Cryptochrome-mediated blue-light signal contributes to lignin biosynthesis in stone cells in pear fruit

Wang, Qi; Gong, Xin; Xie, Zhihua; Qi, Kaijie; Yuan, Kaili; Jiao, Yuru; Pan, Qi; Zhang, Shaoling; Shiratake, Katsuhiro; Tao, Shutian

doi:10.1016/j.plantsci.2022.111211

Cited by 25 publications

(10 citation statements)

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“…Notably, lignin biosynthesis is initiated by the deamidation of phenylalanine and tyrosine, and this is a critical node between primary and secondary plant metabolism (Barros et al., 2022). The role of peroxisomes and light during pear stone cell formation has been reported, but the role of ubiquitin remains unknown (Wang et al., 2022; Wang, Liu, et al., 2021). This finding implies ubiquitination may be involved in stone cell formation.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis reveals that PbMYB61 and PbMYB308 are involved in the regulation of lignin biosynthesis in pear fruit stone cells

Zhu,

Wang,

Jiang

et al. 2023

The Plant Journal

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SUMMARYPear fruit stone cells have thick walls and are formed by the secondary deposition of lignin in the primary cell wall of thin‐walled cells. Their content and size seriously affect fruit characteristics related to edibility. To reveal the regulatory mechanism underlying stone cell formation during pear fruit development and to identify hub genes, we examined the stone cell and lignin contents of 30 ‘Shannongsu’ pear flesh samples and analyzed the transcriptomes of 15 pear flesh samples collected at five developmental stages. On the basis of the RNA‐seq data, 35 874 differentially expressed genes were detected. Additionally, two stone cell‐related modules were identified according to a WGCNA. A total of 42 lignin‐related structural genes were subsequently obtained. Furthermore, nine hub structural genes were identified in the lignin regulatory network. We also identified PbMYB61 and PbMYB308 as candidate transcriptional regulators of stone cell formation after analyzing co‐expression networks and phylogenetic relationships. Finally, we experimentally validated and characterized the candidate transcription factors and revealed that PbMYB61 regulates stone cell lignin formation by binding to the AC element in the PbLAC1 promoter to upregulate expression. However, PbMYB308 negatively regulates stone cell lignin synthesis by binding to PbMYB61 to form a dimer that cannot activate PbLAC1 expression. In this study, we explored the lignin synthesis‐related functions of MYB family members. The results presented herein are useful for elucidating the complex mechanisms underlying lignin biosynthesis during pear fruit stone cell development.

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Section: Resultsmentioning

confidence: 99%

Transcriptome analysis reveals that PbMYB61 and PbMYB308 are involved in the regulation of lignin biosynthesis in pear fruit stone cells

Zhu,

Wang,

Jiang

et al. 2023

The Plant Journal

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“…Then, The plasmids of empty vector and 35S:: PbHB24 fusion constructs were transferred to GV3101 according to its illustration. Agrobacterium cells were injected into the flesh of ‘Dangshansuli’ fruits at 35 Days after full bloom (DAFB) using needleless syringes as discribed previously [ 45 ]. Twenty fruits were injected with each construct.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide characterisation of HD-Zip transcription factors and functional analysis of PbHB24 during stone cell formation in Chinese white pear (Pyrus bretschneideri)

Wang,

Zhang

et al. 2024

BMC Plant Biol

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Background The homodomain-leucine zipper (HD-Zip) is a conserved transcription factor family unique to plants that regulate multiple developmental processes including lignificaion. Stone cell content is a key determinant negatively affecting pear fruit quality, which causes a grainy texture of fruit flesh, because of the lignified cell walls. Results In this study, a comprehensive bioinformatics analysis of HD-Zip genes in Chinese white pear (Pyrus bretschneideri) (PbHBs) was performed. Genome-wide identification of the PbHB gene family revealed 67 genes encoding PbHB proteins, which could be divided into four subgroups (I, II, III, and IV). For some members, similar intron/exon structural patterns support close evolutionary relationships within the same subgroup. The functions of each subgroup of the PbHB family were predicted through comparative analysis with the HB genes in Arabidopsis and other plants. Cis-element analysis indicated that PbHB genes might be involved in plant hormone signalling and external environmental responses, such as light, stress, and temperature. Furthermore, RNA-sequencing data and quantitative real-time PCR (RT-qPCR) verification revealed the regulatory roles of PbHB genes in pear stone cell formation. Further, co-expression network analysis revealed that the eight PbHB genes could be classified into different clusters of co-expression with lignin-related genes. Besides, the biological function of PbHB24 in promoting stone cell formation has been demonstrated by overexpression in fruitlets. Conclusions This study provided the comprehensive analysis of PbHBs and highlighted the importance of PbHB24 during stone cell development in pear fruits.

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“…The flesh of European pear ( Pyrus communis ) fruitlets was used to induce pear calli as previously described (Bai et al, 2019 ). The subculture of pear calli was conducted as previously described (Wang et al, 2022 ). In brief, the pear calli were cultured on solid MS medium supplemented with 1 mg/L 2,4-D and 0.5 mg/L 6-BA at 25 °C in the dark.…”

Section: Methodsmentioning

confidence: 99%

MYB1R1 and MYC2 Regulate ω-3 Fatty Acid Desaturase Involved in ABA-Mediated Suberization in the Russet Skin of a Mutant of ‘Dangshansuli’ (Pyrus bretschneideri Rehd.)

Wang

Liu

et al. 2022

Front. Plant Sci.

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Russeting, a disorder of pear fruit skin, is mainly caused by suberin accumulation on the inner part of the outer epidermal cell layers. ABA was identified as a crucial phytohormone in suberification. Here, we demonstrated that the ABA content in russet pear skin was higher than in green skin. Then, ABA was applied to explore the changes in phenotype and suberin composition coupled with RNA-Seq and metabolomics to investigate the probably regulatory pathway of ABA-mediated suberification. The results showed that ABA treatment increased the expression of ω-3 fatty acid desaturase (FAD) and the content of α-linolenic acid. We identified 17 PbFADs in white pear, and the expression of PbFAD3a was induced by ABA. In addition, the role of PbFAD3a in promoting suberification has been demonstrated by overexpression in Arabidopsis and VIGS assays in the fruitlets. GUS staining indicated that the promoter of PbFAD3a was activated by ABA. Furthermore, MYC2 and MYB1R1 have been shown to bind to the PbFAD3a promoter directly and this was induced by ABA via yeast one-hybrid (Y1H) screening and qRT–PCR. In summary, our study found that ABA induces the expression of MYC2 and MYB1R1 and activates the PbFAD3a promoter, contributing to the formation of russet pear skin. Functional identification of key transcription factors will be the goal of future research. These findings reveal the molecular mechanism of ABA-mediated suberization in the russet skin and provide a good foundation for future studies on the formation of russet skin.

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Cryptochrome-mediated blue-light signal contributes to lignin biosynthesis in stone cells in pear fruit

Cited by 25 publications

References 50 publications

Transcriptome analysis reveals that PbMYB61 and PbMYB308 are involved in the regulation of lignin biosynthesis in pear fruit stone cells

Transcriptome analysis reveals that PbMYB61 and PbMYB308 are involved in the regulation of lignin biosynthesis in pear fruit stone cells

Genome-wide characterisation of HD-Zip transcription factors and functional analysis of PbHB24 during stone cell formation in Chinese white pear (Pyrus bretschneideri)

MYB1R1 and MYC2 Regulate ω-3 Fatty Acid Desaturase Involved in ABA-Mediated Suberization in the Russet Skin of a Mutant of ‘Dangshansuli’ (Pyrus bretschneideri Rehd.)

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