Cryphonectria parasitica isolates of the same vegetative compatibility type display different rates of transfer of CHV1 hypovirus

Zamora, Pedro García; Martín, A. B.; Dueñas, M.; Martín, Ricardo San

doi:10.1007/s10658-015-0727-3

Cited by 8 publications

(5 citation statements)

References 35 publications

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“…A high rate of transmission was expected, as mitoviruses FcMV1 and FcMV2-2 are associated with the mitochondria that the offspring inherit from the parents [17]. The high transmission rates of mycoviruses are consistent with those reported for asexual spores of other fungal species such as Ustilaginoidea virens [26] and Epichloë festucae [27] whereas for other pathogens variable rates of virus transmission were observed, e.g., Heterobasidion annosum [13] and CHV1 hypovirus infecting Chryphonectria parasitica [28][29][30]. In some species, usually ascomycetes, there appear to be barriers to the transmission of viruses during sexual reproduction and the formation of sexual spores [27].…”

Section: Discussionsupporting

confidence: 76%

Vertical Transmission of Fusarium circinatum Mitoviruses FcMV1 and FcMV2-2 via Microconidia

Romeralo

Bezos

Martínez‐Álvarez

2018

Forests

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Pine Pitch Canker disease, caused by the pathogenic fungus Fusarium circinatum, affects conifer species worldwide. However, the virulence of the pathogen may be affected by the presence of mycoviruses. The aim of this laboratory-based study was to investigate the probability and rate of transmission of F. circinatum mitoviruses FcMV1 and FcMV2-2 via microconidia. Ten isolates of mitovirus-infected F. circinatum were subcultured to produce a total of 100 single-spore colonies (ten replicates per isolate). The total RNA and cDNA obtained from each spore isolate (monosporic culture) were amplified by PCR with specific primers for detection of F. circinatum mitoviruses FcMV1 and FcMV2-2. The mitoviruses were detected in a high percentage of the individual spore isolates (between 60% and 100% depending on the fungal isolate). However, the probability of transmission was not statistically significantly associated with either the F. circinatum isolate or the viral strain. A high proportion of transmission via microconidia is critical for development of a biological control program against Pine Pitch Canker (PPC) disease in forests. However, further studies are needed to establish the effect of these mitoviruses on the virulence of F. circinatum.

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Section: Discussionsupporting

confidence: 76%

Vertical Transmission of Fusarium circinatum Mitoviruses FcMV1 and FcMV2-2 via Microconidia

Romeralo

Bezos

Martínez‐Álvarez

2018

Forests

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“…dianthi isolates. In addition to the vic genes that regulate hyphal anastomosis, the host genetic background has been shown to affect horizontal virus transmission (Cortesi et al, 2001 ; Choi et al, 2012 ; Zamora et al, 2015 ). Although all race 2 isolates are in VCG 0021, we have evidence that some isolates in a same VCG show a reduced compatibility, that is, they can anastomose with a few but not all the isolates in the VCG (Gómez-Lama Cabanás and Pérez-Artés, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Mycovirus Fusarium oxysporum f. sp. dianthi Virus 1 Decreases the Colonizing Efficiency of Its Fungal Host

Torres-Trenas

Prieto

Cañizares

et al. 2019

Front. Cell. Infect. Microbiol.

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Mycoviruses that induce hypovirulence in phytopathogenic fungi are interesting because their potential use as biological control agents of the plant diseases caused by their fungal hosts. The recently identified chrysovirus Fusarium oxysporum f. sp. dianthi virus 1 (FodV1) has been associated to the induction of hypovirulence in Fusarium oxysporum f. sp. dianthi, the forma specialis of F. oxysporum that causes vascular wilt in carnation (Dianthus caryophyllus). In this work, we have used confocal laser scanner microscopy and two isogenic GFP-labeled strains of F. oxysporum f. sp. dianthi infected (V+) and not infected (V−) with the Fusarium oxysporum f. sp. dianthi virus 1, respectively, to analyze the effect of mycovirus FodV1 on the plant colonization pattern of its fungal host. Results demonstrate that FodV1-viral infection affects the speed and spatial distribution of fungal colonization into the plant. Initial stages of external root colonization were similar for both strains, but the virus-free strain colonized the internal plant tissues faster than the virus-infected strain. In addition, other differences related to the specific zone colonized and the density of colonization were observed between both F. oxysporum f. sp. dianthi strains. The hyphae of both V− and V+ strains progressed up through the xylem vessels but differences in the number of vessels colonized and of hyphae inside them were found. Moreover, as colonization progressed, V− and V+ hyphae propagated horizontally reaching the central medulla but, while the virus-free strain V− densely colonized the interior of the medulla cells, the virus-infected strain V+ appeared mainly in the intercellular spaces and with a lower density of colonization. Finally, the incidence of FodV1-viral infections in a collection of 221 isolates sampled between 2008 and 2012 in the geographic area where the originally infected isolate was obtained has been also analyzed. The very low (<2%) incidence of viral infections is discussed here. To the best of our knowledge, this work provides the first microscopic evidence about the effect of a hypovirulence-inducing mycovirus on the pattern of plant colonization by its fungal host.

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“…In addition, several studies have shown that vegetative compatibility is not an impossible obstacle to overcome. For example, Hamid et al [ 59 ] found a novel mycovirus in S. sclerotiorum that was identified and described as the first +ssRNA mycovirus capable of spreading across the vegetative compatibility barrier [ 55 , 60 , 61 ]. Brusini and Robin found that although the transmission of CHV1 between vic strains was difficult in plates, it was much easier when the strains were inoculated in chestnuts [ 62 ].…”

Section: Mycoviruses: a Natural Source Of Fungal Hypovirulencementioning

confidence: 99%

New Insights on the Integrated Management of Plant Diseases by RNA Strategies: Mycoviruses and RNA Interference

Bocos-Asenjo

Niño‐Sánchez

Ginésy

2022

IJMS

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RNA-based strategies for plant disease management offer an attractive alternative to agrochemicals that negatively impact human and ecosystem health and lead to pathogen resistance. There has been recent interest in using mycoviruses for fungal disease control after it was discovered that some cause hypovirulence in fungal pathogens, which refers to a decline in the ability of a pathogen to cause disease. Cryphonectria parasitica, the causal agent of chestnut blight, has set an ideal model of management through the release of hypovirulent strains. However, mycovirus-based management of plant diseases is still restricted by limited approaches to search for viruses causing hypovirulence and the lack of protocols allowing effective and systemic virus infection in pathogens. RNA interference (RNAi), the eukaryotic cell system that recognizes RNA sequences and specifically degrades them, represents a promising. RNA-based disease management method. The natural occurrence of cross-kingdom RNAi provides a basis for host-induced gene silencing, while the ability of most pathogens to uptake exogenous small RNAs enables the use of spray-induced gene silencing techniques. This review describes the mechanisms behind and the potential of two RNA-based strategies, mycoviruses and RNAi, for plant disease management. Successful applications are discussed, as well as the research gaps and limitations that remain to be addressed.

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Cryphonectria parasitica isolates of the same vegetative compatibility type display different rates of transfer of CHV1 hypovirus

Cited by 8 publications

References 35 publications

Vertical Transmission of Fusarium circinatum Mitoviruses FcMV1 and FcMV2-2 via Microconidia

Vertical Transmission of Fusarium circinatum Mitoviruses FcMV1 and FcMV2-2 via Microconidia

Mycovirus Fusarium oxysporum f. sp. dianthi Virus 1 Decreases the Colonizing Efficiency of Its Fungal Host

New Insights on the Integrated Management of Plant Diseases by RNA Strategies: Mycoviruses and RNA Interference

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