fact that loss of membrane lipids is the primary cause of freeze–thaw-induced loss of testicular cell functions, in this study, we explored the beneficial properties of exogenous supplementation of membrane lipids in the form of liposomes in enhancing the cryosurvival of prepubertal testicular tissue. The freezing medium supplemented with liposomes (prepared from soy lecithin, phosphatidylethanolamine, phosphatidylserine, and cholesterol) was used for the experiments. Prepubertal testicular tissues from Swiss albino mice were cryopreserved in a liposome-containing freezing medium (LFM) composed of 0.25 mg/mL liposomes, 5% DMSO, and 30% FCS in the DMEM/F12 medium using a slow freezing protocol. The tissues were thawed and assessed for various testicular cell functions. Freezing in LFM mitigated the loss of viability, decreased malondialdehyde level (p < 0.05), and reduced apoptosis (p < 0.05) in the testicular cells compared to the testicular tissue cryopreserved in the control freezing medium (CFM). Further, DMSO (5%) appears to be the ideal penetrating cryoprotectant for prepubertal testicular tissue cryopreservation with liposome-based freezing medium. Similar enhancement in cryosurvival of cells was observed in adult human testicular tissue frozen with LFM. These findings highlight the translational value of liposome-based freezing medium in the cryopreservation of testicular tissue of prepubertal boys undergoing chemotherapy.

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Influence of cryopreservation techniques and low concentrations of permeating cryoprotectants on the conservation of ear cartilage and skin derived from six-banded armadillos (Euphractus sexcinctus Linnaeus, 1758)

Fernandes,

Praxedes,

Viana

et al. 2023

Cryobiology

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Cryopreservation of Testicular Tissue from Adult Red-Rumped Agoutis (Dasyprocta leporina Linnaeus, 1758)

Cited by 6 publications

References 36 publications

DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification

DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification

Liposome-based Freezing Medium Improves the Outcome of Mouse Prepubertal Testicular Tissue Cryopreservation

Influence of cryopreservation techniques and low concentrations of permeating cryoprotectants on the conservation of ear cartilage and skin derived from six-banded armadillos (Euphractus sexcinctus Linnaeus, 1758)

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