Crypticity and functional distribution of the membrane associated α‐<scp>L</scp>‐fucosidase of human sperm

Venditti, Jennifer J.; Donigan, Katherine A.; Bean, Barry

doi:10.1002/mrd.20666

Cited by 26 publications

(39 citation statements)

References 38 publications

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“…As outlined in the introduction, there is abundant evidence to implicate biochemical recognition of fucose residues in the reproduction of many species. These functions may include direct roles in sperm-oocyte recognition and binding, and the arrival of sperm-associated fucosidase in the otherwise fucosidase-deficient ooplasm may serve as an important regulatory trigger for further embryonic development [14,15]. Herein we have reported the presence of sperm-associated fucosidase in fishes.…”

Section: Discussionmentioning

confidence: 83%

“…Substantial evidence in the literature supports roles for carbohydrates during fertilization [1][2][3][4][5][6][7][8][9][10]. Research over the past two decades has led to the identification of sperm associated -L-fucosidases in humans [11][12][13][14][15] rats [16][17][18], Drosophila [19][20][21], ascidians [22], Unio elongatulus [23], chimpanzees [24], bulls [25] and Syrian hamsters (Venditti, Swann, and Bean, in revision), but not yet in fishes. Additionally, L-fucose has been shown to be important during fertilization [26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

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Fucosidases of Sperm and Milt in Darters (Percidae: Etheostomatini)

Venditti

Mendelson²,

Bean³

2009

TORSJ

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Darters (Percidae: Etheostomatini) offer attractive opportunities for analysis of the evolution of reproductive barriers and the molecular basis of fertilization. Here we report some basic characteristics of the sperm of darters, and, to our knowledge, the first report of sperm associated -L-fucosidase in fishes. Fluorometric enzyme assays using 4-methylumbelliferyl-alpha-L-fucopyranoside [4-MU-fuc] as a substrate provided direct quantification of enzyme activity in both the soluble and cellular fractions of milt from all six species tested. Further analysis using SDS-PAGE and Western blotting revealed that fish sperm and milt contain -L-fucosidase isoforms with molecular weights similar to those reported for human semen. Fertilization of darter ova by conspecific sperm, as assessed by development of the embryo to the 4 cell stage, was inhibited by pretreatment of sperm with the fucosidase-specific competitive inhibitor DFJ, implicating the sperm fucosidase in fertilization and/or early embryogenesis. Identification of sperm associated -Lfucosidase in darters is consistent with the importance of carbohydrates during fertilization.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Fucosidases of Sperm and Milt in Darters (Percidae: Etheostomatini)

Venditti

Mendelson²,

Bean³

2009

TORSJ

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“…However, studies that have attempted to relate mutations in the ID3 gene to fertility in cattle were not found. The second gene, located 462 kb upstream from BTA-49769-no-rs, was Bos taurus fucosidase, alpha-L-1, tissue (FUCA1), which is broadly distributed over the membrane systems of human sperm and has a role in the intimate species signature interactions between the sperm and oocyte (Venditti et al, 2007). Even though FUCA1 was found to play a clear role in fertility, the evidence reported suggests a role in male rather than female fertility; although both are correlated (Berry et al, 2011).…”

Section: Genetic Parametersmentioning

confidence: 99%

Genome-wide associations for fertility traits in Holstein–Friesian dairy cows using data from experimental research herds in four European countries

Berry

Bastiaansen

Veerkamp

et al. 2012

Animal

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Genome-wide association studies for difficult-to-measure traits are generally limited by the sample population size with accurate phenotypic data. The objective of this study was to utilise data on primiparous Holstein-Friesian cows from experimental farms in Ireland, the United Kingdom, the Netherlands and Sweden to identify genomic regions associated with traditional measures of fertility, as well as a fertility phenotype derived from milk progesterone profiles. Traditional fertility measures investigated were days to first heat, days to first service, pregnancy rate to first service, number of services and calving interval (CI); post-partum interval to the commencement of luteal activity (CLA) was derived using routine milk progesterone assays. Phenotypic and genotypic data on 37 590 single nucleotide polymorphisms (SNPs) were available for up to 1570 primiparous cows. Genetic parameters were estimated using linear animal models, and univariate and bivariate genome-wide association analyses were undertaken using Bayesian stochastic search variable selection performed using Gibbs sampling. Heritability estimates of the traditional fertility traits varied from 0.03 to 0.16; the heritability for CLA was 0.13. The posterior quantitative trait locus (QTL) probabilities, across the genome, for the traditional fertility measures were all ,0.021. Posterior QTL probabilities of 0.060 and 0.045 were observed for CLA on SNPs each on chromosome 2 and chromosome 21, respectively, in the univariate analyses; these probabilities increased when CLA was included in the bivariate analyses with the traditional fertility traits. For example, in the bivariate analysis with CI, the posterior QTL probability of the two aforementioned SNPs were 0.662 and 0.123. Candidate genes in the vicinity of these SNPs are discussed. The results from this study suggest that the power of genome-wide association studies in cattle may be increased by sharing of data and also possibly by using physiological measures of the trait under investigation.Keywords: genome-wide association, fertility, cow, progesterone ImplicationsTraditional genetic selection for fertility is made difficult by the low heritability of traditional fertility phenotypes. This limitation can be minimised by increasing the heritability of the trait(s) or by using genomic information that explains a large proportion of the genetic variation in fertility. In the present study, both approaches were investigated. Heritability estimates greater than some traditional fertility traits were observed for the post-partum commencement of luteal activity quantified using milk progesterone concentration. Specific regions of the genome associated with fertility were also observed, albeit they only explained a small proportion of the total genetic variance in the traits.

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“…Therefore, the presence of active glycosidases at a neutral pH in the OF could have potential roles in different events related to sperm reservoir formation, sperm capacitation, final oocyte maturation, gamete interaction, and early embryo development. It has already been proposed that glycosidases could be involved in the control of polyspermy (Miller et al 1993, Velasquez et al 2007, sperm-oviductal epithelial cells interaction (Lefebvre et al 1997), sperm-zona pellucida binding (Miller et al 1993, Matsumoto et al 2002, Venditti et al 2007, sperm capacitation (Taitzoglou et al 2007), or dispersion of cumulus cells (Takada et al 1994). All these events take place in the oviduct and have been the aim of studies to increase our knowledge about the molecular basis of reproductive functions, but, surprisingly, we lack of precise studies about the activities of the different glycosidases in the OF.…”

Section: Introductionmentioning

confidence: 99%

Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle

Carrasco

Romar

Sánchez³

et al. 2008

Reproduction

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Sperm-oocyte binding and gamete-oviductal epithelium interactions are carbohydrate-mediated events occurring in the oviductal fluid (OF). Thus, knowledge about the activities of glycosidases (enzymes catalyzing hydrolytic cleavage of terminal sugar residues) in this milieu would help us understand the molecular mechanisms involved in these events. This work was carried out to investigate the glycosidase activity, protein content, and volume of OF collected from gilts and sows. Oviducts were classified into four phases of the estrous cycle (early follicular, late follicular, early luteal, and late luteal) based on the appearance of the ovaries. OF was aspirated, centrifuged, measured for volume, and frozen until assay. Substrates conjugated to 4-methylumbelliferyl were used to screen the activities of seven different glycosidases at physiological pH (7.2). a-L-Fucosidase and b-N-acetyl-glucosaminidase activities increased at the late follicular phase to decrease after ovulation. b-D-Galactosidase, a-D-mannosidase, and b-N-acetyl-galactosaminidase showed higher activities at the early follicular phase, which decreased after ovulation. N-Acetyl-neuraminidase and a-D-galactosidase did not show activity at any phase of estrous cycle neither in sows nor in gilts at pH 7.2, although it did at acidic pH (4.4) in the follicular and luteal phase samples. Total protein also changed during the cycle showing the maximum secretion at the late follicular phase (2118.6G200.7 mg/oviduct). The highest volumes of OF were collected from the oviducts at the late follicular phase (50.7G1.3 ml/oviduct). These results indicate that OF from sows and gilts shows glycosidase activity varying throughout the estrous cycle suggesting a role of these enzymes in carbohydrate-mediated events. Reproduction (2008) 136 833-842

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Crypticity and functional distribution of the membrane associated α‐L‐fucosidase of human sperm

Cited by 26 publications

References 38 publications

Fucosidases of Sperm and Milt in Darters (Percidae: Etheostomatini)

Fucosidases of Sperm and Milt in Darters (Percidae: Etheostomatini)

Genome-wide associations for fertility traits in Holstein–Friesian dairy cows using data from experimental research herds in four European countries

Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle

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