Cryptomeria japonica-Induced Allergic Conjunctivitis in Mice

Abstract

Japanese cedar pollen (Cryptomeria japonica, Cry j) is the most common allergen causing pollinosis in Japan. However, short ragweed pollen is used commonly as the antigen for experimentally-induced allergic conjunctivitis (EC) and Cry j-induced EC in mice has not been published. We actively immunized BALB/c mice with Cry j, and then performed a challenge with eye drops containing Cry j. We evaluated the early phase and late phase reactions in the conjunctiva, using Evans blue dye leakage and eosinophil infiltration, respectively. Significant inhibition of the early phase reaction was observed following pre-challenge with eye drops that block histamine H1 receptor in the conjunctiva. Thus, Cry j-induced EC appears to represent a suitable model for the study of pollinosis in Japan.

Cyclosporine A Eye Drops Inhibit the Early-Phase Reaction in a Type-I Allergic Conjunctivitis Model in Mice

“…It was reported that anti-histamine is a potent inhibitor of the early-phase reaction. 12,14 Ketotifen instillation showed the most potent inhibitory effect on plasma exudation after single RW challenge. mice.…”

Prevention of allergic conjunctivitis in mice by a rice-based edible vaccine containing modified Japanese cedar pollen allergens

“…The mice also had access to normal chow and drinking water ad libitum. Experimental allergic conjunctivitis due to Japanese cedar pollen was induced by a modified version of a previously described protocol 7. In brief, 0.2 mg of Japanese cedar pollen (Hayashibara, Okayama, Japan) was mixed with alum (2.5 mg) and injected intraperitoneally twice with an interval of 7 days between injections.…”

Systemic overexpression of IFN- and IL-5 exacerbates early phase reaction and conjunctival eosinophilia, respectively, in experimental allergic conjunctivitis

“…Then, an RW challenge was introduced into the left eye with eye-drops, while the right eye was challenged with PBS. Thirty minutes later, the conjunctivas were harvested and weighted, and EB was extracted from the conjunctivas and then measured according to previously described method 12. Data are presented as the differences in the EB levels (μg)/tissue weight (g) between the left and right eyes.…”