Cryopreservation of the spermatozoa ofCrassostrea tulipa and three other oysters

Yankson, Kobina; Moyse, John

doi:10.1016/0044-8486(91)90268-c

Cited by 49 publications

(26 citation statements)

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“…In a similar way to observations reported on Crassostrea tulipa (Yankson and Moyse 1991), the survival and size of blacklip pearl oyster larvae during larval rearing were not significantly different when oocytes were inseminated with fresh or thawed spermatozoa. This suggests the absence of genome alterations that might have affected embryonic development.…”

Section: Discussionsupporting

confidence: 68%

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster,Pinctada margaritifera

Hui

Vonau

Moriceau

et al. 2011

Aquat. Living Resour.

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-Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry. Spermatozoa were cryopreserved with cryoprotectant agent (CPA) 0.7 M trehalose in 0.8 M Me 2 SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23% lower than that of fresh sperm. When using thawed sperm, therefore, a higher sperm/egg ratio of 100 000:1 was required to reach 80% oocyte fertilization, compared with 100:1 for fresh sperm. Nevertheless, this first demonstration of cryopreserved sperm fertility in black pearl oyster confirms the hatchery applicability of the cryopreservation technique defined here. Monitoring for larval viability during the first 23 days of life revealed no significant differences between the progeny produced with cryopreserved sperm and that produced using fresh sperm.

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Section: Discussionsupporting

confidence: 68%

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster,Pinctada margaritifera

Hui

Vonau

Moriceau

et al. 2011

Aquat. Living Resour.

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“…Held 7 cm over LN2. Thawing at room temperature 30% Motility, 40% Survival, 57% Normal shape Yankson and Moyse (1991) Me2SO 10-20% + Gly 0.6% (v/v). Cooling rate 4.7 °C min -1 .…”

Section: Acknowledgmentsmentioning

confidence: 99%

Exploring the evolution of marine invertebrate cryopreservation – Landmarks, state of the art and future lines of research

Paredes

2015

Cryobiology

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“…Various types of CPAs have been used for preservation of the biological materials to protect from freezing damage. Dimethyl sulphoxide is widely used in gamete cryopreservation of oyster (Hughes 1973; Yankson & Moyse 1991) and marine shrimp (Anchordoguy et al . 1988), while Gly has been used with horseshoe crab (Behlmer & Brown 1984) and mud crab (Bhavanishankar & Subramoniam 1997).…”

Section: Discussionmentioning

confidence: 99%

“…EG is a low molecular weight CPA; thus, it penetrates readily into the cells. It has been used successfully in cryopreservation of the gametes of oyster (Yankson & Moyse 1991) and sea urchin (Asahina & Takahashi 1978), and of embryos and larvae of marine shrimp (Newton & Subramoniam 1996; Gwo & Lin 1998). There was no significant difference in the preservation efficiency of the 2 types of CPAs employed in the present study for the short‐term storage.…”

Section: Discussionmentioning

confidence: 99%

“…Cryopreservation of gametes in aquatic species is extensively carried out in fish aquaculture (Richardson, Wilson, Crim & Yao 1999; Viveiros, So & Komen 2000). Cryopreservation of invertebrate gametes is mainly limited to echinoderms and molluscs, such as sea urchin, Strongylocentrotus intermedius , (Ohyama & Asahina 1972; Asahina & Takahashi 1978), oyster, Crassostrea tulipa , (Hughes 1973; Yankson & Moyse 1991) and small abalone, Haliotis diversicolor , (Gwo, Chen & Cheng 2002). Owing to their economic importance, cryopreservation of spermatophores of decapod crustaceans has been attempted.…”

Section: Introductionmentioning

confidence: 99%

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Long-term cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man)

2004

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Long‐term cryopreservation of the giant freshwater prawn, Macrobrachium rosenbergii, spermatophores using glycerol (Gly) and ethylene glycol (EG) as cryoprotective agents (CPAs) was studied. The tolerance of sperm to cryopreservation was evaluated on the basis of sperm survival and fertilizing ability. The survival of the sperm was determined by trypan blue staining, while the fertilizing ability was assessed from artificial insemination of the cryopreserved spermatophores. The rates of embryo survival on day 5 after spawning and of spermatophores capable of producing embryos survived to hatching were determined. Storage of spermatophores at −20°C without CPA for a short period of up of 1–5 days decreased the sperm survival significantly and did not preserve fertilizing ability. Preservation at −20°C in the presence of 10% or 20% Gly or of 10% or 20% EG offered a simple and efficient short‐term storage up to 10 days. For a long‐term storage, cryopreservation in the presence of 20% EG at −196°C was more efficient than at −20°C. High sperm survival rates and high fertilizing ability were recorded from those cryopreserved at −196°C for up to 150 days. High sperm survival rates with moderate levels of fertilizing ability were obtained from those cryopreserved at −20°C for not more than 30 days. The results indicate that preservation at −196°C with 20% EG is a suitable procedure for long‐term storage of the giant freshwater prawn spermatophores.

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Cryopreservation of the spermatozoa ofCrassostrea tulipa and three other oysters

Cited by 49 publications

References 10 publications

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster,Pinctada margaritifera

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster,Pinctada margaritifera

Exploring the evolution of marine invertebrate cryopreservation – Landmarks, state of the art and future lines of research

Long-term cryopreservation of spermatophore of the giant freshwater prawn, Macrobrachium rosenbergii (de Man)

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