Cryptic diversity and multiple origins of the widespread mayfly species group<i>Baetis rhodani</i>(Ephemeroptera: Baetidae) on northwestern Mediterranean islands

Bisconti, Roberta; Canestrelli, Daniele; Tenchini, Roberta; Belfiore, Carlo; Buffagni, Andrea; Nascetti, Giuseppe

doi:10.1002/ece3.2465

Cited by 21 publications

(28 citation statements)

References 69 publications

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“…In addition, the coexistence of multiple mitochondrial haplotypes can lead to sequence variability in a single organism (heteroplasmy) and can impede correct species identification (Kang, Kim, Park, Kim, & Kim, ; Magnacca & Brown, ). Finally, other demographic processes, for example, migration driven by major climate changes such as glaciation events or bottleneck events, can have a huge impact on the variability of the mtDNA, which was reported among other orders for mayflies, caddisflies and stoneflies (Williams, Ormerod, & Bruford, ; Lehrian, Pauls, & Haase, ; Kubow, Robinson, Shama, & Jokela, ; Bálint et al., ; Vuataz et al., ; Theissinger et al., ; Bisconti et al., ; Gattolliat et al., ). Despite such potential complications, there is evidence from many large‐scale studies that DNA barcoding is a highly effective tool for species identification.…”

Section: Discussionmentioning

confidence: 91%

“…() (Appendix S4). However, because several former species in this genus have been synonymized with B. rhodani (Gattolliat & Sartori, ; Müller‐Liebenau, ), there is a strong possibility that there may be more than one valid species (Bauernfeind & Soldán, ; Bisconti et al., ; Rutschmann, Gattolliat, Hughes, Sartori, & Monaghan, ). Another species with apparently difficult taxonomy is B. vernus Curtis, 1834, which was represented by four clusters in a paraphyletic group with B. liebenauae Keffemuller, 1974 that was previously considered as belonging to the B. vernus species group (Savolainen, Drotz, Hoffsten, & Saura, ; Ståhls & Savolainen, ) based on allozyme electrophoresis.…”

Section: Discussionmentioning

confidence: 99%

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A DNA barcode library for Germany′s mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera)

Morinière

Hendrich

Balke

et al. 2017

Molecular Ecology Resources

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Mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera) are prominent representatives of aquatic macroinvertebrates, commonly used as indicator organisms for water quality and ecosystem assessments. However, unambiguous morphological identification of EPT species, especially their immature life stages, is a challenging, yet fundamental task. A comprehensive DNA barcode library based upon taxonomically well-curated specimens is needed to overcome the problematic identification. Once available, this library will support the implementation of fast, cost-efficient and reliable DNA-based identifications and assessments of ecological status. This study represents a major step towards a DNA barcode reference library as it covers for two-thirds of Germany's EPT species including 2,613 individuals belonging to 363 identified species. As such, it provides coverage for 38 of 44 families (86%) and practically all major bioindicator species. DNA barcode compliant sequences (≥500 bp) were recovered from 98.74% of the analysed specimens. Whereas most species (325, i.e., 89.53%) were unambiguously assigned to a single Barcode Index Number (BIN) by its COI sequence, 38 species (18 Ephemeroptera, nine Plecoptera and 11 Trichoptera) were assigned to a total of 89 BINs. Most of these additional BINs formed nearest neighbour clusters, reflecting the discrimination of geographical subclades of a currently recognized species. BIN sharing was uncommon, involving only two species pairs of Ephemeroptera. Interestingly, both maximum pairwise and nearest neighbour distances were substantially higher for Ephemeroptera compared to Plecoptera and Trichoptera, possibly indicating older speciation events, stronger positive selection or faster rate of molecular evolution.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

A DNA barcode library for Germany′s mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera)

Morinière

Hendrich

Balke

et al. 2017

Molecular Ecology Resources

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“…High intraspecific variation (>3% divergence in cox1 ) is a well‐established observation in the case of Asellus (Sworobowicz et al., ). Three BOLD‐OTUs were assigned to Baetis rhodani (Ephemeroptera), which is also reflected in the incomplete taxonomy of this species complex (Bisconti et al., ; Williams, Ormerod, & Bruford, ).…”

Section: Methodsmentioning

confidence: 99%

Metabarcoding of freshwater invertebrates to detect the effects of a pesticide spill

et al. 2017

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Biomonitoring underpins the environmental assessment of freshwater ecosystems and guides management and conservation. Current methodology for surveys of (macro)invertebrates uses coarse taxonomic identification where species-level resolution is difficult to obtain. Next-generation sequencing of entire assemblages (metabarcoding) provides a new approach for species detection, but requires further validation. We used metabarcoding of invertebrate assemblages with two fragments of the cox1 "barcode" and partial nuclear ribosomal (SSU) genes, to assess the effects of a pesticide spill in the River Kennet (southern England). Operational taxonomic unit (OTU) recovery was tested under 72 parameters (read denoising, filtering, pair merging and clustering). Similar taxonomic profiles were obtained under a broad range of parameters. The SSU marker recovered Platyhelminthes and Nematoda, missed by cox1, while Rotifera were only amplified with cox1. A reference set was created from all available barcode entries for Arthropoda in the BOLD database and clustered into OTUs. The River Kennet metabarcoding produced matches to 207 of these reference OTUs, five times the number of species recognized with morphological monitoring. The increase was due to the following: greater taxonomic resolution (e.g., splitting a single morphotaxon "Chironomidae" into 55 named OTUs); splitting of Linnaean binomials into multiple molecular OTUs; and the use of a filtration-flotation protocol for extraction of minute specimens (meiofauna). Community analyses revealed strong differences between "impacted" vs. "control" samples, detectable with each gene marker, for each major taxonomic group, and for meio- and macrofaunal samples separately. Thus, highly resolved taxonomic data can be extracted at a fraction of the time and cost of traditional nonmolecular methods, opening new avenues for freshwater invertebrate biodiversity monitoring and molecular ecology.

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“…Speciation in mayflies has been associated with other kinds of obstacles to gene flow. For example, species‐level divergence in groups has been associated with changes in sea levels, continental uplift and glaciation (Bisconti et al., ; Sekiné, Hayashi, & Tojo, ; Theissinger et al., ). One of the few desert mayfly species studied genetically, Callibaetis americanus , represented a diverse cryptic species complex, with significant restriction of gene flow and considerable CO1 divergence between populations in spring habitats (Stutz, Shiozawa, & Evans, ).…”

Section: Discussionmentioning

confidence: 99%

Evolutionary divergence in freshwater insects with contrasting dispersal capacity across a sea of desert

et al. 2017

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Summary Arid landscapes pose arguably one of the greatest challenges to dispersal of aquatic insects, and may drive speciation in taxa with low dispersal potential. We investigated genetic divergence in aquatic insects with high and low dispersal potential between two regions within the Australian arid zone. We used two dragonfly species to infer patterns for strong‐dispersing species, and mayfly species from two genera to represent weak‐dispersing species. Based on dispersal‐related traits of the taxa, we predicted that dragonflies would show little divergence between and within the two geographical regions, while mayflies would show evidence of genetic isolation, with divergence timing associated with aridification in Australia. Samples were collected from perennial pools in ephemeral stream networks in central and western Australia. The two study regions are separated by approximately 1,500 km of predominantly dune desert. Collected insects were sequenced for one mitochondrial and one nuclear marker. We investigated spatial distribution of haplotypes, estimated divergence dates for identified mayfly lineages, and performed phylogenetic reconstruction to investigate relationships with known congeners. Both dragonfly species showed evidence of recent or ongoing gene flow between the central and western Australian study regions. In contrast, mayflies showed evidence of ancient, but not recent, gene flow between regions, with species‐level CO1 divergence within regions. We found 11 previously unknown putative mayfly species, that if confirmed could double the known diversity within these genera in Australia. Timing of divergence events for mayflies coincided with the development of the Australian arid zone, and phylogenetic relationships are similar to divergence patterns found in other Australian arid taxa. The findings of this study suggest that aridification is more likely to be a driver of diversification in taxa with low dispersal potential than in those with high dispersal potential. This is because effective aerial dispersal is integral for maintaining gene flow when aquatic connections are lost between distant populations, and reduced gene flow can promote genetic divergence.

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Cryptic diversity and multiple origins of the widespread mayfly species groupBaetis rhodani(Ephemeroptera: Baetidae) on northwestern Mediterranean islands

Cited by 21 publications

References 69 publications

A DNA barcode library for Germany′s mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera)

A DNA barcode library for Germany′s mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera)

Metabarcoding of freshwater invertebrates to detect the effects of a pesticide spill

Evolutionary divergence in freshwater insects with contrasting dispersal capacity across a sea of desert

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