2013
DOI: 10.1128/iai.00554-13
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Cryptococcus neoformans Promotes Its Transmigration into the Central Nervous System by Inducing Molecular and Cellular Changes in Brain Endothelial Cells

Abstract: bCryptococcus spp. cause fungal meningitis, a life-threatening infection that occurs predominately in immunocompromised individuals. In order for Cryptococcus neoformans to invade the central nervous system (CNS), it must first penetrate the brain endothelium, also known as the blood-brain barrier (BBB). Despite the importance of the interrelation between C. neoformans and the brain endothelium in establishing CNS infection, very little is known about this microenvironment. Here we sought to resolve the cellul… Show more

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Cited by 63 publications
(75 citation statements)
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“…The analysis was performed as previously described (Vu et al, 2013). Briefly, X!tandem was used for database searching, and the proteins specifically bound to Sc<Cn MPR1 > were identified with a protein threshold >99.0% and ≄ 3 unique peptides of <0.1 false discovery rate (FDR) using Scaffold 4.…”
Section: Methodsmentioning
confidence: 99%
“…The analysis was performed as previously described (Vu et al, 2013). Briefly, X!tandem was used for database searching, and the proteins specifically bound to Sc<Cn MPR1 > were identified with a protein threshold >99.0% and ≄ 3 unique peptides of <0.1 false discovery rate (FDR) using Scaffold 4.…”
Section: Methodsmentioning
confidence: 99%
“…Vu et al showed that ANXA2 and S100A10 genes were both up-regulated upon contact and ingestion of fungal cells by brain endothelial cells. This up-regulation translated into a decrease in the integrity of the cellular membrane, suggesting that fungal cells enhanced their transmigration into the brain by compromising the underlying cytoskeleton structure as these binding proteins are known to interact with actin (34, 35). …”
Section: Introductionmentioning
confidence: 99%
“…The Rub1 protein is also related to fungal transmigration into the CNS; mutant possessing a null mutation in the putative Rub1 gene exhibited increased transmigration into the brain [13]. Recently, using a spectral counting method, expression of a broad range of host cell proteins involved in cytoskeleton rearrangement, cellular metabolism, intracellular signaling and inflammation were identified to be up-regulated during penetration of the BBB by C. neoformans [14]. Despite these findings, the underlying mechanism of frequent CNS cryptococcosis and the host immune responses during cryptococcal infection remains incompletely understood.…”
Section: Introductionmentioning
confidence: 99%