Cryptotanshinione Inhibits β-Amyloid Aggregation and Protects Damage from β-Amyloid in SH-SY5Y Cells

Mei, Zhengrong; Yan, Pengke; Situ, Bing; Mou, Yonggao; Liu, Peiqing

doi:10.1007/s11064-011-0652-6

Cited by 55 publications

(38 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This process is an imbalance between the systemic manifestations of RNS and ROS [12], [13]. Many neurodegenerative diseases have high levels of oxidative stress, especially those affected by ROS.…”

Section: Discussionmentioning

confidence: 99%

“…Cells (1×10 5 ) were incubated with 30 µM of Aβ peptides for 48 hours. Before conducting flow cytometry, the cells were treated with 10 µM of DCFH-DA for 30 minutes at 37°C, followed by washing with PBS [12], [13]. Data analyses were performed using program Kaluza software, version 1.2.…”

Section: Methodsmentioning

confidence: 99%

“…The neurotoxicity induced by Aβ aggregates has been associated with formation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) [12], [13]. Furthermore, neurotoxicity is highly correlated with its structural and molecular states [14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Alanine Replacement of L17 and F19 on the Aggregation and Neurotoxicity of Arctic-Type Aβ40

Chen¹,

Huang

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

Alzheimer’s disease is the most common form of neurodegenerative disease. Beta-amyloid peptides (Aβ) are responsible for neuronal death both in vitro and in vivo. Previously, L17 and F19 residues were identified as playing key roles in the stabilization of the Aβ40 conformation and in the reduction of its neurotoxicity. In this study, the effects of L17A/F19A mutations on the neurotoxicity of Aβ genetic mutant Arctic-type Aβ40(E22G) were tested. The results showed that compared to Aβ40(E22G), Aβ40(L17A/F19A/E22G) reduced the rate of conformation conversion, aggregation, and cytotoxicity, suggesting that L17 and F19 are critical residues responsible for conformational changes which may trigger the neurotoxic cascade of Aβ. Aβ40(L17A/F19A/E22G) also had decreased damage due to reactive oxygen species. The results are consistent with the discordant helix hypothesis, and confirm that residues 17–25 are in the discordant helix region. Compared to Aβ40(L17A/F19A), reduction in aggregation of Aβ40(L17A/F19A/E22G) was less significantly decreased. This observation provides an explanation based on the discordant helix hypothesis that the mutation of E22 to G22 of Aβ40(E22G) alters the propensity of the discordant helix. Arctic-type Aβ40(E22G) aggregates more severely than wild-type Aβ40, with a consequential increase in toxicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Alanine Replacement of L17 and F19 on the Aggregation and Neurotoxicity of Arctic-Type Aβ40

Chen¹,

Huang

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The proapoptotic effect of Cryptotanshinone has partially been explained by sensitisation to TRAIL [15,17]. On the other hand, Cryptotanshinone has been shown to counteract apoptosis in some cell types [18,19,20,21,22,23]. …”

Section: Introductionmentioning

confidence: 99%

Stimulation of Eryptosis by Cryptotanshinone

Bissinger

Lupescu

Zelenak

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Cryptotanshinone, a component of Salvia miltiorrhiza Bunge roots, may trigger suicidal death or apoptosis of tumor cells and has thus been recommended for the prevention and treatment of malignancy. On the other hand, Cryptotanshinone has been shown to counteract apoptosis of neurons and hepatocytes. Similar to apoptosis of nucleated cells, erythrocytes may enter eryptosis, a suicidal death characterized by cell shrinkage and phosphatidylserine translocation to the erythrocyte surface. Eryptosis may be triggered by increase of cytosolic Ca²⁺-activity ([Ca²⁺]_i). The present study explored whether Cryptotanshinone stimulates eryptosis. Methods: Forward scatter was taken as measure of cell volume, annexin V binding for identification of phosphatidylserine-exposing erythrocytes and Fluo3-fluorescence for determination of [Ca²⁺]_i. Results: A 48 h exposure of human erythrocytes to Cryptotanshinone (10 µM) was followed by significant decrease of forward scatter, significant increase of the percentage annexin-V-binding cells and significant increase of [Ca²⁺]_i. The effect of Cryptotanshinone (1 µM) on annexin-V-binding was virtually abrogated by removal of extracellular Ca²⁺. Conclusion: Cryptotanshinone is a powerful stimulator of suicidal erythrocyte death or eryptosis, which is effective mainly, if not exclusively, by stimulation of Ca²⁺ entry.

show abstract

“…16, 17 Cryptotanshinone (CTS), one of the major representative components isolated from the root of Salvia miltiorrhiza Bunge has been reported to have inhibitory effects on STAT3 activation. It was also reported to have other pharmacological effects including anticardiovascular diseases, 18 anti-inflammation, 19, 20 neuron protection 21 and antiproliferation of cancer cells. 22 We recently observed that CTS suppressed the proliferation of human MG cells T98G and U87 through inhibition of STAT3 signaling pathway.…”

mentioning

confidence: 99%

Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation

Liang

Zhang

et al. 2017

Cell Death Dis

Self Cite

View full text Add to dashboard Cite

Malignant gliomas (MGs) are one of the most common primary brain cancers in adults with a high mortality rate and relapse rate. Thus, finding better effective approaches to treat MGs has become very urgent. Here, we studied the effects of cryptotanshinone (CTS) on MGs in vitro and in vivo, and explored the underlying mechanisms. Effects of CTS in vitro on cell proliferation, cycle, migration and invasion were evaluated. The activation of JAK/STATs signaling was detected by western blot and immunofluorescenc staining. SHP-2 inhibitor or SiRNA were used to determine the involvement of SHP-2. The in vivo anti-MGs activity of CTS was studied with nude mice bearing intracerebral U87 xenografts. Our results revealed that CTS significantly inhibited the proliferation of MGs in vitro via inhibiting STAT3 signal pathway. The cell cycle was arrested at G0/G1 phase. Although CTS did not change the expression of total SHP-2 protein, the tyrosine phosphatase activity of SHP-2 protein was increased by CTS treatment in a dose-dependent manner in vivo and in vitro. SHP-2 inhibitor or SiRNA could reverse the inhibitory effect of CTS on phosphorylation of STAT3 Tyr705. In vivo study also showed that CTS inhibited the intracranial tumor growth and extended survival of nude mice bearing intracerebral U87 xenografts, confirming an inhibitory effect of CTS on MGs. Our results indicated CTS may be a potential therapeutic agent for MGs. The inhibitory action of CTS is largely attributed to the inhibition of STAT3 Tyr705 phosphorylation with a novel mechanism of upregulating the tyrosine phosphatase activity of SHP-2 protein.

show abstract

Cryptotanshinione Inhibits β-Amyloid Aggregation and Protects Damage from β-Amyloid in SH-SY5Y Cells

Cited by 55 publications

References 34 publications

Effect of Alanine Replacement of L17 and F19 on the Aggregation and Neurotoxicity of Arctic-Type Aβ40

Effect of Alanine Replacement of L17 and F19 on the Aggregation and Neurotoxicity of Arctic-Type Aβ40

Stimulation of Eryptosis by Cryptotanshinone

Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation

Contact Info

Product

Resources

About