Cryopreservation of wild Shih (Artemisia herba-alba Asso.) shoot-tips by encapsulation-dehydration and encapsulation-vitrification

Sharaf, Sarab A.; Shibli, Rida A.; Kasrawi, M. A.; Baghdadi, Savinaz H.

doi:10.1007/s11240-011-0054-0

Cited by 34 publications

(26 citation statements)

References 22 publications

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“…in LN. Sucrose is the most frequently used carbon source in culture media for plant growth and is also widely used as an osmotic in cryoprotection to stabilize cell membranes and maintain tissue turgor [31]. In this study, incubation in PVS2 had a positive effect on survival of cryopreserved apices; however, survival decreased with increased exposure duration to the solution.…”

Section: Discussionmentioning

confidence: 61%

Cryopreservation of Cat’s Claw (<i>Uncaria tomentosa</i>)

Pérez¹,

Guillén²,

Ramos³

et al. 2015

AJPS

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Uncaria tomentosa presents tomentum that resembles cat's claws, hence its common name, is a plant that produces various secondary metabolites that are traditionally used in alternative medicine. The natural distribution of this species has been affected by indiscriminate harvesting from its habitat. In the present research, cryopreservation (liquid nitrogen, LN, −196˚C) was evaluated as an option for ex situ conservation of this species. The following techniques were evaluated: vitrification and encapsulation-dehydration of apices, vitrification of cell suspensions, and seed desiccation and vitrification. Preculture conditions and exposure times to LS and PVS2 were evaluated. Apex survival was the highest (82%) with preculture in 0.25 M sucrose followed by incubation for 20 and 30 min in LS and PVS2, respectively, prior to cooling in LN. The encapsulation-dehydration technique was evaluated by using sucrose preculture and different capsule moisture contents. Survival of apices cooled in LN was not significantly different between treatments and varied from 31.8% to 52.9% for capsule moisture contents between 22.7% and 20.3%. For cell suspensions precultured in 0.5 M sucrose, cell multiplication and formation of calli with very good appearance were observed in 61.1% of the cultures following vitrification. For cryopreservation of seeds, germination was 89.5% using the desiccation technique and 67.6% to78.1% using vitrification.

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Section: Discussionmentioning

confidence: 61%

Cryopreservation of Cat’s Claw (<i>Uncaria tomentosa</i>)

Pérez¹,

Guillén²,

Ramos³

et al. 2015

AJPS

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“…Os explantes encapsulados restantes (144) foram transferidos para solução crioprotetora composta por meio MS suplementado com 0,5M de sacarose (SOLIMAN, 2013 adaptado) sob agitação a 100rpm (SHARAF et al, 2012) onde permaneceram por 24 e 48 horas a 25±2°C na ausência de luz.…”

Section: Methodsunclassified

“…Após o encapsulamento, foram pesadas para determinar o peso da massa fresca (MF) e, depois de cada período de imersão em solução crioprotetora (24 e 48 horas) e de desidratação em câmara de fluxo laminar (0, 2 e 4 horas), foram colocadas em estufa a 90ºC por 16 horas (SHARAF et al, 2012) e pesadas para determinação da massa seca (MS). A umidade (U) foi determinada pela seguinte fórmula U=(MF-MS)/MF*100.…”

Section: Methodsunclassified

Encapsulamento, crioproteção e desidratação na capacidade regenerativa de ápices caulinares de Genipa americana

et al. 2015

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“…fragrantissima (Younis, 2012), Artemisia herba-alba Asso. (Sharaf et al, 2012) and Teucrium Polium L. (Rabba'a et al, 2012).…”

Section: In Vitro Conservationmentioning

confidence: 99%

In Vitro Conservation and Cryopreservation of Medicinal and Aromatic Plants : A Review = حفظ النباتات الطبية و العطرية داخل الأنابيب و التجميد بالنيتروجين السائل : مراجعة

Shibli¹,

Al-Qudah²,

Al‐Abdallat³

2015

JJAS

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Medicinal and aromatic plants are those containing special chemical components that enable them to relief pain, release pleasant aromas, and improve food flavors. Meanwhile, medicinal and aromatic plants are under real jeopardy due to the uncontrolled collection of these plants as a result of being extensively used in herbal medicine and food industry. So conservation action for these natural resources is of high priority. Plants conserved under in situ conservation conditions are exposed to natural disasters, pests and pathogens in addition to the fluctuating government policies. Also ex situ conservation is very difficult to be applied as adequate samples have to be collected for the conservation of genetic diversity. In vitro conservation is offering a strong and a multi package of techniques that do so good when other conservation methods are not feasible. Slow growth conservation is a very simple in vitro technique based on reducing the growth rates of the tissue cultured plant for short or mid-term storage and yet increasing the intervals between subcultures. In this conservation type, several techniques are used separately or in combinations to slow down the growth rate of the stored explants, such as, addition of elevated levels of osmotic agents or ABA in addition to storage under minimal growth conditions such as low temperature and dark incubation. Cryopreservation is another conservation technique that is usually described as the most reliable tool for long-term storage of plant germplasm and reported to be advantageous over most other conservation methods in terms of simplicity, applicability to a wide range of genotypes and ability to maintain the genetic stability of plant material. Thanks to the use of cryopreservation techniques, such as, encapsulation-dehydration, vitrification, encapsulation-vitrification, and droplet-vitrification, many medicinal plant species are conserved indefinitely for the next generations.

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Cryopreservation of wild Shih (Artemisia herba-alba Asso.) shoot-tips by encapsulation-dehydration and encapsulation-vitrification

Cited by 34 publications

References 22 publications

Cryopreservation of Cat’s Claw (<i>Uncaria tomentosa</i>)

Cryopreservation of Cat’s Claw (<i>Uncaria tomentosa</i>)

Encapsulamento, crioproteção e desidratação na capacidade regenerativa de ápices caulinares de Genipa americana

In Vitro Conservation and Cryopreservation of Medicinal and Aromatic Plants : A Review = حفظ النباتات الطبية و العطرية داخل الأنابيب و التجميد بالنيتروجين السائل : مراجعة

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Cryopreservation of wild Shih (Artemisia herba-alba Asso.) shoot-tips by encapsulation-dehydration and encapsulation-vitrification

Cited by 34 publications

References 22 publications

Cryopreservation of Cat’s Claw (&lt;i&gt;Uncaria tomentosa&lt;/i&gt;)

Cryopreservation of Cat’s Claw (&lt;i&gt;Uncaria tomentosa&lt;/i&gt;)

Encapsulamento, crioproteção e desidratação na capacidade regenerativa de ápices caulinares de Genipa americana

In Vitro Conservation and Cryopreservation of Medicinal and Aromatic Plants : A Review = حفظ النباتات الطبية و العطرية داخل الأنابيب و التجميد بالنيتروجين السائل : مراجعة

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Cryopreservation of Cat’s Claw (<i>Uncaria tomentosa</i>)

Cryopreservation of Cat’s Claw (<i>Uncaria tomentosa</i>)