Cryostorage of Citrus Embryogenic Cultures

Pérez, Rosa Miriam Blanco

doi:10.1007/978-94-017-3030-3_25

Cited by 9 publications

(4 citation statements)

References 52 publications

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“…Konservasi in vitro merupakan salah satu alternatif dari konservasi ex-situ. Strategi konservasi in vitro dapat diterapkan untuk penyimpanan plasma nutfah dalam jangka pendek, menengah dan panjang dalam kondisi steril (Keller et al, 2006;Pérez, 2000). Konservasi jangka pendek dan menengah secara in vitro memberikan beberapa keuntungan dibandingkan konservasi di lapangan karena sistem aseptik menjamin stok tanaman yang bebas patogen, menghemat ruang, mengurangi erosi genetik karena pemeliharaan optimal, mengurangi biaya pemeliharaan, mudah diperbanyak secara masal jika mendadak diperlukan, dan mempermudah pertukaran plasma nutfah (Ahmed dan Anjum, 2010;Engelmann, 1991).…”

Section: Abstrakunclassified

Respon Pertumbuhan Kultur In Vitro Jeruk Besar (Citrus maxima (Burm.) Merr.) cv. Nambangan terhadap Osmotikum dan Retardan

Dewi¹,

Jawak²,

Purwoko³

et al. 2015

J Hort Indonesia

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ABSTRACTIn vitro conservation has been applied to many species. However, the suppression of explant growth is essential for extending the duration of conservation. The objective of the research was to study in vitro growth response of pummelo cv. Nambangan to conservation medium containing osmotically active compound (osmoticum) or growth suppressant (retardant). Two sets of experiments were conducted using randomized complete design and replicated three times. In vitro shoot with four leaves from pummelo, namely cultivar Nambangan, were used as the plant materials. The treatment in the first experiment was MS + osmoticum (mannitol 0, 20, 40, and 60 g L-1) and in the second experiment was MS + retardant (paclobutrazol 0, 1, 3 and 5 mg L-1). The resultsindicated that senescence of the leaf was induced by 20, 40, and 60 g L-1of mannitol. The best media in inhibition of growth for pummelo cv. Nambangan was MS + paclobutrazol 1 mg L-1. With this media, plant was inhibited but grew normally with green leaf and root.Keywords: mannitol, minimal growth, paclobutrazol, pummelo ABSTRAKKonservasi in vitro sudah banyak dilakukan pada berbagai spesies. Penghambatan pertumbuhansangat penting bagi lamanya tanaman dapat disimpan. Tujuan penelitian ini adalah mempelajari respon pertumbuhan in vitro pamelo cv. Nambangan terhadap media konservasi mengandung osmotikum atau penghambat pertumbuhan (retardan). Dua percobaan dilakukan terpisah menggunakan rancangan acak lengkap dan diulang 3 kali. Tunas hasil perbanyakan in vitro dengan 4 daun, digunakan sebagai eksplan. Perlakuan pada percobaan pertama adalah MS + osmotikum (mannitol 0, 20, 40, 60 g L-1) dan pada percobaan kedua adalah MS + retardan (paclobutrazol 0, 1, 3,5 mg L-1). Hasil menunjukkan bahwa daun mengalami senesen oleh perlakuan mannitol. Media yang direkomendasikan untuk konservasi pamelo cv. Nambangan adalah MS + paclobutrazol 1 mg L-1. Dengan media tersebut pertumbuhan dihambat, tetapi tetap normal, berakar dengan daun tetap hijau.Kata kunci: mannitol, pertumbuhan minimal, paclobutrazol, pamelo

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Section: Abstrakunclassified

Respon Pertumbuhan Kultur In Vitro Jeruk Besar (Citrus maxima (Burm.) Merr.) cv. Nambangan terhadap Osmotikum dan Retardan

Dewi¹,

Jawak²,

Purwoko³

et al. 2015

J Hort Indonesia

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“…Concentrated intracellular solute is a pre-requisite for successful cryopreservation and can be achieved with the following methods (Table 2), either individually or in combination [54][55][56][57]. Table 2 Methods to dehydrate/concentrate intracellular solute for cryopreservation.…”

Section: Cryopreservation Methods To Reduce Water Contentmentioning

confidence: 99%

Cryopreservation for Tree Species with Recalcitrant Seeds: the Avocado Case

O’Brien¹,

Hiti-Bandaralage²,

Folgado³

et al. 2020

Preprint

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Recent developments in the cryopreservation space has increased the trend in germplasm collections established through cryopreserved in vitro material. Cryopreservation of recalcitrant seeds through embryos and embryonic axes, is not uncommon. Tropical and sub-tropical plants are not acclimated to the cold season, therefore have no in-built natural resilience to the cold. Also, larger seeds from trees, such as avocado (Persea americana Mill.), mango (Mangifera indica) and durian (Durio zibethinus L.) are sensitive to desiccation, chilling and freezing stress, making them unsuitable for seed banking or cryopreservation. Alternatively, as seeds do not carry the same genetic make-up as the mother plant, especially in the context of woody rainforest species of which the cross-pollination is dominant; seed conservation does not serve the purpose of germplasm preservation. Other plant material and methods are needed for these plants to be successfully stored in liquid nitrogen (LN). One such method commonly used is shoot-tip cryopreservation which ensures the clonal fidelity of germplasm. There are many problems when using shoot tips of tropical recalcitrant-seeded species. These include: 1) the toxic effects of cryoprotective agents towards structural integrity; 2) optimum developmental stage for success and 3) oxidative stress associated with excision injury leading to necrosis triggering cell death and hindering regeneration for the shoot tips in culture. A pre-requisite for any cryopreservation system is the availability of an established tissue culture regeneration platform. This review will outline conservation strategies for avocado with special emphasis on attempts and improvements made in the cryopreservation space for storing this horticulturally important crop ‘avocado’ at ultra-low temperatures.

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“…Concentrated intracellular solute is a pre-requisite for successful cryopreservation and can be achieved with the following methods ( Table 3 ) either individually or in combination [ 50 , 51 , 52 , 53 ].…”

Section: Plant Cryopreservation Of Somatic Embryos and Shoot Tipsmentioning

confidence: 99%

Cryopreservation of Woody Crops: The Avocado Case

O’Brien

Hiti-Bandaralage

Folgado

et al. 2021

Plants

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Recent development and implementation of crop cryopreservation protocols has increased the capacity to maintain recalcitrant seeded germplasm collections via cryopreserved in vitro material. To preserve the greatest possible plant genetic resources globally for future food security and breeding programs, it is essential to integrate in situ and ex situ conservation methods into a cohesive conservation plan. In vitro storage using tissue culture and cryopreservation techniques offers promising complementary tools that can be used to promote this approach. These techniques can be employed for crops difficult or impossible to maintain in seed banks for long-term conservation. This includes woody perennial plants, recalcitrant seed crops or crops with no seeds at all and vegetatively or clonally propagated crops where seeds are not true-to-type. Many of the world’s most important crops for food, nutrition and livelihoods, are vegetatively propagated or have recalcitrant seeds. This review will look at ex situ conservation, namely field repositories and in vitro storage for some of these economically important crops, focusing on conservation strategies for avocado. To date, cultivar-specific multiplication protocols have been established for maintaining multiple avocado cultivars in tissue culture. Cryopreservation of avocado somatic embryos and somatic embryogenesis have been successful. In addition, a shoot-tip cryopreservation protocol has been developed for cryo-storage and regeneration of true-to-type clonal avocado plants.

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Cryostorage of Citrus Embryogenic Cultures

Cited by 9 publications

References 52 publications

Respon Pertumbuhan Kultur In Vitro Jeruk Besar (Citrus maxima (Burm.) Merr.) cv. Nambangan terhadap Osmotikum dan Retardan

Respon Pertumbuhan Kultur In Vitro Jeruk Besar (Citrus maxima (Burm.) Merr.) cv. Nambangan terhadap Osmotikum dan Retardan

Cryopreservation for Tree Species with Recalcitrant Seeds: the Avocado Case

Cryopreservation of Woody Crops: The Avocado Case

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