Cryptosporidium

Pinto, Pedro; Ribeiro, Cláudia A.; Kváč, Martin; Tsaousis, Anastasios D.

doi:10.1007/978-3-030-80682-8_7

Cited by 5 publications

(2 citation statements)

References 327 publications

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“…It is the causative agent of cryptosporidiosis, which affects both humans and animals. Two species of Cryptosporidium most commonly cause the disease in humans and other vertebrate hosts: C. parvum and C. hominis (Widmer et al 2020;Pinto et al 2022). Symptoms in humans range from self-limiting nausea and abdominal cramps to life-threatening disease in children under 5 years of age and immunocompromised hosts, such as HIV-positive individuals (Wang et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Presence of Cryptosporidium parvum in pre-washed vegetables from different supermarkets in South East England: A pilot study

Suleiman,

Mavrides,

Maxamhud

et al. 2024

Parasitol Res

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Cryptosporidium is an important water-borne and food-borne parasite with a high burden of disease. This organism has been shown to contaminate various leafy vegetables; however, studies assessing the presence of Cryptosporidium spp in pre-washed and ready-to-eat vegetables are limited. Routine surveillance in the UK revealed a nationwide exceedance of human cases of Cryptosporidium. Therefore, this study aims to assess the presence of this parasite in pre-washed vegetables from supermarkets in the UK. A total of 36 samples were purchased from four different supermarkets. A nested PCR targeting the SSU rRNA was carried out on 24 samples, 58% were PCR-positive for Cryptosporidium. Sanger sequencing confirmed that, of these sequences, 4/24 (17%) produced significant similarities to Cryptosporidium parvum. This study provides evidence for the presence of C. parvum in pre-washed and ready-to-eat vegetables. Future work to identify the point of contamination is required.

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Section: Introductionmentioning

confidence: 99%

“…The parasite is transmitted through the faecal-oral route typically acquired via ingestion of food or water contaminated with oocysts, the transmission form of the parasite. Studies also suggest that Cryptosporidium can also be transmitted through inhalation of oocysts (Pinto et al 2022;Nyangulu et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Presence of Cryptosporidium parvum in pre-washed vegetables from different supermarkets in South East England: A pilot study

Suleiman,

Mavrides,

Maxamhud

et al. 2024

Parasitol Res

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Comparison of in vitro growth characteristics of Cryptosporidium hominis (IdA15G1) and Cryptosporidium parvum (Iowa-IIaA17G2R1 and IIaA18G3R1)

Gunasekera,

Clode,

King

et al. 2023

Parasitol Res

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Cryptosporidium is a major cause of diarrhoeal disease and mortality in young children in resource-poor countries, for which no vaccines or adequate therapeutic options are available. Infection in humans is primarily caused by two species: C. hominis and C. parvum. Despite C. hominis being the dominant species infecting humans in most countries, very little is known about its growth characteristics and life cycle in vitro, given that the majority of our knowledge of the in vitro development of Cryptosporidium has been based on C. parvum. In the present study, the growth and development of two C. parvum isolates (subtypes Iowa-IIaA17G2R1 and IIaA18G3R1) and one C. hominis isolate (subtype IdA15G1) in HCT-8 cells were examined and compared at 24 h and 48 h using morphological data acquired with scanning electron microscopy. Our data indicated no significant differences in the proportion of meronts or merozoites between species or subtypes at either time-point. Sexual development was observed at the 48-h time-point across both species through observations of both microgamonts and macrogamonts, with a higher frequency of macrogamont observations in C. hominis (IdA15G1) cultures at 48-h post-infection compared to both C. parvum subtypes. This corresponded to differences in the proportion of trophozoites observed at the same time point. No differences in proportion of microgamonts were observed between the three subtypes, which were rarely observed across all cultures. In summary, our data indicate that asexual development of C. hominis is similar to that of C. parvum, while sexual development is accelerated in C. hominis. This study provides new insights into differences in the in vitro growth characteristics of C. hominis when compared to C. parvum, which will facilitate our understanding of the sexual development of both species.

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Diagnosis and control of cryptosporidiosis in farm animals

Aboelsoued

Megeed

2022

J Parasit Dis

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Cryptosporidium

Cited by 5 publications

References 327 publications

Presence of Cryptosporidium parvum in pre-washed vegetables from different supermarkets in South East England: A pilot study

Presence of Cryptosporidium parvum in pre-washed vegetables from different supermarkets in South East England: A pilot study

Comparison of in vitro growth characteristics of Cryptosporidium hominis (IdA15G1) and Cryptosporidium parvum (Iowa-IIaA17G2R1 and IIaA18G3R1)

Diagnosis and control of cryptosporidiosis in farm animals

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