Cryopreservation Techniques for Conservation of Tropical Horticultural Species Using Various Explants

Malik, Sunil; Chaudhury, Rekha

doi:10.1007/978-981-13-3669-0_19

Cited by 19 publications

(3 citation statements)

References 24 publications

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“…Cryopreservation procedures have been established and implemented for many vegetatively propagated genebank collections [ 22 , 29 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ]. Cryopreservation protocols have been described for grapevines, dating back to as early as the 1990s ( Table 1 ); however, Vitis cryo-storage has been challenging and has not been widely used within genebanks.…”

Section: Introductionmentioning

confidence: 99%

Grapevine Shoot Tip Cryopreservation and Cryotherapy: Secure Storage of Disease-Free Plants

Bettoni

Marković

et al. 2021

Plants

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Grapevine (Vitis spp.) is one of the most economically important temperate fruit crops. Grapevine breeding programs require access to high-quality Vitis cultivars and wild species, which may be maintained within genebanks. Shoot tip cryopreservation is a valuable technique for the safe, long-term conservation of Vitis genetic resources that complements traditional field and in vitro germplasm collections. Vitis is highly susceptible to virus infections. Virus-free plants are required as propagation material for clonally propagated germplasm, and also for the global exchange of grapevine genetic resources. Shoot tip cryotherapy, a method based on cryopreservation, has proven to be effective in eradicating viruses from infected plants, including grapevine. This comprehensive review outlines/documents the advances in Vitis shoot tip cryopreservation and cryotherapy that have resulted in healthy plants with high regrowth levels across diverse Vitis species.

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Section: Introductionmentioning

confidence: 99%

Grapevine Shoot Tip Cryopreservation and Cryotherapy: Secure Storage of Disease-Free Plants

Bettoni

Marković

et al. 2021

Plants

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“…As for the tropical and subtropical fruits, most of them have nutritional, medicinal, and industrial properties [ 25 , 26 ]. Their production has been increasing significantly faster than that of temperate fruits in recent years ( Figure 1 ).…”

Section: Tropical and Subtropical Ornamental And Fruit Plantsmentioning

confidence: 99%

Cryopreservation of Endangered Ornamental Plants and Fruit Crops from Tropical and Subtropical Regions

Kaviani

Kulus

2022

Biology

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Horticultural crops comprise various economic species extending from fruits, nuts, vegetables, spices and condiments, ornamentals, aromatic, and medicinal plants. Ornamental and fruit plants are produced mainly for their nutritional and aesthetic values, respectively. Unfortunately, many tropical and subtropical species are in danger of extinction because of climate change and (a)biotic stresses. It is imperative to preserve the germplasms of these species for the present and future genetic improvement programs. Cryopreservation, i.e., maintenance of tissues at the ultralow temperature of liquid nitrogen, is a promising long-term preservation technique, alternative to seed or in vitro banks, which can be applied for both vegetatively and generatively (through seeds) propagated crops, including those with recalcitrant seeds. It is a technology of choice not only for the preservation of plant biodiversity but also for virus elimination in the proficient administration of large-scale micropropagation. The main advantages of cryopreservation are the lowering of in vitro culture expenditures, needed space, contamination risk, and operator errors. However, tropical species are temperature delicate and one of the foremost challenging issues is preconditioning treatments that stimulate physiological reactions to sufficiently enhance tolerance to dehydration and cryogenic procedures. In recent years, several cryopreservation methods based on encapsulation-vitrification, droplet-vitrification, the use of aluminum cryo-plates, and cryo-mesh have been established. Combined cryo-techniques, gene/DNA conservation, as well as studies on perceiving bio-molecular events and exploring the multistage process from the beginning to end of cryopreservation are receiving more emphasis. The development of cryobiomics delivers a conceptual framework to assess the significance of cell signaling mechanisms on cellular functions, the influence of cryoinjury factors on sample viability, and the implications for genetic stability following cryo-storage. The aim of this mini-review article is to provide a succinct synthesis of the developed cryogenic procedures and their use for the storage and exchange of genetic resources of tropical and subtropical horticultural crops, particularly fruit crops and ornamental plants under the threat of extinction.

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“…There are more than 800 papers on shoot tip cryopreservation using vitrification solutions in the literature [18,19]. In addition, several vitrification solutions with different compositions have been reported, but most of the vitrification-based protocols use only a few key solutions [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Vitrification Solutions for Plant Cryopreservation: Modification and Properties

Zámečnı́k

Faltus

Bilavčík

2021

Plants

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Many plants cannot vitrify themselves because they lack glassy state-inducing substances and/or have high water content. Therefore, cryoprotectants are used to induce vitrification. A cryoprotectant must have at least the following primary abilities: high glass-forming property, dehydration strength on a colligative basis to dehydrate plant cells to induce the vitrification state, and must not be toxic for plants. This review introduces the compounds used for vitrification solutions (VSs), their properties indicating a modification of different plant vitrification solutions, their modifications in the compounds, and/or their concentration. An experimental comparison is listed based on the survival or regeneration rate of one particular species after using more than three different VSs or their modifications. A brief overview of various cryopreservation methods using the Plant Vitrification Solution (PVS) is also included. This review can help in alert researchers to newly introduced PVSs for plant vitrification cryoprotocols, their properties, and the choice of their modifications in the compounds and/or their concentration.

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Cryopreservation Techniques for Conservation of Tropical Horticultural Species Using Various Explants

Cited by 19 publications

References 24 publications

Grapevine Shoot Tip Cryopreservation and Cryotherapy: Secure Storage of Disease-Free Plants

Grapevine Shoot Tip Cryopreservation and Cryotherapy: Secure Storage of Disease-Free Plants

Cryopreservation of Endangered Ornamental Plants and Fruit Crops from Tropical and Subtropical Regions

Vitrification Solutions for Plant Cryopreservation: Modification and Properties

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