Cryptosporiopsis radicicola sp. nov. from roots of Quercus robur

Kowalski, T.; Bartnik, Czesław

doi:10.1016/s0953-7562(09)80524-8

Cited by 20 publications

(11 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…). There were only a few species, that is Cylindrocarpon didymum , Ilyonectria radicicola, Pezicula radicicola and Sporothrix inflata , with known host preference for oak (Kowalski and Bartnik ; Halmschlager and Kowalski , ; Novotný ).…”

Section: Discussionmentioning

confidence: 99%

Fungal root endophytes ofQuercus robursubjected to flooding

2015

View full text Add to dashboard Cite

Summary In August 2011, fungal endophytes were isolated from fine (0.1–0.5 cm diameter) and structural (0.6–2.0 cm) roots from eighteen Q. robur trees at six sites in a Q. robur‐dominated forest displaying symptoms of oak decline. Five sites were subjected to periodic flooding for 3 months in 1997 and 1 month in 2010. Fungal colonization was detected in 94% of 1296 root fragments examined. Fungi representing 126 taxa from Zygomycota (four species), Ascomycota (117 species) and Basidiomycota (five species) were isolated using culture‐based methods and identified by morphotyping and sequencing of the ITS 1 and 2 rDNA. The most frequent eudominants (with colonization frequencies ≥10%) were Aspergillus spp., Cosmospora sp., Ilyonectria radicicola, Pezicula radicicola, Pyrenochaeta cava and six species of Trichoderma. Species composition did not vary greatly between sites, but only 3% of species occurred in all sites. Number of species was higher in roots subjected to floods. Eudominants and dominants present only on flooded sites included Aspergillus spp., Chaetomium globosum, Cosmospora sp., I. radicicola, Sporothrix inflata, Trichoderma harzianum, T. pubescens and T. virens. Structural roots were usually colonized by a higher number of species than fine roots. The majority of species detected belong to Class 2 NC‐endophytes. Pezicula radicicola and Phialocephala fortinii belong to Class 4 NC‐endophytes.

show abstract

Section: Discussionmentioning

confidence: 99%

Fungal root endophytes ofQuercus robursubjected to flooding

2015

View full text Add to dashboard Cite

show abstract

“…Sporendocladia bactrospora, P. fusca, and P. fluminis have conspicuous collarettes, whereas these structures are inconspicuous or absent in P. humicola and K. phycomycoides (Jong and Davis 1972;Kowalski and Bartnik 1995;Vujanovic et al 2000). Sporendocladia bactrospora deviates from the other species by its bacilliform conidia (Kendrick 1961).…”

Section: Placement Of Phialocephala and Related Species Among The Leomentioning

confidence: 99%

Genetic variability among strains of Phialocephala fortinii and phylogenetic analysis of the genus Phialocephala based on rDNA ITS sequence comparisons

Grünig¹,

Sieber²,

Rogers³

et al. 2002

Can. J. Bot.

View full text Add to dashboard Cite

Two methods to determine variability among strains of Phialocephala fortinii and sterile DSE (dark septate endophyte) Type 1 were compared: inter-simple sequence repeat polymerase chain reaction analysis (ISSR-PCR) and sequence analysis of the two internal transcribed spacers and the 5.8S ribosomal DNA (ITS1-5.8S-ITS2). Both methods showed similar results for some strains, but different results for others. Phylogenetic analysis of various strains of P. fortinii and Type 1 based on ITS1-5.8S-ITS2 sequence information showed that Type 1 strains, with the exception of one Type 1 strain and a strain with a Type-1-like culture morphology, form a well-supported clade, whereas P. fortinii strains are much more variable and the relative position of many strains on the tree are unresolved. Two sterile DSE strains with Type 1 culture morphology form a well-supported clade, separate from the P. fortinii -Type 1 clade. Phylogenetic analysis of some species of the Leotiales and of Phialocephala and related species based on ITS1-5.8S-ITS2 sequences indicate that the genera Phialocephala and Sporendocladia are polyphyletic. One major clade, supported by a high bootstrap value, is positioned as a well-separated lineage among members of the Leotiales. Phialocephala fortinii, Phialocephala dimorphospora, Phialocephala compacta, Phialocephala scopiformis, and Type 1, all known as endophytes of woody plant species, are positioned in this clade. A second well-supported clade including Phialocephala humicola, Phialocephala xalapensis, and Phialocephala fusca was positioned outside the Leotiales. Phialocephala xalapensis and P. humicola had identical sequences and were not separable based on morphology. The two taxa were, therefore, considered to represent the same species. Phialocephala virens, Phialophora finlandia, Sporendocladia foliicola, and Leptodontidium orchidicola were not included in either of the two Phialocephala clades.Résumé : Les auteurs ont comparé deux méthodes pour déterminer la variabilité entre les souches des endophytes foncés à septations (DSE) Phialocephala fortinii et des DSE stériles du Type 1 : analyse par réaction en chaîne de la polymérase avec répétition de la séquence inter-simple (ISSR-PCR) et analyse des séquences des deux espaceurs internes transcrits de l'ADN ribosomal 5.8S (ITS1-5.8S-ITS2). Les deux méthodes montrent des résultats similaires pour certaines souches, mais différent pour d'autres souches. L'analyse phylogénétique de différentes souches du P. fortinii et du Type 1, basée sur les informations venant de l'ITS1-5.8S-ITS2, montre que les souches du Type 1, à l'exception d'une souche de Type 1 et d'une souche ayant une morphologie apparentée au Type 1, forment un clade cohérent, alors que les souches du P. fortinii sont beaucoup plus variables et la position relative de plusieurs souches sur le dendrogramme demeure irrésolue. Deux souches de DSE ayant une morphologie de Type 1 forment un clade cohérent, distinct du clade P. fortinii -Type 1. L'analyse phylogénétique de certaines espèces de Lé...

show abstract

“…It is followed by a formal description of the new species from Ericaceae. Previously described Cryptosporiopsis species from roots were treated by Kowalski & Bartnik (1995), Kowalski et al (1998), andVerkley (1999).…”

Section: Key To the Species Of Cryptosporiopsis Isolated From Rootsmentioning

confidence: 99%

“…According to Kowalski (1983), the wood and bark endophyte P. cinnamomea, with its anamorph Cryptosporiopsis grisea, can also spread into the roots of dying trees. Thus far, only two Cryptosporiopsis species that were isolated exclusively from roots have been formally described based on morphological characters in vitro, viz C. radicicola from roots of Quercus robur (Kowalski & Bartnik 1995), and C. melanogena from roots of Q. robur and Q. petraea (Kowalski, Halmschlager & Schrader 1998). The teleomorphs of these species are unknown, but on the basis of partial 18S rDNA and ITS sequence analyses, Abeln et al (2000) concluded that they belong to the monophyletic genus Pezicula sensu Verkley (1999), a concept including the genus Ocellaria.…”

Section: Introductionmentioning

confidence: 99%