Cryopreserved rabbit amniotic membrane alleviated inflammatory response and fibrosis following experimental strabismus surgery in rabbits

Chun, Bo Young; Rhiu, Soolienah

doi:10.1371/journal.pone.0187058

Cited by 7 publications

(1 citation statement)

References 32 publications

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“…Here, the time required for the IL-1β concentration to peak was longer than the time required for TNF-α and VEGF to peak. 13 In addition to promoting endometrial tissue repair and maintaining a balance of immune activity in the uterine cavity, IL-1β was found to promote a healthy intrauterine environment and normal receptivity of the endometrium. 14 Although its concentration in the study group was lower than that in the control group due to inhibition of TNF-α and VEGF in the early stage, IL-1β promotes self-secretion through the IL-1β/IL-1R1 pathway.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Freeze-Dried Amnion Grafts on Cytokines in Uterine Exudates Following Hysteroscopic Adhesiolysis of Severe Intrauterine Adhesions

Wang¹,

Duan²,

Li³

et al. 2022

IJGM

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Background Intrauterine adhesions (IUAs) are a benign uterine disorder that results in intrauterine adhesions and scarring. This study was conducted to assess the effects of freeze-dried amnion grafts on the concentrations of adhesion-related cytokines in uterine exudates following hysteroscopic adhesiolysis of IUAs. Materials and Methods This study was a prospective randomized controlled trial. Thirty patients who underwent hysteroscopic adhesiolysis for severe IUAs were enrolled. They were randomly divided into a study group (with freeze-dried amnion graft treatment after surgery, N = 15) and a control group (without a graft, N = 15). After complete separation of adhesions, a Foley balloon containing a freeze-dried amnion graft was inserted into the uterine cavity in the study group, while a Foley balloon with no graft was placed in the control group. Enzyme-linked immunosorbent assays were performed to test adhesion-related cytokine concentrations in uterine exudates, including IL-1β, TNF-α, and VEGF, at different time intervals after surgery. Second-look hysteroscopy was conducted three months after the surgery. On the basis of the American Fertility Society (AFS) scoring system, the level of adhesions was evaluated during hysteroscopy. Results The postoperative volume of the uterine exudates in the study group was significantly lower than that of the control group ( P < 0.05). The adhesion-related cytokine concentrations of TNF-α, VEGF, and IL-1β significantly increased after surgery in both groups. Their concentrations were significantly lower in the study group than in the control group ( P < 0.05). Moreover, IL-1β exhibited a long-lasting effect in the study group. AFS scores and readhesion rates were significantly lower in the study group than in the control group after hysteroscopic adhesiolysis of severe IUAs. Conclusion The application of freeze-dried amnion grafts is beneficial to the postoperative recovery of patients with severe IUAs and may reduce the readhesion rate after hysteroscopic adhesiolysis by lowering adhesion-related cytokines, including TNF-α, VEGF, and IL-1β.

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Section: Discussionmentioning

confidence: 99%