Cryostorage of white cachama (Piaractus orinoquensis) sperm: Effects on cellular, biochemical and ultrastructural parameters

Medina‐Robles, Víctor M.; Sandoval‐Vargas, Leydy Y.; Suárez-Martínez, Roger Oswaldo; Gómez-Ramírez, Edwin; Guaje-Ramírez, Diana Nataly; Cruz‐Casallas, Pablo E.

doi:10.1016/j.aqrep.2023.101477

Cited by 2 publications

(1 citation statement)

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“…In addition, a study by Amidi et al (288) identified spermatozoa susceptibility to ROS injury, highlighting the need for antioxidants during cryopreservation to help the cellular antioxidant defence system to maintain the balance. An additional study on the cryostorage of white cachama (Piaractus orinoquensis) sperm presented by Medina-Robles et al (289) demonstrated significantly affected motility and viability of spermatozoa after 24-hour cryostorage, with no significant difference across the year of storage as well as for other variables, such as pH, motility duration, and total antioxidant capacity. On the other hand, ATP content and DNA integrity were stable for 24 hours and 1 month, but drastically decreased when stored for 6 and 12 months, in addition to the observed substantial ultrastructural damage mostly at the head level depicted in Figure 20.…”

Section: Cryopreservationmentioning

confidence: 98%

Cryogenic Media in Biomedical Applications: Current Advances, Challenges, and Future Perspectives

MOKBEL,

KODRESKO,

GHAZAL

et al. 2023

In Vivo

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This paper explores the crucial role of cryogenic mediums in driving breakthroughs within the biomedical sector. The objective was to investigate, critically discuss, and present the current knowledge and state-of-the-art practices, along with the challenges and perspectives of the most common applications. Through an extensive literature review, this work aims to supplement existing research, offering a comprehensive and up-to-date understanding of the subject. Biomedical research involving cryogenic mediums is advancing on multiple fronts, including the development of advanced medical technologies, clinical treatments for life-threatening conditions, high-quality biospecimen preservation, and antimicrobial interventions in industrial food processing. These advances open new horizons and present cutting-edge opportunities for research and the medical community. While the current body of evidence showcases the impressive impact of cryogenic mediums, such as nitrogen, helium, argon, and oxygen, on

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Section: Cryopreservationmentioning

confidence: 98%

Cryogenic Media in Biomedical Applications: Current Advances, Challenges, and Future Perspectives

MOKBEL,

KODRESKO,

GHAZAL

et al. 2023

In Vivo

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Influence of Dietary Fatty Acids on Fish Sperm Tolerance to Cryopreservation

Figueroa Villalobos,

Pereira,

Pérez‐Atehortúa

et al. 2024

Reviews in Aquaculture

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To meet growing fish demand, aquaculture must develop sustainable, cost‐effective, and high‐performance fish feed formulations for industry advancement. Plant‐based lipids are a viable alternative to forage fish ingredients in finfish diets. However, most plant‐derived lipids lack long‐chain polyunsaturated fatty acids (LC‐PUFA) like EPA and DHA, which are nutritionally essential for fish. This review summarizes recent findings on the nutritional aspects of fish brood stock and sperm quality, emphasizing the effects of fatty acids on reproductive outcomes. Studies indicate that substituting fish oil with plant oils alters the fatty acid profiles in fish tissues and gametes, potentially compromising sperm quality, cryopreservation success, and progeny viability. Sperm membrane lipids, including EPA, DHA, and ARA, are vital for motility and fertilization rates post‐thaw. Additionally, cryopreservation has been linked to molecular and epigenetic alterations in sperm, which may negatively affect offspring quality. The role of mRNA and noncoding RNA in regulating sperm function and embryonic development is well known, and thus any damage to these molecules as a consequence of cryopreservation might induce long‐term effects on offspring. Proteomic analyses reveal that cryopreservation can lead to significant protein loss in sperm, diminishing their functional and fertilization capabilities. Optimizing cryoprotectant protocols and freezing techniques is crucial to reduce damage, while dietary fatty acids play a key role in preserving sperm quality during cryopreservation stress. Further research with omic technologies is crucial to fully understand sperm tolerance to cryopreservation, which will improve reproductive outcomes and enhance sustainability and quality in aquaculture.

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Cryostorage of white cachama (Piaractus orinoquensis) sperm: Effects on cellular, biochemical and ultrastructural parameters

Cited by 2 publications

References 95 publications

Cryogenic Media in Biomedical Applications: Current Advances, Challenges, and Future Perspectives

Cryogenic Media in Biomedical Applications: Current Advances, Challenges, and Future Perspectives

Influence of Dietary Fatty Acids on Fish Sperm Tolerance to Cryopreservation

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