Cryopreserved storage of clonal germplasm in the USDA National Plant Germplasm System

Jenderek, Maria M.; Reed, Barbara M.

doi:10.1007/s11627-017-9828-3

Cited by 101 publications

(39 citation statements)

References 70 publications

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“…Considering the long-term preservation, the decline of recovery was noteworthy, but recovery was still 58%, i.e. more than 40% which is considered adequate for long term preservation (Forsline et al 1998;Reed et al 1998;Jenderek and Reed 2017). Previously, the decline of regrowth rates after 5 years in cryostorage was reported for birch (Betula pendula Roth) (Ryynänen 1999).…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Rantala

Kaseva

Karhu

et al. 2019

Plant Cell Tiss Organ Cult

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Cryopreservation of dormant buds can be a feasible method for long term preservation of clonally propagated woody plants.In the present study, dormant buds of blackcurrant (Ribes nigrum L) cultivar Mortti were cryopreserved. Twig segments from greenhouse and field grown plants were cooled at their natural moisture content at the rate of 0.17 °C min −1 from zero to −38 °C, immersed in liquid nitrogen and stored in liquid nitrogen vapor. The post-cryopreservation regrowth of buds was evaluated in vitro and viability of recovered plants was tested in vivo. The estimated recovery rate for buds thawed after 1-6 days in cryostorage was 86% and 66% for outdoor and greenhouse grown buds, respectively. Microplants adequate in size and quality grew successfully in vivo and plants produced berries in field. After 4 years in cryostorage, the estimated recovery rate for outdoor buds was 58%. According to results from 1 to 6 days cryostorage, the tested dormant bud protocol turned to be applicable for cryopreservation of the blackcurrant cultivar Mortti. However, concerning the long-term preservation the decline of recovery is important to consider in the future experiments. Key messageThe cryopreservation of dormant buds combined with in vitro recovery provides a feasible tool for conservation of plant genetic resources of blackcurrant.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Rantala

Kaseva

Karhu

et al. 2019

Plant Cell Tiss Organ Cult

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“…The most commonly used cryopreservation techniques are vitrification, encapsulation dehydration, controlled rate cooling, and dormant bud preservation (Benson 2008;Benelli et al 2013;Jenderek and Reed 2017). Vitrification is a physical process where solution solidifies into a metastable glass at very low temperatures and avoids crystallisation (Sakai et al 2008).…”

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confidence: 99%

Cryopreservation enables long-term conservation of critically endangered species Rubus humulifolius

et al. 2019

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Ex situ storage plays an important role in the conservation of plant biodiversity. Cryopreservation at ultra-low temperatures (− 196 °C) is the only long-term ex situ preservation method for plant species that cannot be stored in seed banks. In the present study, we developed a cryopreservation protocol for micropropagated Rubus humulifolius (Rosaceae) plants representing currently critically endangered population of the species in Finland. Abscisic acid (ABA) has been found to increase the freezing tolerance of several plant species. Thus, we studied the effect of a 10-day pretreatment with 0, 2 or 4 mg/l ABA in comparison to freshly dissected buds. We also studied how the duration of in vitro subculture affects cryopreservation result. The ABA pretreatment had divergent effect on control and cryopreserved buds: the regeneration of non-cryopreserved control buds increased from 51% to 70%, 90% or 87% while the regeneration of cryopreserved buds decreased from 52% to 35%, 6% or 9% after 0, 2 or 4 mg/l ABA pre-treatments, respectively. Buds from plants subcultured for 1 month had 63% survival, which, however, decreased to 29% or nil% after 2 or 4 months subculture. The regenerated plants were successfully transferred from in vitro to in vivo conditions in common garden. Growing in garden is needed for future restoration of the species in wild. Cryostorage and other ex situ conservation actions carried out in botanical gardens may be of increasing importance as a tool to maintain plant biodiversity in the future.

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“…С использованием метода дроплет-витрификации созданы криоколлекции многочисленных образцов следующих культур (табл. 1): банана в Бельгии (Panis et al, , 2009; лука и чеснока в Южной Корее (Kim et al, 2006(Kim et al, , 2009 и Германии (Keller et al, 2016); картофеля в Перу (Panta et al, 2015;Vollmer et al, 2016Vollmer et al, , 2017, США (Bamberg et al, 2016;Jenderek, Reed, 2017) и России (Shvachko, Gavrilenko, 2011;Ukhatova et al, 2017b;Gavrilenko et al, 2018); малины и ежевики в США (Reed, DeNoma, 2012;Jenderek, Reed, 2017 Метод Cryo-plate, сочетающий приемы инкапсуляции-дегидратации и дроплет-витрификации, является самым новым (Yamamoto et al, 2011(Yamamoto et al, , 2012(Yamamoto et al, , 2015Valle Arizaga et al, 2017). В этом методе апексы микропобегов in vitro растений прикрепляют тонким слоем альгината кальция к алюминиевым крио-пластинам, обрабатывают их растворами LS и PVS, а затем быстро погружают криопластины в жидкий азот (Yamamoto et al, 2011).…”

Section: Cryoconservation Methods For Vegetatively Propagated Cropsunclassified

“…В частности, в NCGR Corvallis (National Clonal Germplasm Repository Corvallisколлекции Национального репозитория США в Корваллисе) в in vitro системе сохраняется 222 образца рода Fragaria, 248 образцов рода Rubus, 25 образцов рода Ribes и 135 образцов рода Sorbus (Annual Report..., 2014). В NCGRP (National Center for Genetic Resources Preservation, Fort Collins, США) на длительном криохранении находятся: 2155 образцов рода Malus (спящие почки), 219 образцов рода Pyrus (спящие почки и апексы побегов in vitro растений), 280 образцов рода Fragaria (апексы побегов in vitro растений), 187 -рода Rubus (апексы побегов in vitro растений), 79 образцов рода Ribes (спящие почки и апексы побегов in vitro растений) и 42 образца рода Vaccinium (апексы побегов in vitro растений) (Jenderek, Reed, 2017).…”

Section: заключениеunclassified

An Integrated Approach to Creating Scab-Resistant Apple: Phytopathological Testing and Marker-Assisted Selection

Супрун¹,

Насонов²,

Lоbodina³

et al. 2018

PBT&B

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Cryopreserved storage of clonal germplasm in the USDA National Plant Germplasm System

Cited by 101 publications

References 70 publications

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Cryopreservation enables long-term conservation of critically endangered species Rubus humulifolius

An Integrated Approach to Creating Scab-Resistant Apple: Phytopathological Testing and Marker-Assisted Selection

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