Cryptosporidium parvum appears to lack a plastid genome

Zhu, Guangxi; Marchewka, Mary J.; Keithly, Janet S.

doi:10.1099/00221287-146-2-315

Cited by 203 publications

(133 citation statements)

References 33 publications

Supporting

Mentioning

132

Contrasting

Order By: Relevance

“…It also appears that certain apicomplexans (e.g. C. parvum) may have secondarily lost their plastid (Zhu et al 2000). Regardless of the precise relationship between the apicomplexan 35 kb DNA p l a n t s c i l i a t e s a p i c o m p l e x a n s a n i m a l s f u n g i k i n e t o p l a s t s g i a r d i a b a c t e r i a origin of the apicoplast origin of plastids origin of mitochondria Figure 1.…”

Section: Discovery Of the Apicomplexan Plastidmentioning

confidence: 99%

Mining thePlasmodiumgenome database to define organellar function: what does the apicoplast do?

Roos

Crawford

Donald

et al. 2002

Phil. Trans. R. Soc. Lond. B

View full text Add to dashboard Cite

Apicomplexan species constitute a diverse group of parasitic protozoa, which are responsible for a wide range of diseases in many organisms. Despite differences in the diseases they cause, these parasites share an underlying biology, from the genetic controls used to differentiate through the complex parasite life cycle, to the basic biochemical pathways employed for intracellular survival, to the distinctive cell biology necessary for host cell attachment and invasion. Different parasites lend themselves to the study of different aspects of parasite biology: Eimeria for biochemical studies, Toxoplasma for molecular genetic and cell biological investigation, etc. The Plasmodium falciparum Genome Project contributes the first large-scale genomic sequence for an apicomplexan parasite. The Plasmodium Genome Database (http://PlasmoDB. org) has been designed to permit individual investigators to ask their own questions, even prior to formal release of the reference P. falciparum genome sequence. As a case in point, PlasmoDB has been exploited to identify metabolic pathways associated with the apicomplexan plastid, or 'apicoplast'-an essential organelle derived by secondary endosymbiosis of an alga, and retention of the algal plastid.

show abstract

Section: Discovery Of the Apicomplexan Plastidmentioning

confidence: 99%

Mining thePlasmodiumgenome database to define organellar function: what does the apicoplast do?

Roos

Crawford

Donald

et al. 2002

Phil. Trans. R. Soc. Lond. B

View full text Add to dashboard Cite

show abstract

“…Proteins stored in these vesicles are released through the apical complex at the anterior of the cell (Sibley 2004). In addition, all apicomplexans examined to date (with the exception of the Cryptosporidium and Gregarina) possess an apicoplast organelle that is hypothesized to be an ancient secondary endosymbiosis with an algal cell (Zhu et al 2000b;Fast et al 2001;Toso and Omoto 2007). The genome of this plastid has reduced to the point where the remaining genes are predominantly involved in organelle replication (Wilson et al 1996).…”

mentioning

confidence: 99%

The origins of apicomplexan sequence innovation

Wasmuth¹,

Daub²,

Peregrín-Alvarez³

et al. 2009

Genome Res.

View full text Add to dashboard Cite

The Apicomplexa are a group of phylogenetically related parasitic protists that include Plasmodium, Cryptosporidium, and Toxoplasma. Together they are a major global burden on human health and economics. To meet this challenge, several international consortia have generated vast amounts of sequence data for many of these parasites. Here, we exploit these data to perform a systematic analysis of protein family and domain incidence across the phylum. A total of 87,736 protein sequences were collected from 15 apicomplexan species. These were compared with three protein databases, including the partial genome database, PartiGeneDB, which increases the breadth of taxonomic coverage. From these searches we constructed taxonomic profiles that reveal the extent of apicomplexan sequence diversity. Sequences without a significant match outside the phylum were denoted as apicomplexan specialized. These were collated into 9134 discrete protein families and placed in the context of the apicomplexan phylogeny, identifying the putative origin of each family. Most apicomplexan families were associated with an individual genus or species. Interestingly, many genera-specific innovations were associated with specialized host cell invasion and/or parasite survival processes. Contrastingly, those families reflecting more ancestral relationships were enriched in generalized housekeeping functions such as translation and transcription, which have diverged within the apicomplexan lineage. Protein domain searches revealed 192 domains not previously reported in apicomplexans together with a number of novel domain combinations. We highlight domains that may be important to parasite survival.

show abstract

“…Indeed, molecular and/or ultrastructural data indicates that an apicoplast is present in all major apicomplexan groups Lang-Unnasch et al, 1998). One possible exception is Cryptosporidium for which extensive searching for evidence of an apicoplast has failed to find any (Zhu et al, 2000a). This member may, therefore, have lost its apicoplast since its divergence from other apicomplexa.…”

Section: Plastid-encoded Markersmentioning

confidence: 99%

The Apicoplast: A Review of the Derived Plastid of Apicomplexan Parasites

Waller

McFadden²

2005

Current Issues in Molecular Biology

View full text Add to dashboard Cite

The apicoplast is a plastid organelle, homologous to chloroplasts of plants, that is found in apicomplexan parasites such as the causative agents of Malaria Plasmodium spp. It occurs throughout the Apicomplexa and is an ancient feature of this group acquired by the process of endosymbiosis. Like plant chloroplasts, apicoplasts are semi-autonomous with their own genome and expression machinery. In addition, apicoplasts import numerous proteins encoded by nuclear genes. These nuclear genes largely derive from the endosymbiont through a process of intracellular gene relocation. The exact role of a plastid in parasites is uncertain but early clues indicate synthesis of lipids, heme and isoprenoids as possibilities. The various metabolic processes of the apicoplast are potentially excellent targets for drug therapy.

show abstract

Cryptosporidium parvum appears to lack a plastid genome

Cited by 203 publications

References 33 publications

Mining thePlasmodiumgenome database to define organellar function: what does the apicoplast do?

Mining thePlasmodiumgenome database to define organellar function: what does the apicoplast do?

The origins of apicomplexan sequence innovation

The Apicoplast: A Review of the Derived Plastid of Apicomplexan Parasites

Contact Info

Product

Resources

About