Crystal structure of juvenile hormone epoxide hydrolase from the silkworm<i>B</i><i>ombyx mori</i>

Zhou, Kang; Jia, Ning; Hu, Changwei; Jiang, Yong-Liang; Jie-pin, Yang; Chen, Yuxing; Sheng, Li; Li, Weifang; Zhou, Cong‐Zhao

doi:10.1002/prot.24676

Cited by 19 publications

(16 citation statements)

References 25 publications

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“…JHEH plays an important role in regulating insect JH titer by conversion of JH into JH diol or JHA into JHA diol ( Mackert et al 2010 ). It was first cloned from M. sexta , and belongs to the microsomal epoxide hydrolase (EC 3.3.2.9) family ( Wojtasek and Prestwich 1996 ; Zhou et al 2014 ). Here, we identified JHEH orthologs in both myriapods and chelicerates ( fig.…”

Section: Resultsmentioning

confidence: 99%

How did arthropod sesquiterpenoids and ecdysteroids arise? Comparison of hormonal pathway genes in non-insect arthropod genomes

Kenny

Lam

et al. 2015

Genome Biol Evol

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The phylum Arthropoda contains the largest number of described living animal species, with insects and crustaceans dominating the terrestrial and aquatic environments, respectively. Their successful radiations have long been linked to their rigid exoskeleton in conjunction with their specialized endocrine systems. In order to understand how hormones can contribute to the evolution of these animals, here, we have categorized the sesquiterpenoid and ecdysteroid pathway genes in the noninsect arthropod genomes, which are known to play important roles in the regulation of molting and metamorphosis in insects. In our analyses, the majority of gene homologs involved in the biosynthetic, degradative, and signaling pathways of sesquiterpenoids and ecdysteroids can be identified, implying these two hormonal systems were present in the last common ancestor of arthropods. Moreover, we found that the “Broad-Complex” was specifically gained in the Pancrustacea, and the innovation of juvenile hormone (JH) in the insect linage correlates with the gain of the JH epoxidase (CYP15A1/C1) and the key residue changes in the binding domain of JH receptor (“Methoprene-tolerant”). Furthermore, the gain of “Phantom” differentiates chelicerates from the other arthropods in using ponasterone A rather than 20-hydroxyecdysone as molting hormone. This study establishes a comprehensive framework for interpreting the evolution of these vital hormonal pathways in these most successful animals, the arthropods, for the first time.

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Section: Resultsmentioning

confidence: 99%

How did arthropod sesquiterpenoids and ecdysteroids arise? Comparison of hormonal pathway genes in non-insect arthropod genomes

Kenny

Lam

et al. 2015

Genome Biol Evol

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“…In other insect species, JHEHs are critical JH degradative enzymes (Campbell et al., ; Halarnkar et al., ; Lassiter et al., ; Khlebodarova et al., ; Debernard et al., ). On hydrolyses by JHE and JHEH, JH was degraded to form JHd and JHad (Zhou et al., ). In L. decemlineata , knockdown of Ld JHDK slowed down JH degradation.…”

Section: Discussionmentioning

confidence: 99%

CHARACTERIZATION AND FUNCTIONAL STUDY OF A PUTATIVE JUVENILE HORMONE DIOL KINASE IN THE COLORADO POTATO BEETLE Leptinotarsa decemlineata (Say)

Lü

Guo

et al. 2015

Arch Insect Biochem Physiol

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Juvenile hormone diol kinase (JHDK) is an enzyme involved in JH degradation. In the present article, a putative JHDK cDNA (LdJHDK) was cloned from the Colorado potato beetle Leptinotarsa decemlineata. The cDNA consists of 814 bp, containing a 555 bp open reading frame encoding a 184 amino acid protein. LdJHDK reveals a high degree of identity to the previously reported insect JHDKs. It possesses three conserved purine nucleotide-binding elements, and contains three EF-hand motifs (helix-loop-helix structural domains). LdJHDK mRNA was mainly detected in hindgut and Malpighian tubules. Besides, a trace amount of LdJHDK mRNA was also found in thoracic muscles, brain-corpora cardiaca-corpora allata complex, foregut, midgut, ventral ganglia, fat body, epidermis, and hemocytes. Moreover, LdJHDK was expressed throughout all developmental stages. Within the first, second, and third larval instar, the expression levels of LdJHDK were higher just before and right after the molt, and were lower in the intermediate instar. In the fourth larval instar, the highest peak of LdJHDK occurred 56 h after ecdysis. Ingestion of double-stranded RNA (dsRNA) against LdJHDK successfully knocked down the target gene, increased JH titer, and significantly upregulated LdKr-h1 mRNA level. Knockdown of LdJHDK significantly impaired adult emergence. Thus, we provide a line of experimental evidence in L. decemlineata to support that LdJHDK encodes function protein involved in JH degradation.

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“…The N-terminal membrane anchor motif "XWG" is labeled as triangles. The structure, α-helices and β-sheets, are indicated above the alignment according to the result from B. mori (Zhou et al, 2014). The catalytic triad (Asp225, His429, and Asp404), HGWP motif and two tyrosine residues (Tyr297 and Tyr374) are labeled as stars (the amino acid position is in LdJHEH1).…”

Section: Effect Of Dsjheh1 Dsjheh2 and Their Mixture On Jh Signalingmentioning

confidence: 99%

Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle

Lü

Guo

et al. 2015

Gene

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Crystal structure of juvenile hormone epoxide hydrolase from the silkwormBombyx mori

Cited by 19 publications

References 25 publications

How did arthropod sesquiterpenoids and ecdysteroids arise? Comparison of hormonal pathway genes in non-insect arthropod genomes

How did arthropod sesquiterpenoids and ecdysteroids arise? Comparison of hormonal pathway genes in non-insect arthropod genomes

CHARACTERIZATION AND FUNCTIONAL STUDY OF A PUTATIVE JUVENILE HORMONE DIOL KINASE IN THE COLORADO POTATO BEETLE Leptinotarsa decemlineata (Say)

Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle

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