Crystal structure of QscR, a<i>Pseudomonas aeruginosa</i>quorum sensing signal receptor

Lintz, Mario J.; Oinuma, Ken Ichi; Wysoczynski, Christina L.; Greenberg, E. Peter; Churchill, Mair E. A.

doi:10.1073/pnas.1112398108

Cited by 113 publications

(152 citation statements)

References 47 publications

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“…The potential role of each of these receptors in competition has not been determined. AHL receptor specificity can be easily altered by single amino-acid changes (Collins et al, 2005;Hawkins et al, 2007;Chen et al, 2011;Lintz et al, 2011), suggesting that AHL recognition may be very adaptable in nature. In contrast to these broad-specificity AHL receptors, the receptor of the squid symbiont Vibrio fischeri is quite specific for its cognate AHL (Visick and Ruby, 1999).…”

Section: Discussionmentioning

confidence: 99%

Acyl-homoserine lactone-dependent eavesdropping promotes competition in a laboratory co-culture model

et al. 2012

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Many Proteobacteria use acyl-homoserine lactone (AHL)-mediated quorum sensing to activate the production of antibiotics at high cell density. Extracellular factors like antibiotics can be considered public goods shared by individuals within a group. Quorum-sensing control of antibiotic production may be important for protecting a niche or competing for limited resources in mixed bacterial communities. To begin to investigate the role of quorum sensing in interspecies competition, we developed a dual-species co-culture model using the soil saprophytes Burkholderia thailandensis (Bt) and Chromobacterium violaceum (Cv). These bacteria require quorum sensing to activate the production of antimicrobial factors that inhibit growth of the other species. We demonstrate that quorum-sensing-dependent antimicrobials can provide a competitive advantage to either Bt or Cv by inhibiting growth of the other species in co-culture. Although the quorum-sensing signals differ for each species, we show that the promiscuous signal receptor encoded by Cv can sense signals produced by Bt, and that this ability to eavesdrop on Bt can provide Cv an advantage in certain situations. We use an in silico approach to investigate the effect of eavesdropping in competition, and show conditions where early activation of antibiotic production resulting from eavesdropping can promote competitiveness. Our work supports the idea that quorum sensing is important for interspecies competition and that promiscuous signal receptors allow eavesdropping on competitors in mixed microbial habitats.

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Section: Discussionmentioning

confidence: 99%

Acyl-homoserine lactone-dependent eavesdropping promotes competition in a laboratory co-culture model

et al. 2012

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“…This is in agreement with studies demonstrating that even minor changes in the length or substituents of the acyl tail, which contributes to the binding affinity between the receptor and the ligand (11,166,(194)(195)(196), can have large effects on its activity (11,170,197). Alteration of the chirality of the lactone ring or substitutions of part or all of the lactone head group can also alter the activity of a given molecule for a given AHL receptor (11, 118, 165-167, 196, 197), likely because of important conserved intermolecular hydrogen bonds between the AHL lactone head group and the ligand-binding pockets of AHL receptors (175)(176)(177)(178)(179)(180). In addition to promoting ligand-receptor interaction, hydrogen bonding may also help to determine the type of activity exhibited by a given compound.…”

Section: Inhibition Of Signal Detection Synthetic Signal Analogues Anmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

690

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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“…5). Current structures of LuxR-type regulators show that their dimerization is mainly mediated by their LBDs, whereas their DBDs have a minor role for their dimerization (15,(26)(27)(28). The LBD dimerization of LuxR-type regulators may help to position the DBD dimer in a correct conformation to recognize the target DNA sequence.…”

Section: Resultsmentioning

confidence: 99%

QsIA disrupts LasR dimerization in antiactivation of bacterial quorum sensing

Fan

Dong

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Significance Quorum sensing is a bacterial cell–cell communication system that is activated when the concentration of quorum sensing signal (autoinducer) reaches a threshold. In Pseudomonas aeruginosa , an opportunistic human pathogen, the quorum sensing threshold and response are defined by a novel antiactivator, QslA, which binds to the transcription factor LasR and prevents it from binding to its target DNA. However, how QslA binds to LasR and negatively regulates quorum sensing is poorly understood. Here we show that QsIA binds LasR to disrupt its dimerization, thereby inhibiting the DNA binding of LasR and shutting down transcription. Our findings reveal the molecular basis of a unique QsIA-mediated LasR inactivation and add an example to understand the antiactivation mechanism in bacterial quorum sensing.

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Crystal structure of QscR, aPseudomonas aeruginosaquorum sensing signal receptor

Cited by 113 publications

References 47 publications

Acyl-homoserine lactone-dependent eavesdropping promotes competition in a laboratory co-culture model

Acyl-homoserine lactone-dependent eavesdropping promotes competition in a laboratory co-culture model

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

QsIA disrupts LasR dimerization in antiactivation of bacterial quorum sensing

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