Cryotherapy of cutaneous leishmaniasis caused by Leishmania major in BALB/c mice: A comparative experimental study

Shaddel, Minoo; Sharifi, Iraj; Karvar, Mehran; Keyhani, Alireza; Baziar, Zahra

doi:10.4103/0972-9062.234625

Cited by 11 publications

(6 citation statements)

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“…Parasitic infections are a major threat to humans and animals, leading to serious diseases (22,23). The control strategy for Fasciola infection is currently based on using antihelmintic drugs.…”

Section: Discussionmentioning

confidence: 99%

Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

Homayouni,

Shaddel,

Ebrahimi

et al. 2023

Ann Mil Health Sci Res

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Background: Fasciolosis is a global parasitic disease caused by liver trematodes such as Fasciola hepatica, affecting various mammals, including sheep, goats, cattle, pigs, and humans worldwide. Cathepsin L (Cat L) found in the life stages of F. gigantica is associated with the pathology of the fluke. Given the detrimental impact of fasciolosis on animal health and production, along with reports of drug resistance and concerns regarding drug residues in animal products and the environment, there has been an increasing emphasis on developing a potent epitope-based vaccine to combat this infection. Objectives: The present study aimed at bioinformatically analyzing the characteristics of Cat L of F. gigantica newly encysted juvenile (FgNEJ) to identify potential epitopes for constructing an effective vaccine against the infection. Methods: The Cat L of FgNEJ was analyzed using several reliable bioinformatics tools. Results: Analysis of the second structure of the Cat L amino acid sequence demonstrated that the alpha-helix, random coil, and extended strand ratios were 77 (23.62%), 164 (50.31%), and 85 (26.07%), respectively. Analysis of B-cell, T-cell, and MHC epitopes predicted on the sequence suggested Cat L of FgNEJ as an ideal target for vaccine design. Conclusions: The current study introduces significant information on Cat L of FgNEJ, as well as some promising epitopes that can be used as strong candidates in studies involved in developing a vaccine against fasciolosis.

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Section: Discussionmentioning

confidence: 99%

Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

Homayouni,

Shaddel,

Ebrahimi

et al. 2023

Ann Mil Health Sci Res

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“… 27 Cryotherapy is also used to treat cutaneous leishmaniasis lesions by direct application of liquid nitrogen or, in resource-limited settings, carbon dioxide solids at temperatures lethal to parasites. 31 , 35 , 36 , 48 While these therapies have been deemed safe and effective with few side effects, cure rates vary based on a multitude of factors such as parasite species and lesion size. With these limitations in mind, combination therapies with chemotherapeutics may still be recommended, but these methods alone are especially favorable for patients who cannot receive traditional chemotherapies such as pregnant or immunosuppressed patients.…”

Section: Current Therapies and Field-deployable Therapiesmentioning

confidence: 99%

Field-Deployable Treatments For Leishmaniasis: Intrinsic Challenges, Recent Developments and Next Steps

Pacheco-Fernandez,

Markle,

Verma

et al. 2023

RRTM

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Leishmaniasis is a neglected tropical disease endemic primarily to low- and middle-income countries, for which there has been inadequate development of affordable, safe, and efficacious therapies. Clinical manifestations of leishmaniasis range from self-healing skin lesions to lethal visceral infection with chances of relapse. Although treatments are available, secondary effects limit their use outside the clinic and negatively impact the quality of life of patients in endemic areas. Other non-medicinal treatments, such as thermotherapies, are limited to use in patients with cutaneous leishmaniasis but not with visceral infection. Recent studies shed light to mechanisms through which Leishmania can persist by hiding in cellular safe havens, even after chemotherapies. This review focuses on exploring the cellular niches that Leishmania parasites may be leveraging to persist within the host. Also, the cellular, metabolic, and molecular implications of Leishmania infection and how those could be targeted for therapeutic purposes are discussed. Other therapies, such as those developed against cancer or for manipulation of the ferroptosis pathway, are proposed as possible treatments against leishmaniasis due to their mechanisms of action. In particular, treatments that target hematopoietic stem cells and monocytes, which have recently been found to be necessary components to sustain the infection and provide a safe niche for the parasites are discussed in this review as potential field-deployable treatments against leishmaniasis.

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“…Most frequently used cryogen is liquid nitrogen (N2) at a temperature of -196 °C, for cryotherapy of cutaneous lesions. 9 Freezing can be done either by using a cotton tip applicator or by the cryo-spray gun. 10 Gupta et al 11 conducted a study to evaluate the treatment by topical adapalene 0.1% gel versus cryotherapy treatment in patients presenting with plantar warts and found time for clearance of the wart in each group to be 36.71±19.24 days versus 52.17±30.06 days respectively.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Topical 0.1% Adapalene Gel Versus Cryotherapy Treatment in Patients With Plantar Warts

Mahboob¹,

Ahmed²,

Rahman

et al. 2022

J Ayub Med Coll Abbottabad

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Background: Plantar wart is a common viral infection of the plantar surface of the foot. Multiple treatment modalities are available but there is no definitive management option. The aim of this study is to compare topical adapalene gel 0.1% with cryotherapy in patients presenting with plantar warts in terms of time taken for complete clearance of the lesions. Methods: The study was conducted at the department of Dermatology, PNS Shifa Hospital, Karachi from 28th April to 28th October 2020. Eighty-four patients with plantar warts who fulfilled the inclusion and exclusion criteria were included in the study. Approval from the institutional ethical review committee was sought and written informed consent was taken from all the patients. Patients were divided into two groups, A (Adapalene 0.1% gel) and B (Cryotherapy) of 42 patients each. Adapalene gel was applied twice daily under occlusion at home and cryotherapy was done at the clinic after every two weeks. Patients were followed weekly from the onset of treatment and days taken for complete clearance of plantar warts were noted. Both the groups were compared for the outcome, i.e., time taken for complete clearance of lesions. Results: The mean time for complete clearance of plantar warts in group A was 35.619±3.154 days and in group B, it was 50.404±3.178 days. Conclusion: Adapalene gel 0.1% used for the treatment of plantar warts helped in complete clearance of lesions faster than cryotherapy.

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Cryotherapy of cutaneous leishmaniasis caused by Leishmania major in BALB/c mice: A comparative experimental study

Abstract: Liquid N cryotherapy is superior to CO cryotherapy, and it can be an effective method for controlling L. major infection. Further investigations are essential to find optimal number of treatment sessions and time intervals.

Cited by 11 publications

References 0 publications

Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

Field-Deployable Treatments For Leishmaniasis: Intrinsic Challenges, Recent Developments and Next Steps

Comparison of Topical 0.1% Adapalene Gel Versus Cryotherapy Treatment in Patients With Plantar Warts

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