Crystal Chemistry and Antibacterial Properties of Cupriferous Hydroxyapatite

Bhattacharjee, Arjak; Fang, Yanan; Hooper, Thomas J. N.; Kelly, Nicole L.; Gupta, Dipti; Balani, Kantesh; Manna, I.; Baikie, Tom; Bishop, P J; White, Timothy John; Hanna, John V.

doi:10.3390/ma12111814

Cited by 31 publications

(16 citation statements)

References 43 publications

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“…Radovanovic et al (2014) [ 14 ], synthetized Cu-doped BCP powders (HAp/α-TCP), and the results indicated significant antibacterial activity after 24 h of incubation for the four tested microorganisms, MSSA , E. coli , P. aeruginosa and C. albicans, compared to undoped HAp/α-TCP. Recently, Bhattacharjee et al (2019) [ 15 ]. prepared Cu-doped hydroxyapatite.…”

Section: Introductionmentioning

confidence: 99%

Copper-Doped Biphasic Calcium Phosphate Powders: Dopant Release, Cytotoxicity and Antibacterial Properties

et al. 2021

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Cytotoxicity and antibacterial properties associated with the dopant release of Cu-doped Biphasic Calcium Phosphate (BCP) powders, mainly composed of hydroxyapatite mixed with β-tricalcium phosphate powders, were investigated. Twelve BCP ceramics were synthesized at three different sintering temperatures (600 °C, 900 °C and 1200 °C) and four copper doping rates (x = 0.0, 0.05, 0.10 and 0.20, corresponding to the stoichiometric amount of copper in Ca10Cux(PO4)6(OH)2-2xO2x). Cytotoxicity assessments of Cu-doped BCP powders, using MTT assay with human-Mesenchymal Stem Cells (h-MSCs), indicated no cytotoxicity and the release of less than 12 ppm of copper into the biological medium. The antibacterial activity of the powders was determined against both Gram-positive (methicillin-sensitive (MS) and methicillin resistant (MR) Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The Cu-doped biomaterials exhibited a strong antibacterial activity against MSSA, MRSA and E. coli, releasing approximatively 2.5 ppm after 24 h, whereas 10 ppm were required to induce an antibacterial effect against P. aeruginosa. This study also demonstrated that the culture medium used during experiments can directly impact the antibacterial effect observed; only 4 ppm of Cu2+ were effective for killing all the bacteria in a 1:500 diluted TS medium, whereas 20 ppm were necessary to achieve the same result in a rich, non-diluted standard marrow cell culture medium.

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Section: Introductionmentioning

confidence: 99%

Copper-Doped Biphasic Calcium Phosphate Powders: Dopant Release, Cytotoxicity and Antibacterial Properties

et al. 2021

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“…The term “apatite” refers to a supergroup of minerals of general chemical formula [A I 4 A II 6 ][MO 4 ] 6 [X 2 ], where A 2+ is a larger cation such as Ca 2+ , within CaO 6 X and CaO 9 polyhedra, M is a smaller cation such as P (notionally P 5+ ), and X – an anion such as a halide or OH – . This supergroup includes a wide range of cation and anion substitutions, with variations in the symmetry resulting from possible cation ordering and changes to topology .…”

Section: Introductionmentioning

confidence: 99%

Apatite Thermochemistry: The Simple Salt Approximation

Glasser

2019

Inorg. Chem.

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Drouet has recently comprehensively reviewed the thermochemistry of phosphate apatites, M10(PO4)6X2. On the basis of the data assembled, he has established an optimized additive set of data values based upon the component ions, M2+, P2O5, or PO4 3–, and halide or OH– ions, which yield phosphoapatite enthalpy and Gibbs energy values generally within 1% of their experimental values. In this paper, we introduce and compare the Simple Salt Approximation (SSA) for generation of the same thermodynamic values by addition of values for the component salts of the apatite. SSA is widely applicable to ionic systems so that familiarity with this procedure is worthwhile. Drouet’s additive values focused on phosphoapatites yield better results (at 1%) than does the more general SSA (at ∼3%), but the SSA provides an alternative to data prediction when the Drouet additive factors are not available. In addition, we here use the SSA to generate approximate entropy values and estimate the Debye temperature of phosphoapatites under ambient conditions, yielding insight into the extent of activation of their vibrational modes.

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“…This study showed that the Ca/P ratio of all materials was close to human HA [ 40 ]. Furthermore, the Ca/P ratio of 1.67 is also widely used as a reference value for bone grafts, rendering it suitable for orthopedic, dental, and maxillofacial implants [ 41 , 42 ]. Thus, the Ca/Pa value of fabricated submicron BHA supported the potential of this material to be used for bone implants.…”

Section: Discussionmentioning

confidence: 99%

Fabrication and Characterization of Submicron-Scale Bovine Hydroxyapatite: A Top-Down Approach for a Natural Biomaterial

et al. 2022

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Submicron hydroxyapatite has been reported to have beneficial effects in bone tissue engineering. This study aimed to fabricate submicron-scale bovine hydroxyapatite (BHA) using the high-energy dry ball milling method. Bovine cortical bone was pretreated and calcined to produce BHA powder scaled in microns. BHA was used to fabricate submicron BHA with milling treatment for 3, 6, and 9 h and was characterized by using dynamic light scattering, scanning electron microscope connected with energy dispersive X-Ray spectroscopy, Fourier-transform infrared spectroscopy, and X-ray diffractometry to obtain its particle size, calcium-to-phosphorus (Ca/P) ratio, functional chemical group, and XRD peaks and crystallinity. Results showed that the particle size of BHA had a wide distribution range, with peaks from ~5 to ~10 µm. Milling treatment for 3, 6, and 9 h successfully gradually reduced the particle size of BHA to a submicron scale. The milled BHA’s hydrodynamic size was significantly smaller compared to unmilled BHA. Milling treatment reduced the crystallinity of BHA. However, the treatment did not affect other characteristics; unmilled and milled BHA was shaped hexagonally, had carbonate and phosphate substitution groups, and the Ca/P ratio ranged from 1.48 to 1.68. In conclusion, the fabrication of submicron-scale BHA was successfully conducted using a high-energy dry ball milling method. The milling treatment did not affect the natural characteristics of BHA. Thus, the submicron-scale BHA may be potentially useful as a biomaterial for bone grafts.

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Crystal Chemistry and Antibacterial Properties of Cupriferous Hydroxyapatite

Cited by 31 publications

References 43 publications

Copper-Doped Biphasic Calcium Phosphate Powders: Dopant Release, Cytotoxicity and Antibacterial Properties

Copper-Doped Biphasic Calcium Phosphate Powders: Dopant Release, Cytotoxicity and Antibacterial Properties

Apatite Thermochemistry: The Simple Salt Approximation

Fabrication and Characterization of Submicron-Scale Bovine Hydroxyapatite: A Top-Down Approach for a Natural Biomaterial

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