Cryopreservation of Tissue-Engineered Scaffold-Based Constructs: from Concept to Reality

Arutyunyan, I. V.; Elchaninov, Andrey; Сухих, Г. Т.; Fatkhudinov, Timur

doi:10.1007/s12015-021-10299-4

Cited by 18 publications

(21 citation statements)

References 102 publications

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“…Finally, in the case of cryopreservation of tissue samples for further cultivation, it is important not only to preserve the viability of all cell subpopulations within the tissue, but also to preserve the structure and composition of the extracellular matrix, tissue architecture and intercellular interactions, preventing clonal selection (Jacob, Ming, and Song, 2020). An even more difficult task is the biobanking of tissue-engineered complex 3D constructs, both devoid of a matrix and based on a synthetic or natural matrix, and there are currently many studies in this direction (Gryshkov et al, 2021;Arutyunyan, Elchaninov, Sukhikh, and Fatkhudinov, 2021;Wang et al, 2022). Herein, we will discuss biobanking of such complex systems, and their potential applications, in more detail.…”

Section: Biobanked Samples Storage and Sharing: Methodology And Appro...mentioning

confidence: 99%

Biobanks as an important tool in modern translational oncology

Petersen

Chudakova

Shabalina

et al. 2022

BioComm

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The creation and use of biobanks is an actively growing field that plays an important role in the development of many branches of biotechnology and biomedicine, including oncology and translational medicine. In this review, based on the analysis of more than 80 Russian and foreign publications, we describe the current state of biobanking and its future perspectives. The diversity of biobanking and the problems arising from it, including the limits of applicability to different types of research, as well as the prospects for development are discussed. The role of biobanks in the study of malignant neoplasms, including rare/orphan diseases, and in the development of new diagnostic and therapeutic approaches, personalised medicine and pre-clinical screening studies, are highlighted.

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Section: Biobanked Samples Storage and Sharing: Methodology And Appro...mentioning

confidence: 99%

Biobanks as an important tool in modern translational oncology

Petersen

Chudakova

Shabalina

et al. 2022

BioComm

View full text Add to dashboard Cite

show abstract

“…Different from slow freezing, vitrification requires rapid cooling of the samples into deep cryogenic temperatures with high concentrations (>5M). 17 By this method, cells or tissues are directly transformed into a glass state without ice nucleation formation. Although with the advantage of avoiding freeze injury, CPA-induced cytotoxicity, high risk of pathogenic agent contamination, and high demand for manipulation skills need to be considered.…”

Section: Cryopreservationmentioning

confidence: 99%

“…However, slow freezing faces the problem of cryo‐induced injury during the formation of extracellular ice. Different from slow freezing, vitrification requires rapid cooling of the samples into deep cryogenic temperatures with high concentrations (>5M) 17 . By this method, cells or tissues are directly transformed into a glass state without ice nucleation formation.…”

Section: Freezing Strategies Of Cryopreservationmentioning

confidence: 99%

“…Scaffolds with a bulky and monolithic nature is another cell delivery system, which consists of films, sponge, electrospun nanofibers, etc 3 . a Compared with microencapsulated cells, which are much closer to free cells, the cryopreservation of the cell delivery scaffolds faces more obstacles, including non‐uniform distribution of CPAs for cells adherent or located deep in the bulk, compromised accuracy of temperature control, and uneven shrinkage or expansion of the matrices, etc 17 . Although with much difficulty, many research studies have demonstrated the possibility of the long‐term cryogenic storage of scaffold‐based cell delivery systems Multiple parameters, such as cell types, properties of the scaffold materials, cell culture conditions before freezing, and CPAs, should be taken into consideration for the successful cryopreservation 32…”

Section: Freezing Strategies Of Cryopreservationmentioning

confidence: 99%

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Freezing biological organisms for biomedical applications

et al. 2022

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Biological organisms play important roles in human health, either in a commensal or pathogenic manner. Harnessing inactivated organisms or living organisms is a promising way to treat diseases. As two types of freezing, cryoablation makes it simple to inactivate organisms that must be in a non‐pathogenic state when needed, while cryopreservation is a facile way to address the problem of long‐term storage challenged by living organism‐based therapy. In this review, we present the latest studies of freezing biological organisms for biomedical applications. To begin with, the freezing strategies of cryoablation and cryopreservation, as well as their corresponding technical essentials, are illustrated. Besides, biomedical applications of freezing biological organisms are presented, including transplantation, tissue regeneration, anti‐infection therapy, and anti‐tumor therapy. The challenges and prospects of freezing living organisms for biomedical applications are well discussed. We believe that the freezing method will provide a potential direction for the standardization and commercialization of inactivated or living organism‐based therapeutic systems, and promote the clinical application of organism‐based therapy.

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“…However, even in the case of rapid sterility evaluation, prolonged storage of the finally packed preparation represents an additional challenge. Current cryopreservation strategies allow successful preservation of cell suspensions, but in the case of 3D scaffold-based systems the typical cell viability post-thaw comprises around 50 % [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Hypothermic Storage of 3D Cultured Multipotent Mesenchymal Stromal Cells for Regenerative Medicine Applications

et al. 2022

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The regulatory requirements in cell processing, in the choice of a biomaterial scaffold and in quality control analysis, have to be followed in the clinical application of tissue-engineered grafts. Confirmation of sterility during quality control studies requires prolonged storage of the cell-based construct. After storage, preservation of the functional properties of the cells is an important prerequisite if the cells are to be used for cell-based tissue therapies. The study presented here shows the generation of 3D constructs based on Wharton’s jelly multipotent mesenchymal stromal cells (WJ-MSCs) and the clinically-acceptable HyaloFast® scaffold, and the effect of two- and six-day hypothermic storage of 3D cell-based constructs on the functional properties of populated cells. To study the viability, growth, gene expression, and paracrine secretion of WJ-MSCs within the scaffolds before and after storage, xeno-free culture conditions, metabolic, qPCR, and multiplex assays were applied. The WJ-MSCs adhered and proliferated within the 3D HyaloFast®. Our results show different viability of the cells after the 3D constructs have been stored under mild (25 °C) or strong (4 °C) hypothermia. At 4 °C, the significant decrease of metabolic activity of WJ-MSCs was detected after 2 days of storage, with almost complete cell loss after 6 days. In mild hypothermia (25 °C) the decrease in metabolic activity was less remarkable, confirming the suitability of these conditions for cell preservation in 3D environment. The significant changes were detected in gene expression and in the paracrine secretion profile after 2 and 6 days of storage at 25 °C. The results presented in this study are important for the rapid transfer of tissue engineering approaches into clinical applications.

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Cryopreservation of Tissue-Engineered Scaffold-Based Constructs: from Concept to Reality

Cited by 18 publications

References 102 publications

Biobanks as an important tool in modern translational oncology

Biobanks as an important tool in modern translational oncology

Freezing biological organisms for biomedical applications

Hypothermic Storage of 3D Cultured Multipotent Mesenchymal Stromal Cells for Regenerative Medicine Applications

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