Cryptosporidiosis and Immunological Status in Children With Malignant Diseases

Mahdi, Nadham K.; Al-Sadoon, Maysloon A .; Hassan, Genan K.

doi:10.33762/mjbu.2007.48779

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…A study in Addis Ababa, Ethiopia showed infection with the microsporidia parasite using PCR method, the results were 39(16%) positive out of 243 stool samples. This study in slightly disagreed with present study regarding the rate of infection with microsporidia (11).…”

Section: Discussioncontrasting

confidence: 99%

Molecular Characterization of Intestinal Microsporidia in Immunocompromised Patient in THI-QAR Province

Al-Zihiry¹,

Alshuwaili²

2023

PJMHS

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Introduction: Immunocompromised host has one or more defects in the normal defense mechanisms that protect against infectious agents. Microsporidia are group of protozoa characterized via obligate intracellular and spore formation, detected in many hosts. Up till now, 1300 microsporidia species or more, related to 150 genera, were, microsporidiosis caused by microsporidia. Objectives: To conduct Molecular characterization of intestinal microsporidia in immunocompromised patient in Thi-Qar province cases. Patients and Methods: Totally, two hundred stool samples were collected and subdivided into 185 cancer patients and 15 organ transplantation patients. Samples were examined for microsporidiosis by light microscopy smears stained by Chromotrope Kenyon stain and by PCR techniques. Results: using Polymerase chain reaction technique (PCR), microsporidia were detected in eleven sample out of 116 sample that were positive by microscopic examination and Chromotrope Kenyon stain. Positive sample by PCR were sent for sequencing and the result showed that the samples of the current study were similar to three microsporidia, Enterocytozoon bieneusi, Encephalitozoon cuniculi and Encephalitozoon intestinalis. Conclusion: The present study confirmed the presence of Microsporidia infection is among immunocompromised patients. Keyword: Molecular, immunocompromised , microsporidia, PCR

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Section: Discussioncontrasting

confidence: 99%

Molecular Characterization of Intestinal Microsporidia in Immunocompromised Patient in THI-QAR Province

Al-Zihiry¹,

Alshuwaili²

2023

PJMHS

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“…The highest percentage of protozoal infection was recorded among patients with lymphoma (77.27%) followed by (62.26%) among patients with leukemia and (40%) among patients with solid tumors, this difference was not significant (p > 0.05) (Table 3). These results were similar to that of Mahdi et al who reported infection rates of 60.86%, 46.66%, and 44.44% among patients with lymphoma, leukemia, and solid tumors respectively (20). The higher incidence rate of enteric protozoal infections among patients with lymphohematopoietic malignancies could be due to their effects on immunity mechanisms that include mucosal membrane, skin, quantitative and qualitative defect of granulocytes and macrophages (21).…”

Section: Resultssupporting

confidence: 88%

Enteric Protozoal Infections among Children with Cancers Receiving Chemotherapy

khaleel¹,

Al-Mukhtar²

2023

Tikrit J. Pure Sci.

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Objectives: To detect enteric protozoal species in stool samples of children affected by malignancies who are receiving chemotherapy compared to healthy children and to investigate differences in the infection rates among different cancer types. Materials and Methods: This study included 100 children diagnosed with malignancy under chemotherapy attended Ibn-Al-Atheer Teaching Hospital during the period from December 2013 to June 2014. The study also included 50 apparently healthy children as a control group who were age and gender matched to the cancer patients. Children were already diagnosed as having malignant disease by consultant oncologists and receiving chemotherapy. A Questionnaire Form was completed for each child. Stool samples were taken from patients and controls to detect protozoal infections. The stool was tested by direct wet mount preparation, iodine staining, and concentration methods, hot modified Ziehl-Neelson staining method was also used to detect the acid-fast oocysts of Cryptosporidium. Results: Enteric protozoal infection was detected in 60 (60%) of patients compared to 5(10%) of controls (p<0.05). Four protozoal species were observed among patients included G. lamblia representing (24%), C. parvum (19%), E. histolytica/E. dispar (5%), and B. species (5%). Whereas in the control group, included G. lamblia (6%), C. parvum (2%), and B. species (2%). The highest infection rate was found among children with lymphoma (77.27%), followed by (62.26%) and (40%) among patients with leukemia and solid tumors, respectively (p>0.05). Conclusions: The incidence of enteric protozoal infections is higher among cancer children than healthy group and dominates by Giardia lamblia and Cryptosporidium parvum, but other species including E. histolytica/ E. dispar and B. hominis are also found. Patients with lymphohematopoietic malignancies show the highest infection rates.

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