Crystal structure of mouse CD11b I-domain (CD11b-I) in complex with Staphylococcus aureus octameric bi-component leukocidin LukGH (LukH K319A mutant)

Trstenjak, Nikolina; Milić, Dalibor; Djinović-Carugo, Kristina; Badarau, Adriana

doi:10.2210/pdb6rhv/pdb

2019

DOI: 10.2210/pdb6rhv/pdb

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Crystal structure of mouse CD11b I-domain (CD11b-I) in complex with Staphylococcus aureus octameric bi-component leukocidin LukGH (LukH K319A mutant)

Nikolina Trstenjak¹,

Dalibor Milić²,

Kristina Djinović-Carugo³

et al.

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Molecular mechanism of leukocidin GH–integrin CD11b/CD18 recognition and species specificity

Trstenjak

Milić

Graewert

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Host–pathogen interactions are central to understanding microbial pathogenesis. The staphylococcal pore-forming cytotoxins hijack important immune molecules but little is known about the underlying molecular mechanisms of cytotoxin–receptor interaction and host specificity. Here we report the structures of a staphylococcal pore-forming cytotoxin, leukocidin GH (LukGH), in complex with its receptor (the α-I domain of complement receptor 3, CD11b-I), both for the human and murine homologs. We observe 2 binding interfaces, on the LukG and the LukH protomers, and show that human CD11b-I induces LukGH oligomerization in solution. LukGH binds murine CD11b-I weakly and is inactive toward murine neutrophils. Using a LukGH variant engineered to bind mouse CD11b-I, we demonstrate that cytolytic activity does not only require binding but also receptor-dependent oligomerization. Our studies provide an unprecedented insight into bicomponent leukocidin–host receptor interaction, enabling the development of antitoxin approaches and improved animal models to explore these approaches.

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Molecular mechanism of leukocidin GH–integrin CD11b/CD18 recognition and species specificity

Trstenjak

Milić

Graewert

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

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Crystal structure of mouse CD11b I-domain (CD11b-I) in complex with Staphylococcus aureus octameric bi-component leukocidin LukGH (LukH K319A mutant)

Cited by 1 publication

References 0 publications

Molecular mechanism of leukocidin GH–integrin CD11b/CD18 recognition and species specificity

Molecular mechanism of leukocidin GH–integrin CD11b/CD18 recognition and species specificity

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