Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries

Antinori, Monica; Licata, Emanuele; Dani, G; Cerusico, F; Versaci, C.; Antinori, S.

doi:10.1016/s1472-6483(10)60766-3

Cited by 210 publications

(121 citation statements)

References 51 publications

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“…Only metaphase II (MII) oocytes were selected for vitrification. A modified vitrification method was adopted for cryopreservation of the mature oocytes [Chian et al 2005;Antinori et al 2007]. Briefly, the oocytes were placed into equilibration medium, containing 7.5% (v/v) ethylene glycol (EG) and 7.5% (v/v) dimethyl sulfoxide (DMSO), at room temperature for 5 min, and then the oocytes were transferred to vitrification medium, containing 15% (v/v) EG, 15% (v/v) DMSO, and 0.50 mol/L sucrose at room temperature for 45 to 60 s. Two or three oocytes were loaded on a McGill Cryoleaf (Medicult Company, Denmark) and plunged immediately in liquid nitrogen for vitrification and then for storage.…”

Section: Methodsmentioning

confidence: 99%

Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Song

Sun

Jin

et al. 2011

Systems Biology in Reproductive Medicine

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The development of an effective oocyte cryopreservation system will have a significant impact on the clinical practice of reproductive medicine. However, the important option of emergency oocyte cryopreservation has yet to be well documented. In this report, we review the cases of 15 women with male partners who were diagnosed with nonobstructive azoospermia and for whom testicular sperm extraction on the day of oocyte retrieval failed. Emergency oocyte vitrification was performed and after two months, the vitrified oocytes were warmed and the surviving oocytes inseminated with frozen-thawed donor sperm by intracytoplasmic sperm injection (ICSI). A total of 117 mature oocytes from the 15 women were vitrified and warmed. The post-warming survival rate was 84.6% (99/117), and the fertilization rate following ICSI was 83.8% (83/99). We selected 30 embryos for transfer to 15 patients, 8 of whom became pregnant. The clinical pregnancy rate was 53.3% (8/15) and the implantation rate was 30.0% (9/30). Nine healthy live births resulted from 8 pregnancies. These results indicate that emergency oocyte vitrification is an effective rescue technique that can be applied clinically with acceptable pregnancy and live birth rates when testicular sperm extraction from the male partner failed on the day of oocyte retrieval. These results also highlight another important option for oocyte cryopreservation through the use of vitrification technology.

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Section: Methodsmentioning

confidence: 99%

Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Song

Sun

Jin

et al. 2011

Systems Biology in Reproductive Medicine

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show abstract

“…With use of this procedure, improved rates of postwarming survival, fertilization, implantation, pregnancy, and live birth are reported (21)(22)(23)(24)(25)(26)(27)(28)(29).…”

mentioning

confidence: 99%

Ultrastructural evaluation of human metaphase II oocytes after vitrification: closed versus open devices

Bonetti¹,

Cervi²,

Tomei³

et al. 2011

Fertility and Sterility

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“…Embryos may pass rapidly through the dangerous zone (temperature range from +20 to -20 o C), which decreases the risk of chilling injury. This method has been applied to various species for embryo cryopreservation, including the cow (Chian et al, 2004), rabbit (Hochi et al, 2004), buffalo (Muenthaisong et al, 2007), pig (Du et al, 2007) and human (Antinori et al, 2007).…”

Section: Cryotop (Also Called Minimum-volume Cooling)mentioning

confidence: 99%

Oocyte and embryo cryopreservation – state of art and recent developments in domestic animals

Gajda¹,

Smorąg²

2009

J. Anim. Feed Sci.

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During the past few decades, significant progress in cryopreservation of mammalian oocytes and embryos has been observed. Transfer of cryopreserved embryos or oocytes resulted in live offspring in at least 25 species. So far, two major methods have been used for oocyte and embryo cryopreservation: conventional slow-rate freezing and vitrification. This review summarizes the progress in cryopreservation of mammalian oocytes and embryos that has been achieved by modifying oocyte/embryo susceptibility to cryopreservation or vitrification technology through such techniques as solid-surface vitrification, cryoloop, microdrop, cryotop, electron microscopy grids, nylon mesh, open pulled straw method or removal of lipids, addition of antifreeze protein or cytoskeletonstabilizing agents, cholesterol or liposomes, centrifugation prior to cryopreservation or application of high hydrostatic pressure. In conclusion, the vitrification method opened new perspectives in cryopreservation of embryos and oocytes, both for in vitro fertilized and somatic nuclear transfer. Authors believe that new cryopreservation procedures which modify the susceptibility of oocytes and embryos to cryopreservation will gain importance as a major tool in mammalian gamete and embryo cryopreservation.

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Cryotop vitrification of human oocytes results in high survival rate and healthy deliveries

Cited by 210 publications

References 51 publications

Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Clinical outcome of emergency egg vitrification for women when sperm extraction from the testicular tissues of the male partner is not successful

Ultrastructural evaluation of human metaphase II oocytes after vitrification: closed versus open devices

Oocyte and embryo cryopreservation – state of art and recent developments in domestic animals

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