Cryptosporidium occurrence in ruminants from the North Pioneer mesoregion of Paraná, Brazil

Abstract

The aim of this study was to investigate the occurrence of Cryptosporidium in cattle and sheep from the North Pioneer mesoregion of the state of Paraná. For this, 317 stool samples were collected from cattle and sheep on 16 properties in six municipalities in the North Pioneer mesoregion of Paraná. For detection of Cryptosporidium species, molecular analysis was performed using nested-PCR techniques targeting the 18S rRNA gene. Of the 37 beef cows and 115 calves analyzed, four (10.8%) and 14 (12.2%), respectively, were positive for Cryptosporidium. Of the 12 cows and 52 calves, one (8.3%) and 14 (26.9%), respectively, were positive for Cryptosporidium; and of the 42 ewes and 59 lambs, six (14.3%) and 12 (20.3%), respectively were positive for Cryptosporidium. Cattle (15.3%) and sheep (17.8%) were both susceptible to infection. All the properties of the municipalities of Assaí, Ibaiti and, Leópolis presented infected animals. The study showed that Cryptosporidium occurs in most municipalities assessed, that dairy calves had a higher risk (Odds Ratio=2,66, p-value=0,018) for infection than beef calves, and that sheep are just as susceptible to infection as are cattle, and that further Cryptosporidium studies are developed.

Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil

“…have described infection by Crypstosporidium in cattle and herd from Brazil (Oliveira et al, 2007;Toledo et al, 2017;Holsback et al, 2018), and these studies reported different prevalence compared to that reported in the present study. The rate of infection with Cryptosporidium spp.…”

“…On the other hand, it is expected that in dairy calves, which are commonly confined, the infection is more common compared to beef calves, which are kept in more open areas (McAllister, Olson, Fletch, Wetzstein, & Entz, 2005), such as the calves included in the present study. In this context, Holsback et al (2018) have reported that dairy calves were 2.66 times more likely to be infected with Cryptosporidium than beef calves from Paraná state, Southern Brazil. Furthermore, the infection rate observed in the present study (21.10%) was higher when compared with that observed in the study conducted by Oliveira et al (2007), among apparently healthy calves (10%) from beef calves in the state of Mato Grosso.…”

“…Despite some molecular researches of Cryptosporidium in cattle have been described in Brazil (Toledo et al, 2017;Holsback et al, 2018), studies concerning Cryptosporidium infection are scarce in the state, with only one published research based on parasitological examination for the detection of oocysts, with no identification at the species level, nor molecular characterization of the protozoan (Oliveira et al, 2007).…”

Multilocus fragment analysis of Cryptosporidium parvum from pre-weaned calves in Colombia

“…Cryptosporidium has been recognised as a cause of diarrhoea in suckling calves in some South American countries (Del Coco et al, 2008;Meireles, 2010;Díaz-Lee et al, 2011;Pulido-Medellín et al, 2014;Holsback et al, 2018). However, the impact of bovine cryptosporidiosis in this continent is not well documented and data on the genetic diversity of C. parvum are limited.…”

Inquérito epidemiológico da infecção por Criptosporidium spp. em ovinos da região metropolitana do Recife e zona da mata de Pernambuco / Epidemiological survey of infection with criptoosporidium spp. in sheep from the metropolitan region of Recife and Pernambuco forest área

“…Dentre as enfermidades parasitárias, destaca-se a criptosporidiose, causada por um protozoário do gênero Cryptosporidium que infecta um número variado de vertebrados, Brazilian Journal of Development, Curitiba, v.7, n.4, p. 36461-36474 apr 2021 comumente presentes em quadros de diarreia em ovinos, principalmente neonatais (VITELA-MENDONZA, 2018;HOLSBACK;LIMA;VIDOTTO, 2018).…”